Identification of differentially expressed microRNAs between Bacillus thuringiensis Cry1Ab-resistant and -susceptible strains of Ostrinia furnacalis

Xu, Lei; Ling, Yinghui; Wang, Yueqin; Wang, Zhenying; Hu, B.; Zhou, Zhongze; Hu, Fei; He, Kanglai

doi:10.1038/srep15461

Cited by 31 publications

(46 citation statements)

References 49 publications

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“…MiRNA plays very important roles in the growth and development of insects, such as the reproductive process38 germ cell development39 neurogenesis4041, wing development424344, phenotypic plasticity45 and muscle growth464748. More recently, miRNAs were also found to be involved in insecticide resistance495051.…”

Section: Discussionmentioning

confidence: 99%

“…The unique differentially expressed miRNAs in the ZZ strain, especially those that have different expression profiles compared with CHR, are more likely to be involved in other insecticide resistance and not chlorantraniliprole resistance. Some of them have been reported to be associated with deltamethrin, Cry1Ab or fenpropathrin resistance, such as miR-210, miR-965, miR-981, miR-1, miR-306 and miR-281495051.…”

Section: Discussionmentioning

confidence: 99%

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Global identification of microRNAs associated with chlorantraniliprole resistance in diamondback moth Plutella xylostella (L.)

Zhu

Liu

et al. 2017

Sci Rep

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The diamondback moth (DBM), Plutella xylostella (L.), is one of the most serious cruciferous pests and has developed high resistance to most insecticides, including chlorantraniliprole. Previous studies have reported several protein-coding genes that involved in chlorantraniliprole resistance, but research on resistance mechanisms at the post-transcription level is still limited. In this study, a global screen of microRNAs (miRNAs) associated with chlorantraniliprole resistance in P. xylostella was performed. The small RNA libraries for a susceptible (CHS) and two chlorantraniliprole resistant strains (CHR, ZZ) were constructed and sequenced, and a total of 199 known and 30 novel miRNAs were identified. Among them, 23 miRNAs were differentially expressed between CHR and CHS, and 90 miRNAs were differentially expressed between ZZ and CHS, of which 11 differentially expressed miRNAs were identified in both CHR and ZZ. Using miRanda and RNAhybrid, a total of 1,411 target mRNAs from 102 differentially expressed miRNAs were predicted, including mRNAs in several groups of detoxification enzymes. The expression of several differentially expressed miRNAs and their potential targets was validated by qRT-PCR. The results may provide important clues for further study of the mechanisms of miRNA-mediated chlorantraniliprole resistance in DBM and other target insects.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Global identification of microRNAs associated with chlorantraniliprole resistance in diamondback moth Plutella xylostella (L.)

Zhu

Liu

et al. 2017

Sci Rep

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“…In some cases, entire harvests are lost in an outbreak year [13]. However, a lack of genetic information is still a barrier to our knowledge of this insect pest.…”

Section: Discussionmentioning

confidence: 99%

De novo transcriptome and expression profile analyses of the Asian corn borer (Ostrinia furnacalis) reveals relevant flubendiamide response genes

