Identification of differentially expressed microRNAs in the skin of experimentally sensitized naturally affected atopic beagles by next-generation sequencing

Santoro, Domenico; Loria, Antonio Di; Mirante, Teresa; Oliveira, Duarte Mendes; Laudanna, Carmelo; Malanga, Donatella; Dattilo, Vincenzo; Iaccino, Enrico; Marsella, Rosanna; Ciaramella, Paolo

doi:10.1007/s00251-020-01162-w

Cited by 6 publications

(13 citation statements)

References 37 publications

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“…In the second study a much larger group of miRNAs was evaluated in the lesional and nonlesional skin of sensitised laboratory beagles. 35 Two miRNAs (193b and 423a) were increased in nonlesional atopic compared to healthy skin, miR-409 was increased in lesional atopic skin compared to healthy skin. MiR-193b, MiR-409 and MiR-423 were evaluated in our study as well, and not differentially expressed in allergic dogs.…”

Section: Discussionmentioning

confidence: 91%

“…This may be due to chance (the number of dogs in both studies were limited), differences in methodology or patient selection, as in our study pet dogs naturally affected by allergic dermatitis were included, while Santoro et al evaluated either healthy laboratory beagle dogs or those sensitised to house dust mites. 35 A limitation of the present study was the limited number of investigated skin specimens, especially in the group of mixed nonallergic skin diseases, as only few specimens fulfilled inclusion criteria and both mast cell tumours were excluded as a result of unsatisfactory miRNA detection. However, other studies evaluating miRNAs for cAD also had small specimen numbers.…”

Section: Discussionmentioning

confidence: 96%

“…41 Two other studies recently evaluated miRNAs in atopic dogs. 34,35 In one study, miR-39, miR-203 and miR-483 were evaluated, 34 all miRNAs outside the remit of our study. Both miR-203 and miR-483 were significantly upregulated.…”

Section: Discussionmentioning

confidence: 99%

“…Selection of miRNAs was based on a literature review, a previously performed small pilot study using a Dog miRNome miScript miRNA PCR Array (v16.0, 384 well) (Qiagen; Hilden, Germany) of one healthy and one atopic dermatitis skin sample, and on results of miRNA-expression profiles performed in beagle dogs. 35…”

Section: Mirna Selectionmentioning

confidence: 99%

“…33 To the best of the authors' knowledge to date, there are only few published studies investigating miRNAexpression in the skin of allergic dogs. 34,35 A specific miRNA expression in canine allergic dermatitis may be of great interest as a biomarker for the diagnosis and therapy of allergies in the dog.…”

Section: Introductionmentioning

confidence: 99%

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Cutaneous microRNA expression in healthy Labrador and Golden retrievers and retrievers with allergic and inflammatory skin diseases

Morlang¹,

Weber²,

Bomhard³

et al. 2021

Veterinary Dermatology

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Background -MicroRNAs (miRNA) are short, single-stranded RNA molecules that regulate gene expression in a post-transcriptional manner. Their expression is proposed to be tissue-specific and alterations in miRNA expression have been detected in many diseases.Objective -To compare miRNA expression in the skin of healthy Labrador and golden retrievers, and those with allergic and nonallergic dermatitis.Methods and materials -Formalin-fixed and paraffin-embedded (FFPE) skin specimens from seven healthy Labrador and golden retrievers, and seven dogs with allergic skin disease were collected. A further mixed nonallergic inflammation group consisted of samples from five dogs with fungal infection, demodicosis and mast cell tumours. Total RNA was extracted and miRNA primer assays for 18 target miRNAs (miR-142, miR-363, miR-18b, miR-451, miR-146a, miR-124, miR-409, miR-193b, miR-223, miR-215, miR-155, miR-423a, miR-143, miR-1839, miR-21, miR-34b, miR-146b and miR-202) were performed, with RNU6-2 and SNORD95 as miRNAs for normalisation. The selection of miRNAs for investigation was based on reported data and a pilot study evaluating miRNA extraction from FFPE tissue specimens.Results -In the two dogs with mast cell tumours, miRNA expression was undetermined for most miRNAs, so both were excluded from analysis. Although there were differences in the miRNA expression between healthy and inflamed skin, allergic and nonallergic inflammation showed similar expression patterns. Conclusion and clinical relevance -Although the number of included dogs was small, based on this study, none of the evaluated miRNAs allowed differentiation of allergic dermatitis from other inflammatory skin diseases in retriever dogs.

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Mirna Selectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cutaneous microRNA expression in healthy Labrador and Golden retrievers and retrievers with allergic and inflammatory skin diseases

Morlang¹,

Weber²,

Bomhard³

et al. 2021

Veterinary Dermatology

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Research on design of micron bubble dressing

Lin

2022

Applied Surface Science Advances

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Epithelial barrier dysfunction and associated diseases in companion animals: Differences and similarities between humans and animals and research needs

Ardicli,

Yazici

et al. 2024

Allergy

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Since the 1960s, more than 350,000 new chemicals have been introduced into the lives of humans and domestic animals. Many of them have become part of modern life and some are affecting nature as pollutants. Yet, our comprehension of their potential health risks for both humans and animals remains partial. The “epithelial barrier theory” suggests that genetic predisposition and exposure to diverse factors damaging the epithelial barriers contribute to the emergence of allergic and autoimmune conditions. Impaired epithelial barriers, microbial dysbiosis, and tissue inflammation have been observed in a high number of mucosal inflammatory, autoimmune and neuropsychiatric diseases, many of which showed increased prevalence in the last decades. Pets, especially cats and dogs, share living spaces with humans and are exposed to household cleaners, personal care products, air pollutants, and microplastics. The utilisation of cosmetic products and food additives for pets is on the rise, unfortunately, accompanied by less rigorous safety regulations than those governing human products. In this review, we explore the implications of disruptions in epithelial barriers on the well‐being of companion animals, drawing comparisons with humans, and endeavour to elucidate the spectrum of diseases that afflict them. In addition, future research areas with the interconnectedness of human, animal, and environmental well‐being are highlighted in line with the “One Health” concept.

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Identification of differentially expressed microRNAs in the skin of experimentally sensitized naturally affected atopic beagles by next-generation sequencing

Cited by 6 publications

References 37 publications

Cutaneous microRNA expression in healthy Labrador and Golden retrievers and retrievers with allergic and inflammatory skin diseases

Cutaneous microRNA expression in healthy Labrador and Golden retrievers and retrievers with allergic and inflammatory skin diseases

Research on design of micron bubble dressing

Epithelial barrier dysfunction and associated diseases in companion animals: Differences and similarities between humans and animals and research needs

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