Identification of Diagnostic Biomarkers for Compensatory Liver Cirrhosis Based on Gut Microbiota and Urine Metabolomics Analyses

Chen, Yingjun; Chen, Shaoxian; Xu, Chandi; Yu, Li; Chu, Shanshan; Bao, Jianzhi; Wang, Jinwei; Wang, Junwei

doi:10.1007/s12033-023-00922-9

Cited by 3 publications

(2 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To the best of our knowledge, Agathobacter has not been reported to be associated with osteoporosis. In a study identifying diagnostic biomarkers for compensatory liver cirrhosis, Agathobacter was elevated in patients with compensatory liver cirrhosis (Chen et al, 2023). In addition, previous studies have shown that Lactobacillus was more abundant in the osteoporosis group than in the control group (He et al, 2020), which was consistent with our results.…”

Section: Discussionsupporting

confidence: 92%

Table_1.xlsx

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 92%

Table_1.xlsx

View full text Add to dashboard Cite

“…To the best of our knowledge, Agathobacter has not been reported to be associated with osteoporosis. In a study identifying diagnostic biomarkers for compensatory liver cirrhosis, Agathobacter was elevated in patients with compensatory liver cirrhosis ( Chen et al., 2023 ). In addition, previous studies have shown that Lactobacillus was more abundant in the osteoporosis group than in the control group ( He et al., 2020 ), which was consistent with our results.…”

Section: Discussionmentioning

confidence: 99%

Characteristics of the gut microbiota and serum metabolites in postmenopausal women with reduced bone mineral density

Yan,

Wang,

et al. 2024

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

IntroductionEmerging evidence suggests that the gut microbiota is closely associated with bone homeostasis. However, little is known about the relationships among the bone mineral density (BMD) index, bone turnover markers, and the gut microbiota and its metabolites in postmenopausal women.MethodsIn this study, to understand gut microbiota signatures and serum metabolite changes in postmenopausal women with reduced BMD, postmenopausal individuals with normal or reduced BMD were recruited and divided into normal and OS groups. Feces and serum samples were collected for 16S rRNA gene sequencing, liquid chromatography coupled with mass spectrometry (LC-MS)-based metabolomics and integrated analysis.ResultsThe results demonstrated that bacterial richness and diversity were greater in the OS group than in the normal group. Additionally, distinguishing bacteria were found among the two groups and were closely associated with the BMD index and bone turnover markers. Metabolomic analysis revealed that the expression of serum metabolites, such as etiocholanolone, testosterone sulfate, and indole-3-pyruvic acid, and the corresponding signaling pathways, especially those involved in tryptophan metabolism, fatty acid degradation and steroid hormone biosynthesis, also changed significantly. Correlation analysis revealed positive associations between normal group-enriched Bacteroides abundance and normal group-enriched etiocholanolone and testosterone sulfate abundances; in particular, Bacteroides correlated positively with BMD. Importantly, the tryptophan-indole metabolism pathway was uniquely metabolized by the gut bacteria-derived tnaA gene, the predicted abundance of which was significantly greater in the normal group than in the control group, and the abundance of Bacteroides was strongly correlated with the tnaA gene.DiscussionOur results indicated a clear difference in the gut microbiota and serum metabolites of postmenopausal women. Specifically altered bacteria and derived metabolites were closely associated with the BMD index and bone turnover markers, indicating the potential of the gut microbiota and serum metabolites as modifiable factors and therapeutic targets for preventing osteoporosis.

show abstract

Chemical Investigation and Regulation of Adipogenic Differentiation of Cultivated Moringa oleifera

Le,

Kim,

Dang

et al. 2024

Pharmaceuticals

View full text Add to dashboard Cite

Background/Objectives: Moringa oleifera is a matrix plant with the high potential to cure several diseases with its medicinal and ethnopharmacological value and nutraceutical properties. In this study, we investigated the chemical and biological properties of this plant cultivated in our local region. Methods: Leaves, roots, seeds, stem bark, and twigs of oleifera were extracted and evaluated bioactivities targeting intracellular lipid accumulation and adipocyte differentiation in 3T3-L1 preadipocytes, and UHPLC-ESI-Orbitrap-MS/MS-Based molecular networking guided isolation and dereplication of metabolites from these extracts. Results: Five extracts of different organs of M. oleifera significantly stimulated intracellular lipid accumulation and adipocyte differentiation in 3T3-L1 preadipocytes in a concentration-dependent manner. These extracts markedly increased the expression of genes related to adipogenesis and lipogenesis. Notably, these extracts promoted peroxisome proliferator-activated receptor γ (PPARγ) activity and the expression of its target genes, including phosphoenolpyruvate carboxykinase, fatty acid-binding protein 4, and perilipin-2. These adipogenic and lipogenic effects of Moringa extracts through the regulation of PPARγ activity suggests their potential efficacy in preventing or treating type 2 diabetes. Furthermore, chemical investigation revealed high contents of phytonutrients as rich sources of secondary metabolites including glycosides, flavones, fatty acids, phenolics, and other compounds. In addition, in silico studies on major components of these extracts revealed the bioavailability of major components through their binding affinity to respective proteins targeting adipocyte differentiation.

show abstract

Identification of Diagnostic Biomarkers for Compensatory Liver Cirrhosis Based on Gut Microbiota and Urine Metabolomics Analyses

Cited by 3 publications

References 67 publications

Table_1.xlsx

Table_1.xlsx

Characteristics of the gut microbiota and serum metabolites in postmenopausal women with reduced bone mineral density

Chemical Investigation and Regulation of Adipogenic Differentiation of Cultivated Moringa oleifera

Contact Info

Product

Resources

About