Identification of differentially expressed genes in tolerant and susceptible potato cultivars in response to Spongospora subterranea f. sp. subterranea tuber infection

Abstract

Powdery scab caused by Spongospora subterranea f. sp. subterranea (Sss) has recently become one of the most devastating potato diseases of economic importance in South Africa. The use of resistant cultivars has long been considered the most effective and sustainable strategy to manage the pathogen. However, little is known about the molecular mechanisms underlying resistance of potato tubers to Sss. Using RNA-sequencing (RNA-seq), 2058 differentially expressed genes (DEGs) were identified from two potato cultivars (tolerant and susceptible) in response to Sss infection. Analysis of the expression patterns of 10 selected defence-response genes was carried out at two different stages of tuber growth using RT-qPCR to validate the RNA-seq data. Several defence-related genes showed contrasting expression patterns between the tolerant and susceptible cultivars, including marker genes involved in the salicylic acid hormonal response pathway (StMRNA, StUDP and StWRKY6). Induction of six defence-related genes (StWRKY6, StTOSB, StSN2, StLOX, StUDP and StSN1) persisted until harvest of the tubers, while three other genes (StNBS, StMRNA and StPRF) were highly up-regulated during the initial stages of disease development. The results of this preliminary study suggest that the tolerant potato cultivar employs quantitative resistance and salicylic acid pathway hormonal responses against tuber infection by Sss. The identified genes have the potential to be used in the development of molecular markers for selection of powdery scab resistant potato lines in marker-assisted breeding programmes.

Spore Germination of the Obligate Biotroph Spongospora subterranea: Transcriptome Analysis Reveals Germination Associated Genes

“…In total, 9,730 and 12,732 (7,490 were full-length) proteins were predicted for P. brassicae and S. subterranea, respectively (Schwelm et al, 2015). Lekota et al (2019) analysed the responses of susceptible and resistant potato cultivars to S. subterranea infection and identified several defence−response genes that showed contrasting expression patterns between the susceptible and resistant cultivars. There are several other examples of the interaction between plasmodiophorids and their hosts (Barr et al, 1995;Zhao et al, 2017;Galindo-González et al, 2020;Ji et al, 2021).…”

Dynamic Transcriptome Profiling of Mungbean Genotypes Unveil the Genes Respond to the Infection of Mungbean Yellow Mosaic Virus

“…Despite VGGRU-1 and VRM (Gg) 1 having different MYMV infection responses, 275 common DEGs were found in both mungbean genotypes. Previous studies have shown the advantage of focusing on the common DEGs between the resistant and susceptible genotypes to discover the possible candidate resistance genes in various diseases [15,22]. Therefore, we were mainly focused on 275 common DEGs.…”

“…Taking advantage of second and third-generation sequencing technologies, comparative transcriptome analysis through RNA sequencing (RNA seq) is the most popular method for detecting DEGs between two models [10][11][12][13]. Moreover, transcriptome analysis provides detailed information to understand the dynamics of interaction between the host and pathogen [14,15]. By comparing the transcriptome data from the pathogen-infected and control, countless studies have been attempted to elucidate the complete details of the genes and pathways involved in the molecular mechanism of resistance to pathogens [16][17][18].…”

Reacción de genotipos de papa “criolla” (Solanum tuberosum grupo phureja) al inoculo natural de Rhizoctonia solani Kühn

“…Existe gran variabilidad genética de papa criolla (S. tuberosum grupo phureja) de buena aceptación en el mercado, por presentar características deseables para la culinaria e industria así mismo este grupo presenta mayor cantidad de proteína en comparación al grupo tuberosum, también buenos contenidos Fe, Zn y antioxidantes como vitamina C y fenoles (Ñustez y Rodríguez, 2020). Por otra parte, se han encontrado materiales con tolerancia a diferentes enfermedades como: tizón tardío (Phytophthora infestans) (Monteros y Delgado, 2021), virus del amarillamiento de venas (Torrance et al, 2020), marchitez bacteriana (Ralstonia solanacearum) (Lopes et al, 2021), sarna polvosa (Spongospora subterranea) (Lekota et al, 2019) y costra negra (Rhizoctonia solani) (Zhang et al, 2021). De ahí que estas características del grupo phureja constituyen el recurso genético para futuras investigaciones.…”