Identification of differential gene expression profile from peripheral blood cells of military pilots with hypertension by RNA sequencing analysis

Abstract

BackgroundElevated blood pressure is an important risk factor for cardiovascular disease and is also an important factor in global mortality. Military pilots are at high risk of cardiovascular disease because they undergo persistent noise, high mental tension, high altitude hypoxia, high acceleration and high calorie diet. Hypertension is the leading cause of cardiovascular disease in military pilots. In this study, we want to identify key genes from peripheral blood cells of military pilots with hypertension. Identification of these genes may help diagnose and control hypertension and extend flight career for military pilots.MethodsWe use RNA sequencing technology, bioinformatics analysis and Western blotting to identify key genes from peripheral blood cells of military pilots with hypertension.ResultsOur study detected 121 up-regulated genes and 623 down-regulated genes in the peripheral blood mononuclear cells (PBMCs) from hypertensive military pilots. We have also identified 8 important genes (NME4, PNPLA7, GGT5, PTGS2, IGF1R, NT5C2, ENTPD1 and PTEN), a number of gene ontology categories and biological pathways that may be associated with military pilot hypertension.ConclusionsOur study may provide effective means for the prevention, diagnosis and treatment of hypertension for military pilot and extend their flight career.Electronic supplementary materialThe online version of this article (10.1186/s12920-018-0378-2) contains supplementary material, which is available to authorized users.

Expanding the clinical relevance of the 5′-nucleotidase cN-II/NT5C2

“…Also, [16] 3 members of consanguineous family L460P [17] rs11191593 was associated with blood pressure [35]. Finally, a gene expression study showed that military pilots with hypertension had a lower cN-II expression than control pilots [36]. Interestingly, there was also a decreased expression of CD39/ENTPD1, confirming an additional link between these two proteins after the one in HSP.…”

Structural variants in the Chinese population and their impact on phenotypes, diseases and population adaptation

“…In addition, a PBS signal with a 1.4 kb INS was located in the 13th intron of patatin like phospholipase domain containing 7 ( PNPLA7 ). A previous study reported that PNPLA7 was associated with hypertension based on RNA sequencing analysis 39 . Blood pressure and the prevalence of high blood pressure are greater in the northern than the southern Chinese population 40 .…”

<b><i>NT5C2</i></b> Gene Polymorphisms and the Risk of Coronary Heart Disease

“…Additionally, CHD is associated with hypertension in both diabetic and nondiabetic populations [33]. The morbidity and mortality of CVD are related to degrees of increased blood pressure [34]. This suggests that CHD has a close relationship with hypertension and diabetes.…”