Identification of differentially expressed genes in T‐lymphoid malignancies in an animal model system

Starkey, Cindi R.; Lévy, Laurence

doi:10.1002/ijc.2910620316

Cited by 33 publications

(27 citation statements)

References 21 publications

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“…There are reports that expression of NADH dehydrogenase or cytochrome c oxidase is up-regulated in several kinds of cancers. 63,64 Our result was the opposite. Of note is the fact that human RCCs show a high incidence of mitochondrial DNA deletion.…”

Section: Discussioncontrasting

confidence: 53%

Expression of Stress-Response and Cell Proliferation Genes in Renal Cell Carcinoma Induced by Oxidative Stress

Tanaka

Kondo

Iwasa

et al. 2000

The American Journal of Pathology

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Section: Discussioncontrasting

confidence: 53%

Expression of Stress-Response and Cell Proliferation Genes in Renal Cell Carcinoma Induced by Oxidative Stress

Tanaka

Kondo

Iwasa

et al. 2000

The American Journal of Pathology

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“…Several aminoacyl-tRNA synthetases were identified here (spots 5, 8, 9, 23, 85), as were various isoforms of large and small ribosomal subunits (spots 75, 86 to 91, 93). These ribosomal proteins may function to anchor ribosomes to MTs (51) or may have functions that extend beyond translation (52).…”

Section: Resultsmentioning

confidence: 99%

Large-scale Identification of Tubulin-binding Proteins Provides Insight on Subcellular Trafficking, Metabolic Channeling, and Signaling in Plant Cells

Chuong

Good

Taylor

et al. 2004

Molecular & Cellular Proteomics

115

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Microtubules play an essential role in the growth and development of plants and are known to be involved in regulating many cellular processes ranging from translation to signaling. In this article, we describe the proteomic characterization of Arabidopsis tubulin-binding proteins that were purified using tubulin affinity chromatography. Microtubule co-sedimentation assays indicated that most, if not all, of the proteins in the tubulin-binding protein fraction possessed microtubule-binding activity. Two-dimensional gel electrophoresis of the tubulin-binding protein fraction was performed, and 86 protein spots were excised and analyzed for protein identification. A total of 122 proteins were identified with high confidence using LC-MS/MS. These proteins were grouped into six categories based on their predicted functions: microtubule-associated proteins, translation factors, RNA-binding proteins, signaling proteins, metabolic enzymes, and proteins with other functions. Almost one-half of the proteins identified in this fraction were related to proteins that have previously been reported to interact with microtubules. This study represents the first large-scale proteomic identification of eukaryotic cytoskeleton-binding proteins, and provides insight on subcellular trafficking, metabolic channeling, and signaling in plant cells. Molecular & Cellular Proteomics 3:970 -983, 2004.The cytoskeleton is the single most important structure that contributes to the highly ordered organization of the eukaryotic cell. It provides a framework for cell division and the trafficking of organelles and macromolecules, and also serves to regulate important cellular processes such as signaling, translation, and metabolism. The cytoskeleton plays a key role in a number of plant-specific processes, such as assisting in the formation of the cell plate, regulating cell-to-cell movement, and influencing the direction of cell elongation (1). A role for the microtubule (MT) 1 component of the cytoskeleton in many of these processes has been demonstrated, and a number of MT-binding proteins that are responsible for regulating these events have been identified.Plant MTs are assembled into four distinct arrays during the cell cycle (2). Three of these arrays-the interphase cortical array, the pre-prophase band, and the phragmoplast-have no counterpart in animal cells. The cortical MT array has been linked to the regulation of cellulose microfibril deposition and, hence, a role in cell expansion, while the pre-prophase band and the phragmoplast have important roles in the positioning and synthesis of the new cell plate in dividing cells. The fourth array, the spindle, has an evolutionarily conserved role in the segregation of chromosomes during cell division. The organization and dynamics of MTs in these arrays depend on the activity of various MT-associated proteins (MAPs). Several plant MAPs have been identified, including the 65-kDa MAPs, MAP 190, and MOR1 (3). These proteins are important in cross-bridging MTs, linking MTs with actin filamen...

