Identification of CTX-M15-, SHV-28-producing Klebsiella pneumoniae ST15 as an epidemic clone in the Copenhagen area using a semi-automated Rep-PCR typing assay

Nielsen, Julius; Skov, Marianne Nielsine; Jørgensen, Rikke Lind; Heltberg, Ole; Hansen, Dennis Schrøder; Schønning, Kristian

doi:10.1007/s10096-011-1153-x

Cited by 59 publications

(38 citation statements)

References 18 publications

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“…ST15 together with ST326 caused the CG15 predominance among the rectal colonizers in GI in Barcelona (48.0%), in contrast to ESBL-or AmpC-producing K. pneumoniae clinical and carrier isolates from other Spanish centers (13,31). CG15, usually ST15 and/or ST14 (not found here), has been commonly observed with ESBLs or other ␤-lactamases worldwide (12,16,18,(32)(33)(34). CC147 was identified in four hospitals and was the largest CG/CC overall (n ϭ 42; 18.3%).…”

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confidence: 55%

Comparative Population Analysis of Klebsiella pneumoniae Strains with Extended-Spectrum β-Lactamases Colonizing Patients in Rehabilitation Centers in Four Countries

Baraniak

Izdebski

Fiett

et al. 2013

Antimicrob Agents Chemother

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The international project MOSAR was conducted in five rehabilitation centers; patients were screened for rectal carriage of extended-spectrum ␤-lactamase (ESBL)-producing members of the Enterobacteriaceae. Among 229 Klebsiella pneumoniae isolates, four clonal groups (CG) or complexes (CC) prevailed: CG17 in France, CG101 in Italy, CG15 in Spain, and CC147 in Israel. ESBLs, mainly CTX-Ms, were produced by 226 isolates; three isolates expressed AmpC-like cephalosporinases. High genetic diversity of K. pneumoniae populations was observed, with specific characteristics at each center.

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confidence: 55%

Comparative Population Analysis of Klebsiella pneumoniae Strains with Extended-Spectrum β-Lactamases Colonizing Patients in Rehabilitation Centers in Four Countries

Baraniak

Izdebski

Fiett

et al. 2013

Antimicrob Agents Chemother

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“…Beside several minor clones, three internationally disseminated CTX-M-15 producing K. pneumoniae clones, namely ST15, ST101 and ST147 were present in the Clinical Centre University of Pécs, of which ST15 and ST147 are considered to be epidemic clones in Hungary [6,[32][33][34][35][36].…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of CTX-M-15 producing Klebsiella pneumoniae clone ST101 in a Hungarian university teaching hospital

Melegh

Schneider

Horváth

et al. 2015

Acta Microbiologica et Immunologica Hungarica

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We investigated the molecular epidemiology of extended spectrum β-lactamase (ESBL) producing Klebsiella pneumoniae isolates derived from the teaching hospitals of University of Pécs, Pécs, Hungary in the time period [2004][2005][2006][2007][2008]. Molecular typing, antimicrobial susceptibility testing, detection of common β-lactamase genes (bla CTX-M , bla TEM and bla SHV ) and virulence associated traits (hypermucoviscosity, magA, k2a, rmpA, siderophores, type 1 and 3 fi mbria, biofi lm formation, serum resistance) were performed for 102 isolates. The results showed the presence of three major ciprofl oxacin resistant CTX-M-15 producing clones (ST15 n = 69, ST101 n = 10, and ST147 n = 9), of which ST15 was predominant and universally widespread. Considering distribution in time and place, ST101 and ST147 were detected at fewer inpatient units and within a narrower time frame, as compared to ST15. Beside major clones, eleven minor clones were identifi ed, and were shown to harbour the following β-lactamase genes: six clones carried bla CTX-M , four clones harboured bla SHV-5 and one clone possessed both bla CTX-M and ESBL type bla SHV . Among the SHV-5 producing K. pneumoniae clones a novel sequence type was found, namely ST1193, which harboured a unique infB allele. Different virulence factor content and peculiar antimicrobial susceptibility profi le were characteristic for each clone. In contrast to major clone isolates, which showed high level resistance to ciprofl oxacin, minor clone *Corresponding author; E-mail: melegh.szilvia@pte.hu 234 MELEGH et al.Acta Microbiologica et Immunologica Hungarica 62, 2015 isolates displayed signifi cantly lower MIC values for ciprofl oxacin suggesting a role for fl uoroquinolones in the dissemination of the major K. pneumoniae clones. This is the fi rst description of the CTX-M-15 producing K. pneumoniae clone ST101 in Hungary.

