Identification of differentially expressed genes in hip cartilage with femoral head necrosis, based on genome‑wide expression profiles

Abstract

Necrosis of the femoral head (NFH), a severe orthopedic disease in adults, involves the collapse of the femoral head. The pathophysiological mechanisms underlying NFH are yet to be fully investigated. The aim of the present study was to identify potentially important genes and signaling pathways involved in NFH and investigate their molecular mechanisms. Gene expression profiles of patients with NFH and healthy controls were compared using the Gene Expression Omnibus (GEO) database repository of the National Center of Biotechnology Information. GSE74089 from the GEO database included 4 patients with NFH and 4 healthy individuals. A total of 1,191 differentially expressed genes (DEGs) were identified between the patients with NFH and controls, including 743 upregulated and 448 downregulated DEGs. Then, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis revealed that upregulated DEGs were mainly involved in the phosphoinositide 3-kinase/protein kinase B signaling pathway, focal adhesion and extracellular matrix-receptor interactions. Additionally, protein-protein interaction (PPI) analysis identified the most central DEGs as vascular endothelial growth factor A, Jun proto-oncogene, cyclin D1, fibroblast growth factor 2, HECT domain and ankyrin repeat-containing E3 ubiquitin protein ligase 1, protein kinase Cα, bone morphogenetic protein 2 and prostaglandin-endoperoxide synthase 2. PPI analysis also identified guanine nucleotide-binding protein, γ13 as the most commonly downregulated gene based on different centrality. The results of the present study may provide novel insight into the genes and associated pathways involved in NFH, and aid the identification of novel therapeutic targets and biomarkers in the treatment of NFH.

Exploring molecular mechanisms of intra-articular changes in osteonecrosis of femoral head using DIA proteomics and bioinformatics

“…Focal adhesion, an important structure of the cell skeleton, may be involved in cell growth regulation at the early stages of osteonecrosis [ 26 , 27 ]. The digestion and absorption of proteins may be related to bone balance disruption and articular cartilage collapse at the late stages of osteonecrosis [ 28 ].…”

Analysis of Differentially Expressed Genes in Osteonecrosis of Femoral Head, Osteoarthritis, and Rheumatoid Arthritis by Integrated Microarray Analysis

“…Based on the above information analysis, in the initial stage of ONFH, reduced angiogenesis, insu cient blood supply, hypoxia, metabolic disorders, leading to local osteonecrosis, apoptosis, osteoclastmediated bone resorption [20,32,33] . Then the osteoblast-mediated repair response begins [34,35] . However, the imbalance between bone remodeling and resorption destruction leads to structural damage and collapse of the subchondral bone of the femoral head.…”