Identification of cross-reactive antigens between Mycoplasma pulmonis and Mycoplasma arthritidis

Minion, F. Chris; Brown, Mary Bomberger; Cassell, Gail H.

doi:10.1128/iai.43.1.115-121.1984

Cited by 41 publications

(16 citation statements)

References 17 publications

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“…In fact, it is well known that M. pneumoniae has antigenic components similar to those of certain bacteria, to some animal and human tissues (1,11), and even to glycolipids from parsnips, carrots and spinach (11). Furthermore, it has recently been demonstrated by ELISA and other methods that mycoplasmas have cross-reactive antigens among some species of mycoplasmas (5,8,10). Using ELISA and immunoblotting, we also confirmed the existence of cross-reactive antibodies to mycoplasmas in the sera of patients infected with M. pneumoniae (manuscript in preparation).…”

Section: Elisa Activity Of Human Sera For Some Mycoplasmassupporting

confidence: 73%

Cross‐Reactive Antibodies to Mycoplasmas Found in Human Sera by the Enzyme‐Linked Immunosorbent Assay (ELISA)

Sasaki

Bonissol

Stoiljkovic

1987

Microbiology and Immunology

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Antibodies against Mycoplasma pneumoniae in patients' sera with M. pneumoniae infection were measured by the complement fixation (CF) test and enzyme‐linked immunosorbent assay (ELISA). Many patients' sera cross‐reacted with heterologous mycoplasmal ELISA antigens such as M. hominis, M. hyorhinis, M. orale, M. pulmonis and M. salivarium. The sera with high CF (CF≥40) titers gave significantly higher ELISA values to M. hyorhinis (P<0.001) and M. pulmonis (P<0.001), which are not parasitic for humans, than those with low CF (CF<20) titer. Human normal immunoglobulin G (human normal IgG) containing 98% or more IgG, prepared from pooled plasma of at least 500 normal human donors, showed ELISA reactions with all mycoplasmal strains used. The nonspecific adsorption of human normal IgG on the surface of plate wells and on medium components which might contaminate mycoplasmal ELISA antigens could be disregarded. These results suggest that cross‐reactive antibodies to mycoplasmas exist in human sera, and they affect the results of ELISA for serodiagnosis of M. pneumoniae infection.

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Section: Elisa Activity Of Human Sera For Some Mycoplasmassupporting

confidence: 73%

Cross‐Reactive Antibodies to Mycoplasmas Found in Human Sera by the Enzyme‐Linked Immunosorbent Assay (ELISA)

Sasaki

Bonissol

Stoiljkovic

1987

Microbiology and Immunology

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“…arthritidis ) that cross-react with M . pulmonis antigenically 15 . Natural infections with other Mycoplasma species in mice and rats are rarely reported, however, and most samples that tested positive by IFA using M .…”

Section: Discussionmentioning

confidence: 99%

A serological survey to evaluate contemporary prevalence of viral agents and Mycoplasma pulmonis in laboratory mice and rats in western Europe

Mähler

Köhl

2009

Lab Anim

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To evaluate current prevalence rates of 24 viruses and of the bacterium Mycoplasma pulmonis, the authors retrospectively surveyed serological data obtained from laboratory mice and rats housed in more than 100 western European institutions. Serum samples were submitted to the authors' institution for testing between January 2007 and June 2008. The prevalence of an infection was defined as the percentage of tested samples that yielded positive results for a specific agent. In mice, the most commonly detected infectious agents were murine norovirus (prevalence of 31.8%), mouse hepatitis virus (5.5%), mouse rotavirus (1.7%) and parvoviruses (1.0%). In rats, parvoviruses (12.1%) and M. pulmonis (3.6%) were the most prevalent infectious agents. Most rodent parvovirus infections could be attributed to mouse parvovirus in mice and to rat minute virus or to Kilham rat virus in rats. These data suggest the importance of up-to-date animal health monitoring programs and should stimulate the scientific community to further improve the microbiological quality of laboratory rodents.

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“…Additional dilutions were not possible because of volume limitations. Samples were tested for the presence of specific antibody by a microtiter ELISA as described previously (14). Flatbottomed microtiter wells were coated with 0.2 ml of M. pulmonis antigen (10 p,g of protein per ml).…”

Section: Brown and Reyesmentioning

confidence: 99%

Immunoglobulin class- and subclass-specific responses to Mycoplasma pulmonis in sera and secretions of naturally infected Sprague-Dawley female rats

Brown

Reyes

1991

Infect Immun

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Mycoplasma pulmonis causes chronic murine respiratory mycoplasmosis and genital disease in rats. Specffic immunoglobulin M (IgM), IgA, and IgG and its subclasses present in sera and tracheal and uterine lavage samples from 36 naturally infected Sprague-Dawley female rats were tested for reactivity with M. pulmonis in an enzyme-linked immunosorbent assay. Ten specific-pathogen-free Sprague-Dawley female rats served as the negative controls. Tracheal and uterine lavage samples were cultured quantitatively for M. pulmonis. M. pulmonis was isolated from the trachea (35 of 36) and uterus (17 of 36) of naturally infected rats; all rats were infected in at least one of the two sites cultured. M. pulmonis was not isolated from any control rat. There was a significant difference in levels of specific antibody of all classes except IgG2c between control and naturally infected animals (P < 0.001 for IgM, IgG, IgGl, and IgG2a; P < 0.002 for IgG2b; and P < 0.02 for IgA). There was no correlation between numbers of M. pulmonis cells isolated and the amount or class of antibody measured in serum or tracheal lavage specimens. The predominant antibodies to M. pulmonis found in the sera of naturally infected rats were IgG and IgM. The IgG2a subclass was responsible for the majority of IgGpositive animals. There were no differences between rats which were positive by culture for M. pulmonis in the uterus (U+) and rats which were negative by culture for M. pulmonis in the uterus (U-) with respect to distribution or amount of antibody classes and subclasses in the serum. However, tracheal wash samples from U+ rats had significantly higher (P < 0.03) levels of specific IgGl and IgG2a than those from U-rats.Conversely, IgG2a was present in higher levels in pooled uterine lavage specimens from U-rats than in those from U+ rats.

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Identification of cross-reactive antigens between Mycoplasma pulmonis and Mycoplasma arthritidis

Cited by 41 publications

References 17 publications

Cross‐Reactive Antibodies to Mycoplasmas Found in Human Sera by the Enzyme‐Linked Immunosorbent Assay (ELISA)

Cross‐Reactive Antibodies to Mycoplasmas Found in Human Sera by the Enzyme‐Linked Immunosorbent Assay (ELISA)

A serological survey to evaluate contemporary prevalence of viral agents and Mycoplasma pulmonis in laboratory mice and rats in western Europe

Immunoglobulin class- and subclass-specific responses to Mycoplasma pulmonis in sera and secretions of naturally infected Sprague-Dawley female rats

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