Identification of D group chromosomes by autoradiography and fluorescence microscopy

Abstract

Identification of D group chromosomes was done by quinacrine mustard staining and fluorescence microscopy, as well as by 3H–thymidine labeling and autoradiography. No special instruments were used for the fluorescence microscopy studies. Chromosome 13 was recognized easily by fluorescence microscopy by its typical fluorescence pattern in the lower two–thirds of the long arm, the region of the late labeling in autoradiography. Chromosomes 14 and 15 could be recognized by fluorescence microscopy in most cases. If differentiation is not quite definite, 3H–thymidine labeling and autoradiography should be used to support the findings.

Localization of male determining factor on short arm of Y chromosome Case report of a baby with 46, x, t (Yp+;14q‐)

“…Skin A 1-3 cullures of the propositus were done from autopsy material; the chromosomes were obtained by conventional methods. The methods used for fluorescence microscopy were described previously (Krmpotic et al 1971).…”