Identification of differentially expressed microRNAs during preadipocyte differentiation in Chinese crested duck

Wang, Shasha; Zhang, Yang; Yuan, Xi; Pan, Rui; Yao, Wencheng; Zhong, Li; Song, Qianqian; Zheng, Shengping; Wang, Zhixiu; Xu, Qiang; Chang, Guobin; Chen, Guohong

doi:10.1016/j.gene.2018.03.085

Cited by 12 publications

(9 citation statements)

References 29 publications

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“…Numerous studies have shown that intramuscular fat not only correlated with muscle flavor, but also related to many other meat quality traits (such as muscle pH, hydraulics, tenderness, etc.) [2,3,4,5,6]. Our previous studies have found that there were significant differences in the density and size of lipid droplets in the breast muscle between the juvenile and late-laying period of Gushi hens.…”

Section: Introductionmentioning

confidence: 99%

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gga-miRNA-18b-3p Inhibits Intramuscular Adipocytes Differentiation in Chicken by Targeting the ACOT13 Gene

Sun

et al. 2019

Cells

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Intramuscular fat (IMF) is the most important evaluating indicator of chicken meat quality, the content of which is positively correlated with tenderness, flavor, and succulence of the meat. Chicken IMF deposition process is regulated by many factors, including genetic, nutrition, and environment. Although large number of omics’ studies focused on the IMF deposition process, the molecular mechanism of chicken IMF deposition is still poorly understood. In order to study the role of miRNAs in chicken intramuscular adipogenesis, the intramuscular adipocyte differentiation model (IMF-preadipocytes and IMF-adipocytes) was established and subject to miRNA-Seq. A total of 117 differentially expressed miRNAs between two groups were obtained. Target genes prediction and functional enrichment analysis revealed that eight pathways involved in lipid metabolism related processes, such as fatty acid metabolism and fatty acid elongation. Meanwhile a putative miRNA, gga-miR-18b-3p, was identified be served a function in the intramuscular adipocyte differentiation. Luciferase assay suggested that the gga-miR-18b-3p targeted to the 3′UTR of ACOT13. Subsequent functional experiments demonstrated that gga-miR-18b-3p acted as an inhibitor of intramuscular adipocyte differentiation by targeting ACOT13. Our findings laid a new theoretical foundation for the study of lipid metabolism, and also provided a potential target to improve the meat quality in the poultry industry.

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Section: Introductionmentioning

confidence: 99%

“…Intramuscular fat mainly consisted of intramuscular adipocytes, which were differentiated by intramuscular preadipocytes. In recent years, many previous studies focused on intramuscular adipocytes in various species, such as cattle and pig [5,6]. Seldom research performed on chicken.…”

Section: Introductionmentioning

confidence: 99%

gga-miRNA-18b-3p Inhibits Intramuscular Adipocytes Differentiation in Chicken by Targeting the ACOT13 Gene

Sun

et al. 2019

Cells

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“…In hepatocellular carcinoma (HCC) 15 circRNAs, 17 miRNAs, and 89 mRNAs were found to be correlated in ceRNA network [ 29 ]. Previous studies have explored the expression profiles of non-coding RNAs (ncRNAs) including miRNAs and long non-coding RNAs (lncRNAs) in poultry [ 30 , 31 ], however, the specific function of circRNAs in duck adipocytes is still unknown.…”

Section: Discussionmentioning

confidence: 99%

Circular RNA expression profiling reveals that circ-PLXNA1 functions in duck adipocyte differentiation

et al. 2020

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Adipocytes are derived from pluripotent mesenchymal stem cells through adipogenesis. Pre-adipocyte differentiation in poultry greatly influences fat deposition and meat quality. Circular RNAs (circRNAs) have an important function in cancer and some differentiation processes. Herein, high-throughput transcriptome sequencing was used to detect circRNAs present in cherry valley duck pre-adipocyte and adipocyte differentiation over 3 days. We identified 9,311 circRNAs and 141 differentially expressed circRNAs. Sequencing results were verified through qRT-PCR using seven randomly selected circRNAs, and competing endogenous RNA (ceRNA) networks were exhibited by ten important circRNAs in duck adipocyte differentiation. circRNA plexin A1 (circ-PLXNA1) was detected in duck adipocytes and mainly expressed in adipose, leg muscle and liver. Inhibition of circ-PLXNA1 limited the differentiation of duck adipocyte. There were four corresponding miRNAs for circ-PLXNA1 and 313 target genes for those miRNAs. CeRNA“circ-PLXNA1/miR-214/CTNNB1 axis” was focused and verified by a dual-luciferase reporter experiment. After co-transfection of cells with si-circ-PLXNA1 and miR-214 mimics, the expression level of CTNNB1 was down-regulated, triglyceride content and the adipogenic capacity of preadipocytes decreased. While there were no significant change after si-CTNNB1 transfection. All these results provide further insight into the circRNAs, especially for circ-PLXNA1 in duck adipocyte differentiation.