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“….the key challenge will not be to produce the sequence data, but to rapidly compute and interpret the relevant information from large data sets" (94). Easy-to-use criteria to define which isolates are within the same clonal lineage or within the same hospital outbreak are currently available for band-based techniques (23,40,48) but not for WGS (104). For this reason, we identified a threshold of 2,500 core SNPs that distinguished isolates of the same clonal lineage from those of different clonal lineages.…”

Section: Figmentioning

confidence: 99%

“…The Dice statistic was used, and band tolerance was set to 1%. Isolates clustering together at Ͼ90% similarity based on the UPGMA dendrogram were considered to be the same type (23,48,49). Whole-genome sequencing and assembly.…”

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Utility of Whole-Genome Sequencing in Characterizing Acinetobacter Epidemiology and Analyzing Hospital Outbreaks

2016

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bAcinetobacter baumannii frequently causes nosocomial infections and outbreaks. Whole-genome sequencing (WGS) is a promising technique for strain typing and outbreak investigations. We compared the performance of conventional methods with WGS for strain typing clinical Acinetobacter isolates and analyzing a carbapenem-resistant A. baumannii (CRAB) outbreak. We performed two band-based typing techniques (pulsed-field gel electrophoresis and repetitive extragenic palindromic-PCR), multilocus sequence type (MLST) analysis, and WGS on 148 Acinetobacter calcoaceticus-A. baumannii complex bloodstream isolates collected from a single hospital from 2005 to 2012. Phylogenetic trees inferred from core-genome single nucleotide polymorphisms (SNPs) confirmed three Acinetobacter species within this collection. Four major A. baumannii clonal lineages (as defined by MLST) circulated during the study, three of which are globally distributed and one of which is novel. WGS indicated that a threshold of 2,500 core SNPs accurately distinguished A. baumannii isolates from different clonal lineages. The bandbased techniques performed poorly in assigning isolates to clonal lineages and exhibited little agreement with sequence-based techniques. After applying WGS to a CRAB outbreak that occurred during the study, we identified a threshold of 2.5 core SNPs that distinguished nonoutbreak from outbreak strains. WGS was more discriminatory than the band-based techniques and was used to construct a more accurate transmission map that resolved many of the plausible transmission routes suggested by epidemiologic links. Our study demonstrates that WGS is superior to conventional techniques for A. baumannii strain typing and outbreak analysis. These findings support the incorporation of WGS into health care infection prevention efforts.A n important role of clinical microbiology is to identify relationships between bacterial isolates. At a broad level, phenotypic and genotypic tests are used to categorize bacterial isolates into the same or different species. Within a bacterial species, techniques are used to group isolates into clonal lineages, which are groups of closely related bacteria that share a recent common ancestor but have spread regionally or globally. At a more local level, infection control practitioners must determine whether a group of isolates constitutes a hospital outbreak by ascertaining whether the isolates have a degree of similarity consistent with a common source within the hospital. Once isolates belonging to a hospital outbreak have been identified, similarities and differences between these isolates can be exploited to generate a transmission map to aid in finding the source of the outbreak and in disrupting ongoing pathways of transmission. For some groups of bacteria, such as Acinetobacter, discernment at each level has medically important consequences and therefore must be accomplished by hospital-associated clinical microbiology laboratories.Within the Acinetobacter genus, the Acinetobacter calcoaceticusAcinetobac...

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Identification of CTX-M15-, SHV-28-producing Klebsiella pneumoniae ST15 as an epidemic clone in the Copenhagen area using a semi-automated Rep-PCR typing assay

Cited by 59 publications

References 18 publications

Comparative Population Analysis of Klebsiella pneumoniae Strains with Extended-Spectrum β-Lactamases Colonizing Patients in Rehabilitation Centers in Four Countries

Comparative Population Analysis of Klebsiella pneumoniae Strains with Extended-Spectrum β-Lactamases Colonizing Patients in Rehabilitation Centers in Four Countries

Identification and characterization of CTX-M-15 producing Klebsiella pneumoniae clone ST101 in a Hungarian university teaching hospital

Utility of Whole-Genome Sequencing in Characterizing Acinetobacter Epidemiology and Analyzing Hospital Outbreaks

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