Identification of differential duodenal gene expression levels and microbiota abundance correlated with differences in energy utilisation in chickens

Konsak, Barbara; Stanley, Dragana; Häring, Volker; Geier, Mark S.; Hughes, Robert J.; Howarth, Gordon S.; Crowley, Tamsyn M.; Moore, Robert J.

doi:10.1071/an12426

Cited by 21 publications

(13 citation statements)

References 23 publications

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“…When comparing the relative microbiota composition between RFI ranks, separately for females and males, only the Lactobacillus genus and two Lactobacillus crispatus -OTUs in feces were indicative for high RFI in females at both geographical locations. Moreover, the relative abundances of the two Lactobacillus crispatus -OTUs in cecal digesta and feces were also indicative for low TBWG and high FCR values, respectively, again only in females, thereby confirming previous findings [ 30 , 33 ]. Lactobacillus have been shown the ability to activate both innate defense mechanisms and adaptive immune responses in chicken’s intestine [ 29 , 36 , 37 ].…”

Section: Discussionsupporting

confidence: 87%

“…Because different selection strategies for FE (e.g. FCR, AME or RFI) were previously applied [ 5 , 7 , 8 , 30 ], this may have contributed to the inter-study variation in FE-associated bacterial taxa. This assumption is supported by findings in cattle where correlations between efficiency parameters and bacterial genera abundances differed depending on the FE metric used (i.e., FCR vs. RFI) [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

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Intestinal microbiota profiles associated with low and high residual feed intake in chickens across two geographical locations

et al. 2017

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Intestinal microbe-host interactions can affect the feed efficiency (FE) of chickens. As inconsistent findings for FE-associated bacterial taxa were reported across studies, the present objective was to identify whether bacterial profiles and predicted metabolic functions that were associated with residual feed intake (RFI) and performance traits in female and male chickens were consistent across two different geographical locations. At six weeks of life, the microbiota in ileal, cecal and fecal samples of low (n = 34) and high (n = 35) RFI chickens were investigated by sequencing the V3-5 region of the 16S rRNA gene. Location-associated differences in α-diversity and relative abundances of several phyla and genera were detected. RFI-associated bacterial abundances were found at the phylum and genus level, but differed among the three intestinal sites and between males and females. Correlation analysis confirmed that, of the taxonomically classifiable bacteria, Lactobacillus (5% relative abundance) and two Lactobacillus crispatus-OTUs in feces were indicative for high RFI in females (P < 0.05). In males, Ruminococcus in cecal digesta (3.1% relative abundance) and Dorea in feces (<0.1% relative abundance) were best indicative for low RFI, whereas Acinetobacter in feces (<1.5% relative abundance) related to high RFI (P < 0.05). Predicted metabolic functions in feces of males confirmed compositional relationships as functions related to amino acid, fatty acid and vitamin metabolism correlated with low RFI, whereas an increasing abundance of bacterial signaling and interaction (i.e. cellular antigens) genes correlated with high RFI (P < 0.05). In conclusion, RFI-associated bacterial profiles could be identified across different geographical locations. Results indicated that consortia of low-abundance taxa in the ileum, ceca and feces may play a role for FE in chickens, whereby only bacterial FE-associations found in ileal and cecal digesta may serve as useful targets for dietary strategies.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Intestinal microbiota profiles associated with low and high residual feed intake in chickens across two geographical locations

et al. 2017

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“…Moreover, L. salivarius and Lactobacillus agilis may enhance intestinal butyrate production, which may be beneficial for intestinal health via cross-feeding, as shown for an in vitro-simulated chicken cecum (22). Nevertheless, inconsistent findings were reported for the association between lactobacilli and FE in chickens (7,8,23,24), which may be due to particularities in experimental design, microbiota composition, diet, and the intestinal site investigated. When correlating individual RFI values with bacterial abundances in feces at 29 dph, the results indicated that RFI associations existed with other low-abundance phylotypes (relative abundances of 0.09 to 0.18% at 29 dph).…”

Section: Discussionmentioning

confidence: 99%

Fecal Microbiota Transplant from Highly Feed-Efficient Donors Shows Little Effect on Age-Related Changes in Feed-Efficiency-Associated Fecal Microbiota from Chickens

