Identification of Differentially Expressed Genes by cDNA-AFLP Technique in Response to Drought Stress in Triticum durum

Melloul, Marouane; Iraqi, Driss; Alaoui, MyAbdelaziz El; Erba, Gilles; Alaoui, Sanaa; Ibriz, Mohammed; Fahime, Elmostafa El

doi:10.17113/ftb.52.04.14.3701

Cited by 11 publications

(3 citation statements)

References 76 publications

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“…In agreement, research reports elucidated that cytochrome P450 family protein involves in drought and salinity stresses (Narusaka et al, 2004 ; Ehlting et al, 2008 ; Jun et al, 2015 ). Particularly, Melloul et al ( 2014 ) showed the upregulation of cytochrome P450 proteins in durum wheat leaves under drought stress. Another MTA was identified associated with DGF-TOL on chromosome 4A (IAAV1775) at 590,188,609 bp, annotated as a methyltransferase.…”

Section: Discussionmentioning

confidence: 99%

Association Mapping of Drought Tolerance Indices in Ethiopian Durum Wheat (Triticum turgidum ssp. durum)

et al. 2022

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Ethiopia is a major producer of durum wheat in sub-Saharan Africa. However, its production is prone to drought stress as it is fully dependent on rain, which is erratic and unpredictable. This study aimed to detect marker-trait associations (MTAs) and quantitative trait loci (QTLs) related to indices. Six drought tolerance indices, i.e., drought susceptibility index (DSI), geometric mean productivity (GMP), relative drought index (RDI), stress tolerance index (STI), tolerance index (TOL), and yield stability index (YSI) were calculated from least-square means (lsmeans) of grain yield (GY) and traits significantly (p < 0.001) correlated with grain yield (GY) under field drought stress (FDS) and field non-stress (FNS) conditions. GY, days to grain filling (DGF), soil plant analysis development (SPAD) chlorophyll meter, seeds per spike (SPS), harvest index (HI), and thousand kernel weight (TKW) were used to calculate DSI, GMP, RDI, STI, TOL, and YSI drought indices. Accessions, DW084, DW082, DZ004, C037, and DW092 were selected as the top five drought-tolerant based on DSI, RDI, TOL, and YSI combined ranking. Similarly, C010, DW033, DW080, DW124-2, and C011 were selected as stable accessions based on GMP and STI combined ranking. A total of 184 MTAs were detected linked with drought indices at –log10p ≥ 4.0,79 of which were significant at a false discovery rate (FDR) of 5%. Based on the linkage disequilibrium (LD, r2 ≥ 0.2), six of the MTAs with a positive effect on GY-GMP were detected on chromosomes 2B, 3B, 4A, 5B, and 6B, explaining 14.72, 10.07, 26.61, 21.16, 21.91, and 22.21% of the phenotypic variance, respectively. The 184 MTAs were clustered into 102 QTLs. Chromosomes 1A, 2B, and 7A are QTL hotspots with 11 QTLs each. These chromosomes play a key role in drought tolerance and respective QTL may be exploited by marker-assisted selection for improving drought stress tolerance in wheat.

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Section: Discussionmentioning

confidence: 99%

Association Mapping of Drought Tolerance Indices in Ethiopian Durum Wheat (Triticum turgidum ssp. durum)

et al. 2022

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“…Finally, among differentially expressed transcripts in potato adapted cells we noticed an impressive number of genes involved in defence to pathogens, which may correlate with the ethylene biological activity to prompt biotic cell response (Ecker and Davis, 1987). Interestingly, some of these genes have already been linked to abiotic stress responses such as mildew resistance locus (MLO), disease resistance protein 2 (RGA 2) and syntaxin 121 (SYP121) (Hachez et al, 2014;Melloul et al, 2014;Piffanelli et al, 2002).…”

Section: Modulation Of Ethylene Signalling Network As a Distinctive Tmentioning

confidence: 99%

Distinct gene networks drive differential response to abrupt or gradual water deficit in potato