et al. 2017

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BackgroundThe Asian corn borer (ACB), Ostrinia furnacalis (Guenée), has become the most damaging insect pest of corn in Asia. However, the lack of genome or transcriptome information heavily hinders our further understanding of ACB in every aspect at a molecular level and on a genome-wide scale. Here, we used the Ion Torrent Personal Genome Machine (PGM) Sequencer to explore the ACB transcriptome and to identify relevant genes in response to flubendiamide, showing high selective activity against ACB.ResultsWe obtained 35,430 unigenes, with an average length of 716 bp, representing a dramatic expansion of existing cDNA sequences available for ACB. These sequences were annotated with Non-redundant Protein (Nr), Gene Ontology (GO), Clusters of Orthologous Groups (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) to better understand their functions. A total of 31 cytochrome P450 monooxygenases (P450s), 27 carboxyl/cholinesterases (CCEs) and 19 glutathione S-transferases (GSTs) were manually curated to construct phylogenetic trees, and 25 unigenes encoding target proteins (acetylcholinesterase, nicotinic acetylcholine receptor, gamma-aminobutyric acid receptor, glutamate-gated chloride channel, voltage-gated sodium channel and ryanodine receptor) were identified. In addition, we compared and validated the differentially expressed unigenes upon flubendiamide treatment, revealing that the genes for detoxification enzymes (P450s and esterase), calcium signaling pathways and muscle control pathways (twitchin and tropomyosin), immunoglobulin (hemolin), chemosensory protein and heat shock protein 70 were significantly overexpressed in response to flubendiamide, while the genes for cuticular protein, protease and oxidoreductase showed much lower expression levels.ConclusionThe obtained transcriptome information provides large genomic resources available for further studies of ACB. The differentially expressed gene data will elucidate the molecular mechanisms of ACB in response to the novel diamide insecticide, flubendiamide. In particular, these findings will facilitate the identification of the genes involved in insecticide resistance and the development of new compounds to control the ACB.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3431-6) contains supplementary material, which is available to authorized users.

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“…Therefore, we examined the relative transcription of several OfAgo1 miRNAs with known sequences as reported by Xu et al . (). Compared to the WT, all the examined miRNAs were significantly down‐regulated in the OfAgo1 mutants (Fig.…”

Section: Resultsmentioning

confidence: 97%

“…RNA extracted from the whole body of the 5th instar larval mutants was used for examining the expression of miRNAs using the primers provided by Xu et al . (). RNA extracted from the cuticle of the final stage larval mutants was used for examining the expression status of the pigmentation‐related genes.…”

Section: Methodsmentioning

confidence: 97%

CRISPR/Cas9‐based mutation reveals Argonaute 1 is essential for pigmentation in Ostrinia furnacalis

You

Wang

et al. 2018

Insect Science

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Ostrinia furnacalis (Lepidoptera: Pyralidae) is one of the most destructive agricultural pests in Asia. Traditional pest-management methods include sex pheromone capture, transgenic crops that produce Bacillus thuringiensis toxin, and pesticides. Although these strategies control pest populations effectively, they also cause negative side effects, including dramatically increased pesticide resistance, severe pollution, and hazards for human health. Recently developed genome editing tools provide new prospects for pest management and have been successfully used in several species. However, few examples have been reported in the agricultural pest O. furnacalis due to a lack in genomic information. In this report, we identified only one transcript of O. furnacalis Argonaute 1 (OfAgo1) gene from the genome and cloned the open reading frame. OfAgo1 presented the maximum expression at the embryo stage or in the fat body during the larval stages. To understand its function, an OfAgo1 mutant was constructed using the Clustered Regularly Interspaced Short Palindromic Repeat/RNA-guided Cas9 nuclease (CRISPR/Cas9). Mutagenesis of OfAgo1 disrupted cuticle pigmentation by down-regulating micro RNAs and pigmentation-related genes. This is the first report for the cloning and functional analysis of OfAgo1, revealing a role of OfAgo1 in cuticle pigmentation. The current report also established a CRISPR/Cas9 system in O. furnacalis, providing a new insight for pest management.

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Identification of differentially expressed microRNAs between Bacillus thuringiensis Cry1Ab-resistant and -susceptible strains of Ostrinia furnacalis

Cited by 31 publications

References 49 publications

Global identification of microRNAs associated with chlorantraniliprole resistance in diamondback moth Plutella xylostella (L.)

Global identification of microRNAs associated with chlorantraniliprole resistance in diamondback moth Plutella xylostella (L.)

De novo transcriptome and expression profile analyses of the Asian corn borer (Ostrinia furnacalis) reveals relevant flubendiamide response genes

CRISPR/Cas9‐based mutation reveals Argonaute 1 is essential for pigmentation in Ostrinia furnacalis

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