Siegerstetter

Petri

Magowan

et al. 2018

Appl Environ Microbiol

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Chickens with good or poor feed efficiency (FE) have been shown to differ in their intestinal microbiota composition. This study investigated differences in the fecal bacterial community of highly and poorly feed-efficient chickens at 16 and 29 days posthatch (dph) and evaluated whether a fecal microbiota transplant (FMT) from feed-efficient donors early in life can affect the fecal microbiota in chickens at 16 and 29 dph and chicken FE and nutrient retention at 4 weeks of age. A total of 110 chickens were inoculated with a FMT or a control transplant (CT) on dph 1, 6, and 9 and ranked according to residual feed intake (RFI; the metric for FE) on 30 dph. Fifty-six chickens across both inoculation groups were selected as the extremes in RFI (29 low, 27 high). RFI-related fecal bacterial profiles were discernible at 16 and 29 dph. In particular, ,, and operational taxonomic units were associated with low RFI (good FE). Multiple administrations of the FMT only slightly changed the fecal bacterial composition, which was supported by weighted UniFrac analysis, showing similar bacterial communities in the feces of both inoculation groups at 16 and 29 dph. Moreover, the FMT did not change the RFI and nutrient retention of highly and poorly feed-efficient recipients, whereas it tended to increase feed intake and body weight gain in female chickens. This finding suggests that host- and environment-related factors may more strongly affect chicken fecal microbiota and FE than the FMT. Modulating the chicken's early microbial colonization using a FMT from highly feed-efficient donor chickens may be a promising tool to establish a more desirable bacterial profile in recipient chickens, thereby improving host FE. Although FE-associated fecal bacterial profiles at 16 and 29 dph could be established, the microbiota composition of a FMT, when administered early in life, may not be a strong factor modulating the fecal microbiota at 2 to 4 weeks of life and reducing the variation in chicken's FE. Nevertheless, the present FMT may have potential benefits for growth performance in female chickens.

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“…Contrary to the latter two studies, bacterial phylotypes related to L. crispatus along with two other Lactobacillus species, were linked to low avian growth performance (Torok et al 2011). Also, Konsak et al (2013) found that an OTU which was most closely related to L.…”

Section: Most Of the Microbiota In Both Dietary Treatments Belonged Tmentioning

confidence: 99%

Peer Review #1 of "Sorghum and wheat differentially affect caecal microbiota and associated performance characteristics of meat chickens (v0.1)"

Vahjen¹

2017

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This study compared the effects of wheat-and sorghum-based diets on broiler chickens. The growth performance and caecal microbial community of chickens were measured and correlations between productivity and specific gut microbes were observed. Cobb broilers 15 days of age were individually caged and two dietary treatments were used, one with a wheat-based diet (n=48) and another one with a sorghum-based diet (n=48). Growth performance measurements were taken over a 10 day period and samples for microbiota analysis were taken at the end of that period. Caecal microbiota was characterised by sequencing of 16S bacterial rRNA gene amplicons. Overall, the results indicated that a sorghum-based diet produced higher apparent metabolisable energy (AME) and bodyweight gain (BWG) values in chickens, compared to a wheat-based diet. Nevertheless, sorghum-fed birds had higher feed conversion ratio (FCR) values than wheat-fed birds, possibly because of some anti-nutritional factors in sorghum. Further analyses showed that caecal microbial community was significantly associated with AME values, but microbiota composition differed between dietary treatments. A number of bacteria were individually correlated with growth performance measurements. Numerous OTUs assigned to strains of Lactobacillus crispatus and Lachnospiraceae, which were prevalent in sorghum-fed chickens, were correlated with high AME and BWG values, respectively. Additionally, a number of OTUs assigned to Clostridiales that were prevalent in wheat-fed chickens, were correlated with low FCR values. Overall, these results suggest that between-diet variations in growth performance were partly associated with changes in the caecal microbiota.

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Identification of differential duodenal gene expression levels and microbiota abundance correlated with differences in energy utilisation in chickens

Cited by 21 publications

References 23 publications

Intestinal microbiota profiles associated with low and high residual feed intake in chickens across two geographical locations

Intestinal microbiota profiles associated with low and high residual feed intake in chickens across two geographical locations

Fecal Microbiota Transplant from Highly Feed-Efficient Donors Shows Little Effect on Age-Related Changes in Feed-Efficiency-Associated Fecal Microbiota from Chickens

Peer Review #1 of "Sorghum and wheat differentially affect caecal microbiota and associated performance characteristics of meat chickens (v0.1)"

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