The objective of this study was to characterize the variability of spectral reflectance and temporal profiles of vegetation indices associated with nitrogen fertilization, crop cycle periods, and weather conditions of the growing season in canola canopies in southern Brazil. An experiment was carried out during the 2013 and 2014 canola growing seasons at EMBRAPA Trigo, Passo Fundo, state of Rio Grande do Sul, Brazil. The experiment was conducted in a randomized block design with four replications. Five doses of nitrogen top dressing were used as treatments: 10, 20, 40, 80, and 160kg ha-1. Measurements were obtained with the spectroradiometer positioned above the canopy, to construct spectral reflectance curves for canola and establish temporal profiles for several vegetation indices (SR, NDVI, EVI, SAVI, and GNDVI). In addition, data on shoot dry matter were obtained and phenological stages were determined. The spectral reflectance curves of canola were reported to change with canopy growth and development. Temporal profiles of vegetation indices showed two maximum peaks, one before flowering and other after flowering. The indices SR, NDVI, EVI, SAVI, and GNDVI were able to characterize changes in the canola canopy over time, as a function of phenological phases, weather conditions, and nitrogen fertilization, throughout the development cycle. Plant growth and development, variations in crop management, and environmental conditions affect the spectral response of canola.