2012
DOI: 10.1016/j.mimet.2012.10.003
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Identification of dairy lactic acid bacteria by tRNAAla–23S rDNA-RFLP

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Cited by 13 publications
(10 citation statements)
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“…A specific ARDRA [tRNAAla-23S rDNA-restriction fragment length polymorphism (RFLP)] was recently applied by Mancini et al (2012), to identify isolates collected during the production and ripening of GP cheese. This method, based on a restriction of a specific fragment of 16S–23S rDNA, represents a valid option for dairy LAB screening and identification.…”
Section: Molecular Approaches For Analyzing Raw-milk Long-ripened Chmentioning
confidence: 99%
See 1 more Smart Citation
“…A specific ARDRA [tRNAAla-23S rDNA-restriction fragment length polymorphism (RFLP)] was recently applied by Mancini et al (2012), to identify isolates collected during the production and ripening of GP cheese. This method, based on a restriction of a specific fragment of 16S–23S rDNA, represents a valid option for dairy LAB screening and identification.…”
Section: Molecular Approaches For Analyzing Raw-milk Long-ripened Chmentioning
confidence: 99%
“…The most powerful of these are DNA fingerprinting techniques: RFLP of protein-coding genes involved in primary metabolism (Giraffa et al, 2003) and 16S rRNA (Gala et al, 2008), restriction enzyme analysis pulsed-field gel electrophoresis (REA-PFGE; Giraffa et al, 2004; Herrero-Fresno et al, 2012), randomly amplified polymorphic DNA (RAPD; Ercolini et al, 2009; Franciosi et al, 2009; Bove et al, 2011), amplified fragment length polymorphism (AFLP; Lazzi et al, 2009), and repetitive extragenic palindromic-PCR (REP-PCR; Berthier et al, 2001; Bove et al, 2011; Mancini et al, 2012; Solieri et al, 2012). A different discriminatory power was found between these techniques.…”
Section: Molecular Approaches For Analyzing Raw-milk Long-ripened Chmentioning
confidence: 99%
“…In this study, the universal primer M13 was used to evaluate the diversity of isolated strains. This primer has been extensively used for biotyping LAB in dairy products by RAPD‐PCR by several authors (Gatti and others ; Rossetti and Giraffa ; De Dea Lindner and others ; Gomes and others ; Ruiz and others ; Albano and others ; Mancini and others ; Aponte and others ), and it was successfully used in this study to show the diversity between the species and to group the strains belonging to the same biotype (Randazzo and others ).…”
Section: Resultsmentioning
confidence: 99%
“…Cheeses containing microorganisms added to the coating were analyzed by the RAPD-PCR technique to verify the bacterial migration capacity of the surface into the cheese. The RAPD-PCR reveals the diversity and dynamics of some lactic acid bacteria strains, which have been useful to identify the presence of some microorganisms in complex environments such as cheese (Lazzi et al, 2009;Mancini, Lazzi, Bernini, Neviani, &Gatti, 2012;Randazzo, Caggia, & Neviani, 2009).According to Figure 7, that show some isolates obtained from the cheese interior (Lh, T3-AGLA and T4-AGLH) belong to the same species, since they are 100% similar. The similarity percentage between Lactobacillus acidophilus (La) and T3 (AGLA) strains was 20%, being considered low, not suggesting this microorganism migration into the cheese interior.…”
Section: Random Amplified Polymorphic Dna (Rapd)mentioning
confidence: 99%