Identification of crucial noncoding RNAs and mRNAs in hypertrophic scars via RNA sequencing

Abstract

Hypertrophic scaring (HS) is a dermal fibroproliferative disorder characterized by excessive deposition of collagen and other extracellular matrix (ECM) components. The aim of this study is to explore crucial lncRNAs and circRNAs associated with HS and provide a better understanding of the molecular mechanism of HS. To investigate the lncRNA, circRNA and mRNA expression profiles we performed RNA-sequencing of human HS and normal skin tissues. Following the identification of differentially expressed mRNAs (DEmRNAs), lncRNAs (DElncRNAs) and circRNAs (DEcircRNAs), we performed functional enrichment of DEmRNAs. Further on, we constructed DElncRNA/DEcircRNA-DEmRNA co-expression networks and ceRNA regulatory networks and performed functional analyses of the DEmRNAs in the constructed networks. In total, 487 DEmRNAs, 92 DElncRNAs and 17 DEcircRNAs were identified. DEmRNAs were significantly enriched in processes like collagen fibril organization, ECM-receptor interaction and PI3K-Akt signaling pathway. Additionally, we detected 580 DElncRNA-DEmRNA and 505 DEcircRNA-DEmRNA co-expression pairs The ceRNA network contained 18 circRNA-miRNA pairs, 18 lncRNA-miRNA pairs and 409 miRNA-mRNA pairs, including 10 circRNAs, 5 lncRNAs, 15 miRNAs, and 160 mRNAs. We concluded that MIR503HG/hsa-miR-204-3p/ACAN, MIR503HG/hsa-miR-431-5p/TNFRSF9, MEG3/hsa-miR-6884-5p/ADAMTS14, AC000035.1-ADAMTS14 and hsa_circ_0069865-COMP/ADAM12 interaction pairs may play a central role in HS.

Upregulation of long noncoding RNA linc02544 and its association with overall survival rate and the influence on cell proliferation and migration in lung squamous cell carcinoma

“…The abnormal expression of linc02544 was reported in several diseases [ 12 , 13 ]. For instance, a study performed RNA sequencing and acquired many differently expressed lncRNAs, mRNAs, and circRNAs in the human hypertrophic scar, in which linc02544 was one of the top three deregulated expression lncRNAs, which may play a central role in hypertrophic scar [ 12 ].…”

“…The abnormal expression of linc02544 was reported in several diseases [ 12 , 13 ]. For instance, a study performed RNA sequencing and acquired many differently expressed lncRNAs, mRNAs, and circRNAs in the human hypertrophic scar, in which linc02544 was one of the top three deregulated expression lncRNAs, which may play a central role in hypertrophic scar [ 12 ]. Linc02544, a full-length 544 nt non-coding RNA, is highly expressed in breast cancer and may be an application prognostic marker for neoadjuvant therapy patients with breast cancer [ 21 ].…”

“…The aberrant expression of linc02544 was observed in several diseases, such as hypertrophic scars [ 12 ] and breast cancer [ 13 ]. A recent study identified numerous lncRNAs and mRNAs in lung adenocarcinoma, among which include linc02544 [ 14 ].…”

The injury-induced circular RNA circGLIS3 activates dermal fibroblasts to promote wound healing

“…Interestingly, here we identified circGLIS3 to be highly upregulated in keloid lesions compared to the healthy skin found in proximity. Similarly, HTS tissues were shown to contain higher circGLIS3 levels compared to healthy skin tissue ( 35 ). These lines of evidence highlight that, besides its beneficial role for wound closure, circGLIS3 may also regulate dermal fibrosis and represent a therapeutic target in keloids and HTS.…”

Beyond the Code: Noncoding RNAs in Skin Wound Healing

“…Additionally, a changed circRNA expression profile was observed in a diabetic wound mouse model treated with Sheng-ji Hua-yu, a traditional Chinese herbal promoting wound healing (Xiang et al 2021). Abnormal circRNA expression pro- files have also been found in human HSs and keloids (Li et al 2018c(Li et al , 2021cShi et al 2020a;Zhang et al 2020bZhang et al , 2021a. In particular, lower hsa_circRNA_0008259 expression is detected in keloid fibroblasts, and this circRNA can suppress type I and III collagen production (Zhang et al 2020b).…”