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“…In the human promyelocytic leukemia cell line HL-60, antisense sequences of the FTE/S3a gene have been reported to induce apoptosis (42). In feline thymic tumor, FTE/S3a steady state mRNA levels were 2.7-fold higher than in normal thymus (43). Overexpression of the FTE/S3a gene was also found in surgical specimens of both primary tumors and metastatic foci of medullary thyroid carcinomas and in a panel of recurrent and metastatic medullary thyroid carcinoma, when compared with normal thyroid tissue and normal lymphatic tissue.…”

Section: Discussionmentioning

confidence: 97%

Novel Interaction between the Transcription Factor CHOP (GADD153) and the Ribosomal Protein FTE/S3a Modulates Erythropoiesis

Cui¹,

Coutts²,

Stahl³

et al. 2000

Journal of Biological Chemistry

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The transcription factor CHOP (GADD153) heterodimerizes with other C/EBP family members, especially C/EBP␤, thus preventing their homodimerization and binding to DNA sequences specific for the homodimers. Some CHOP-C/EBP heterodimers apparently bind to alternative DNA sequence and thereby regulate the transcription of other genes. Recently, we demonstrated that CHOP is up-regulated during certain stages of erythroid differentiation and that ectopic overexpression of CHOP enhances this process (Coutts, M., Cui, K., Davis, K. L., Keutzer, J. C., and Sytkowski, A. J. (1999) Blood 93, 3369 -3378). In the present study, we report that CHOP also interacts with another non-C/EBP protein designated v-fos transformation effector (FTE) (Kho, C. J., and Zarbl, H. The growth of erythroid progenitor cells in the bone marrow and their differentiation into enucleate, hemoglobinized erythrocytes is regulated primarily by the glycoprotein hormone erythropoietin (Epo) 1 (1). This growth factor interacts with its cognate receptor on the surface of the erythroid cell and triggers a signal transduction cascade that results in cell proliferation (anti-apoptosis) and differentiation (2-13).A role in erythroid growth and development has been shown or suggested for several regulatory proteins including Myc, Myb, GATA-1, and NF-E2 (4, 7, 8, 14 -19). Recently, we found that Epo up-regulates the expression of CHOP (gadd153) (20 -24), a member of the C/EBP family of transcription factors (25). Gain-of-function studies indicated that increasing CHOP expression enhances hemoglobinization of erythroid cells, indicating a functional role for CHOP in erythroid differentiation. Additionally, we obtained evidence that CHOP protein can bind to several nuclear proteins from erythroid cells, potentially some that are not C/EBP family members.We have now used the yeast two-hybrid system (26) to screen an erythroid cell cDNA library for proteins that interact with CHOP. We report that CHOP interacts with a non-C/EBP protein designated v-fos transformation effector (FTE) (27) which is identical to ribosomal protein S3a (28). Our results indicate that this interaction inhibits the ability of CHOP to enhance erythroid differentiation. EXPERIMENTAL PROCEDURESRauscher Murine Erythroleukemia Cell cDNA Library Construction and Yeast Two-hybrid Screen-A library from Rauscher murine erythroleukemia cells (29, 30) was constructed into the pAD-Gal4 vector. Briefly, 5 g of poly(A) ϩ RNA was converted to double-stranded DNA using Stratascript RNase H Ϫ reverse transcriptase (Stratagene). First strand synthesis was primed with an oligonucleotide containing poly(dT) and an XhoI site (31). Second strand synthesis was carried out using Escherichia coli DNA polymerase I and RNase H (32). The cDNA ends were filled with T4 DNA polymerase, and the internal EcoRI sites of the cDNA were methylated with EcoRI methylase. Following EcoRI linker addition and size fractionation in a 1% agarose gel, doublestranded cDNA over 800 base pairs was cleaved with EcoRI and XhoI. The fragm...

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Identification of differentially expressed genes in T‐lymphoid malignancies in an animal model system

Cited by 33 publications

References 21 publications

Expression of Stress-Response and Cell Proliferation Genes in Renal Cell Carcinoma Induced by Oxidative Stress

Expression of Stress-Response and Cell Proliferation Genes in Renal Cell Carcinoma Induced by Oxidative Stress

Large-scale Identification of Tubulin-binding Proteins Provides Insight on Subcellular Trafficking, Metabolic Channeling, and Signaling in Plant Cells

Novel Interaction between the Transcription Factor CHOP (GADD153) and the Ribosomal Protein FTE/S3a Modulates Erythropoiesis

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