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“…Similarly, using HiSeq sequencing, Wang and colleagues(Wang et al 2017) identified 329 known miRNAs and 157 new miRNAs during the development of porcine adipose. Additionally, Wang and coworkers(Wang et al 2018b) identified 105 DE miRNAs through the deep sequencing of duck adipose tissue and differentiated proadipocytes in vitro , demonstrating that miRNA expression varies among different species.…”

Section: Discussionmentioning

confidence: 99%

“…Gu and colleagues (Gu and Eleswarapu 2007) screened miRNAs in bovine adipose tissue and breast tissue and identified 59 DE miRNAs, 5 of which differed from known mammalian miRNAs. Wang et al(Wang et al 2018b) constructed an in vitro adipogenesis model of Crest-feather ducks and performed deep miRNA-sequencing, identifying 105 DE miRNAs, 12 of which were newly predicted and related to adipogenesis, including miR-223, miR-184-3p and miR-10b-5.…”

Section: Introductionmentioning

confidence: 99%

Screening and identification of MicroRNAs expressed in perirenal adipose tissue during rabbit growth

Wang

Guo

Tian

et al. 2019

Preprint

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45MiRNAs regulate adipose tissue development, which are closely 46 related to subcutaneous and intramuscular fat deposition and adipocyte 47 differentiation. As an important economic and agricultural animal, rabbits 48 have low adipose tissue deposition and are an ideal model to study 49 adipose regulation. However, the miRNAs related to fat deposition during 50 the growth and development of rabbits are poorly defined. In this study, 51 miRNA-sequencing and bioinformatics analyses were used to profile the 52 miRNAs in rabbit perirenal adipose tissue at 35, 85 and 120 days 53 post-birth. Differentially expressed (DE) miRNAs between different 54 stages were identified by DEseq in R. Target genes of DE miRNAs were 55 predicted by TargetScan and miRanda. To explore the functions of 56 identified miRNAs, Gene Ontology (GO) enrichment and Kyoto 57 Encyclopedia of Genes and Genomes (KEGG) pathway analyses were 58 performed. Approximately 1.6 GB of data was obtained by miRNA-seq. 59 A total of 987 miRNAs (780 known and 207 newly predicted) and 174 60 DE miRNAs were identified. The miRNAs ranged from 18nt to 26nt. GO 61 enrichment and KEGG pathway analyses revealed that the target genes of 62 the DE miRNAs were mainly involved in zinc ion binding, regulation of 63 cell growth, MAPK signaling pathway, and other adipose 64 hypertrophy-related pathways. Six DE miRNAs were randomly selected 65 and their expression profiles were validated by q-PCR. In summary, we 66 4 provide the first report of the miRNA profiles of rabbit adipose tissue 67 during different growth stages. Our data provide a theoretical reference 68 for subsequent studies on rabbit genetics, breeding and the regulatory 69 mechanisms of adipose development.70 Introduction 71 MicroRNAs (miRNAs) are endogenous non-coding RNAs, typically 72 18～26 nucleotides in length, that regulate gene expression in eukaryotic 73 cells. Mature miRNAs are produced from long primary transcripts 74 through a series of nucleases that are further assembled into 75 RNA-induced silencing complexes. These complexes recognize target 76 mRNAs by complementary base pairing, leading to mRNA degradation 77 and the inhibition of translation(Fabian et al. 2010). MiRNAs regulate a 78 wide range of physiological processes, including growth and 79 development, virus defense, cell proliferation, apoptosis and fat 80 metabolism. Meanwhile, it has been well documented that MiRNAs 81 regulate adipose tissue development, which are closely related to 82 subcutaneous and intramuscular fat deposition(Guoxi et al. 2011; Guo et 83 al. 2012) and adipocyte differentiation(Son et al. 2014).MiRNAs, 84 including miR-27b(Karbiener et al. 2009), miR-103(Meihang et al. 2015) 85 and miR-148a(Shi et al. 2015) regulate adipogenic processes, promoting 86 or inhibiting adipogenesis in animals. This implicates miRNAs can be a 87 5 new target for studying the molecular mechanisms governing fat 88 development, growth and deposition in animals. 89 To-date, studies on the role of miRNAs during fat development have 90 ...

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Identification of differentially expressed microRNAs during preadipocyte differentiation in Chinese crested duck

Cited by 12 publications

References 29 publications

gga-miRNA-18b-3p Inhibits Intramuscular Adipocytes Differentiation in Chicken by Targeting the ACOT13 Gene

gga-miRNA-18b-3p Inhibits Intramuscular Adipocytes Differentiation in Chicken by Targeting the ACOT13 Gene

Circular RNA expression profiling reveals that circ-PLXNA1 functions in duck adipocyte differentiation

Screening and identification of MicroRNAs expressed in perirenal adipose tissue during rabbit growth

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