Ambrosone

Batelli

Bostan

et al. 2017

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Water-limiting conditions affect dramatically plant growth and development and, ultimately, yield of potato plants (Solanum tuberosum L.). Therefore, understanding the mechanisms underlying the response to water deficit is of paramount interest to obtain drought tolerant potato varieties. Herein, potato 10K cDNA array slides were used to profile transcriptomic changes of two potato cell populations under abrupt (shocked cells) or gradual exposure (adapted cells) to polyethylene glycol (PEG)-mediated water stress. Data analysis identified >1000 differentially expressed genes (DEGs) in our experimental conditions. Noteworthy, our microarray study also suggests that distinct gene networks underlie the cellular response to shock or gradual water stress. On the basis of our experimental findings, it is possible to speculate that DEGs identified in shocked cells participate in early protective and sensing mechanisms to environmental insults, while the genes whose expression was modulated in adapted cells are directly involved in the acquisition of a new cellular homeostasis to cope with water stress conditions. To validate microarray data obtained for potato cells, the expression analysis of 21 selected genes of interest was performed by Real-Time Quantitative Reverse Transcription PCR (qRT-PCR). Intriguingly, the expression levels of these transcripts in 4-week old potato plants exposed to long-term water-deficit. qRT-PCR analysis showed that several genes were regulated similarly in potato cells cultures and tissues exposed to drought, thus confirming the efficacy of our simple experimental system to capture important genes involved in osmotic stress response. Highlighting the differences in gene expression between shock-like and adaptive response, our findings could contribute to the discussion on the biological function of distinct gene networks involved in the response to abrupt and gradual adaptation to water deficit.

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“…Various types of DNA markers have been developed and are successfully used to study the genetic diversity of accessions of the genus Triticum L. (Röder et al, 1998;Song et al, 2005;Singh et al, 2018). PCR-based markers, such as RAPD, AFLP, and SSR, are widely used tools for studying genetic diversity and discrimination both durum and common wheat (Khlestkina et al, 2002;Kudriavtsev et al, 2004;Yildirim et al, 2011;Abugalieva et al, 2012;Melloul et al, 2014;Adonina et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Phenotypic and genetic variability of a tetraploid wheat collection grown in Kazakhstan

et al. 2020

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New cultivars adapted to major durum wheat growing environments are essential for the cultivation of this crop. The development of new cultivars has required the availability of diverse genetic material and their extensive field trials. In this work, a collection of tetraploid wheat consisting of 85 accessions was tested in the field conditions of Almaty region during 2018 and 2019. The accessions were ranged according to nine agronomic traits studied, and accessions with the highest yield performance for Almaty region of Kazakhstan were revealed. The ANOVA suggested that the performance of agronomic traits were influenced both by Environment and Genotype. Also, the collection was analyzed using seven SSR (simple sequence repeats) markers. From 3 to 6 alleles per locus were revealed, with an average of 4.6, while the effective number of alleles was 2.8. Nei’s genetic diversity was in the range of 0.45–0.69. The results showed high values of polymorphism index content (PIC) in the range of 0.46–0.70, with an average of 0.62, suggesting that 6 out of 7 SSRs were highly informative (PIC > 0.5). Phylogenetic analysis of the collection has allowed the separation of accessions into six clusters. The local accessions were presented in all six clusters with the majority of them grouped in the first three clusters designated as A, B, and C, respectively. The relations between SSR markers and agronomic traits in the collection were studied. The results can be efficiently used for the enhancement of local breeding projects for the improvement of yield productivity in durum wheat.

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Identification of Differentially Expressed Genes by cDNA-AFLP Technique in Response to Drought Stress in Triticum durum

Cited by 11 publications

References 76 publications

Association Mapping of Drought Tolerance Indices in Ethiopian Durum Wheat (Triticum turgidum ssp. durum)

Association Mapping of Drought Tolerance Indices in Ethiopian Durum Wheat (Triticum turgidum ssp. durum)

Distinct gene networks drive differential response to abrupt or gradual water deficit in potato

Phenotypic and genetic variability of a tetraploid wheat collection grown in Kazakhstan

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