Identification of DAF1/CD55, a Novel Definitive Endoderm Marker

Shiraki, Nobuaki; Harada, Seiko; Ogaki, Soichiro; Kume, Kazuhiko; Kume, Shoen

doi:10.1247/csf.10004

Cited by 14 publications

(20 citation statements)

References 17 publications

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“…Decay accelerating factor (DAF1/CD55), a gene found to be highly expressed in ES cell-derived DE, was detected in the DE and mesoderm in early embryos at E8.5 [49]. Flow cytometry analysis of ES cell-derived differentiated cells revealed that DAF1+ cells also expressed CXCR4 on the cell surface.…”

Section: Identification Of De-specific Genes In Es Cell-differentiationmentioning

confidence: 99%

Profiling of Embryonic Stem Cell Differentiation

2014

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■ AbstractEmbryonic stem (ES) cells have been shown to recapitulate normal developmental stages. They are therefore a highly useful tool in the study of developmental biology. Profiling of ES cell-derived cells has yielded important information about the characteristics of differentiated cells, and allowed the identification of novel marker genes and pathways of differentiation. In this review, we focus on recent results from profiling studies of mouse embryos, human islets, and human ES cell-derived differentiated cells from several research groups. Global gene expression data from mouse embryos have been used to identify novel genes or pathways involved in the developmental process, and to search for transcription factors that regulate direct reprogramming. We introduce gene expression databases of human pancreas cells (Beta Cell Gene Atlas, EuroDia database), and summarize profiling studies of islet-or human ES cell-derived pancreatic cells, with a focus on gene expression, microRNAs, epigenetics, and protein expression. Then, we describe our gene expression profile analyses and our search for novel endoderm, or pancreatic, progenitor marker genes. We differentiated mouse ES cells into mesendoderm, definitive endoderm (DE), mesoderm, ectoderm, and Pdx1-expressing pancreatic lineages, and performed DNA microarray analyses. Genes specifically expressed in DE, and/or in Pdx1-expressing cells, were extracted and their expression patterns in normal embryonic development were studied by in situ hybridization. Out of 54 genes examined, 27 were expressed in the DE of E8.5 mouse embryos, and 15 genes were expressed in distinct domains in the pancreatic buds of E14.5 mouse embryos. Akr1c19, Aebp2, Pbxip1, and Creb3l1 were all novel, and none has been described as being expressed, either in the DE, or in the pancreas. By introducing the profiling results of ES cell-derived cells, the benefits of using ES cells to study early embryonic development will be discussed.

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Section: Identification Of De-specific Genes In Es Cell-differentiationmentioning

confidence: 99%

Profiling of Embryonic Stem Cell Differentiation

2014

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“…Moreover, we found that Decay Accelerating Factor (DAF1/CD55) as a candidate surface marker for the identification of the early and late definitive endoderm [34]. These results strongly suggest that M15 differentiation system is useful to analyze early developmental steps in vitro.…”

Section: The Mesonephric Cell Line M15 Can Induce the Mouse Es Or Imentioning

confidence: 64%

“…cells are shown to mark the definitive population [32,33]. We recently identified a novel definitive endoderm marker CD55/DAF1 [34], which expression was maintained in more matured endoderm cells.…”

Section: Lineage Specific Generation Of the Definitive Endoderm Cellsmentioning

confidence: 99%

Xenopus Embryos and ES Cells as Tools for Studies of Developmental Biology

Kume

2010

Neurochem Res

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Nearly 20 years ago Professor Katsuhiko Mikoshiba led me to an exciting world of IP(3)-Ca(2+) signaling, we embarked on the role of IP(3)-Ca(2+) signaling on fertilization, early cell cycle progression, and body axis formation. I was fully enchanted by the world of basic science, particularly developmental biology. It is a great pleasure to contribute a paper to this special issue of Neurochemical Research honoring Professor Katsuhiko Mikoshiba. Many of the former lab members are now working in a wide range of fields, both inside or outside the fields of Neurochemical research. I am one of those who are working in a different field. Therefore, it seems fitting here to first write about our former work with IP3 receptor, and then introduce our recent works.

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“…However, Cxcr4 expression decreases during development, making the isolation of late definitive endoderm cells difficult. The combination of E-cadherin with CD55 has circumvented this problem by identifying early and late definitive endoderm cells in the embryo, and in ESC-derived cell cultures where GFP is expressed under the promoter of Pdx1 (Shiraki, Harada et al 2010). Our group has also observed the presence of E-cadherin + CD55 + cells in our ESC-IPC cultures ( Figure 4B).…”

Section: Definitive Endoderm Progenitorsmentioning

confidence: 79%

“…In mice, putative definitive endodermal cells that co-express E-cadherin and Decay Accelerating Factor (DAF1/CD55) cell surface markers also express Pdx1 (Shiraki, Harada et al 2010). Discoidin domain receptor tyrosine kinase 1 (DDR1) and delta/notch-like EGF related receptor (DNER) have been also indicated as possible murine pancreatic progenitor surface markers with bioinformatics studies confirmed by immunohistochemistry (Hald, Galbo et al 2011).…”

Section: Definitive Endoderm Progenitorsmentioning

confidence: 98%