Identification of Differentially Expressed MicroRNAs and Their Potential Target Genes in Adipose Tissue from Pigs with Highly Divergent Backfat Thickness

Xing, Kai; Zhao, Xitong; Liu, Yibing; Zhang, Fengxia; Tan, Zhen; Qi, Xiaolong; Wang, Xiangguo; Ni, Hemin; Guo, Yong; Sheng, Xihui; Wang, Chuduan

doi:10.3390/ani10040624

Cited by 12 publications

(11 citation statements)

References 46 publications

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“…Our previous report showed that miR-125 could impair the composition of porcine intramuscular fat [36]. Moreover, a study indicated that SSC-miR-141 [37], which is downregulated in high backfat tissues that target FASN, might inhibit fatty acid synthesis in pig backfat tissues. Here, we showed that miR-370-3p inhibited 3T3-L1 cell adipogenesis and changed the fatty acid composition in mature adipocytes.…”

Section: Discussionmentioning

confidence: 99%

miR-370-3p Regulates Adipogenesis through Targeting Mknk1

Zhang

et al. 2021

Molecules

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Excessive fat accumulation can lead to obesity, diabetes, hyperlipidemia, atherosclerosis, and other diseases. MicroRNAs are a class of microRNAs that regulate gene expression and are highly conserved in function among species. microRNAs have been shown to act as regulatory factors to inhibit fat accumulation in the body. We found that miR-370-3p was expressed at lower levels in the fat mass of mice on a high-fat diet than in mice on a normal control diet. Furthermore, our data showed that the overexpression of miR-370-3p significantly suppressed the mRNA expression levels of adipogenic markers. Thus, miR-370-3p overexpression reduced lipid accumulation. Conversely, the inhibition of miR-370-3p suppressed 3T3-L1 preadipocyte proliferation and promoted preadipocyte differentiation. In addition, Mknk1, a target gene of miR-370-3p, plays an opposing role in preadipocyte proliferation and differentiation. Moreover, consistent results from in vitro as well as in vivo experiments suggest that the inhibition of fat accumulation by miR-370-3p may result from the inhibition of saturated fatty acids that promote the accumulation of polyunsaturated fatty acids. In conclusion, these results suggest that miR-370-3p plays an important role in adipogenesis and fatty acid metabolism through the regulation of Mknk1.

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Section: Discussionmentioning

confidence: 99%

miR-370-3p Regulates Adipogenesis through Targeting Mknk1

Zhang

et al. 2021

Molecules

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“…SREBF-1 ( SREBP-1c ) gene: Sterol regulatory element-binding transcription factor-1c ( SREBF-1c ) was suggested to be an important lipogenic gene that has a critical role in the gene transcription mechanism and regulation of muscle fat deposition [ 62 , 102 ]. The role of SREBF-1 in fat metabolism and IMF accretion remains contradictory between studies and could be breed dependent.…”

Section: Genes Involved In Fat Metabolism and Imf Accretion In Pigsmentioning

confidence: 99%

“…Today, about 778 QTLs related to different traits have been identified and documented in the pig QTL database, pigQTLdb (see , accessed on 23 December 2021). Studies by Harper and Pethick [ 102 ] reported that the onset of marbling is located at chromosomal regions for QTL on chromosome 5 (SSC5), which is responsible for muscle growth and fat deposition. This QTL was genetically related to the RARγ gene which is involved in the transcription and expression of many other genes [ 114 ].…”

Section: Qtl Regions and Snps For Fat Metabolism And Imf Accretion In Pigsmentioning

confidence: 99%

Genes Related to Fat Metabolism in Pigs and Intramuscular Fat Content of Pork: A Focus on Nutrigenetics and Nutrigenomics

Malgwi

Halas

Grünvald

et al. 2022

Animals

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Fat metabolism and intramuscular fat (IMF) are qualitative traits in pigs whose development are influenced by several genes and metabolic pathways. Nutrigenetics and nutrigenomics offer prospects in estimating nutrients required by a pig. Application of these emerging fields in nutritional science provides an opportunity for matching nutrients based on the genetic make-up of the pig for trait improvements. Today, integration of high throughput “omics” technologies into nutritional genomic research has revealed many quantitative trait loci (QTLs) and single nucleotide polymorphisms (SNPs) for the mutation(s) of key genes directly or indirectly involved in fat metabolism and IMF deposition in pigs. Nutrient–gene interaction and the underlying molecular mechanisms involved in fatty acid synthesis and marbling in pigs is difficult to unravel. While existing knowledge on QTLs and SNPs of genes related to fat metabolism and IMF development is yet to be harmonized, the scientific explanations behind the nature of the existing correlation between the nutrients, the genes and the environment remain unclear, being inconclusive or lacking precision. This paper aimed to: (1) discuss nutrigenetics, nutrigenomics and epigenetic mechanisms controlling fat metabolism and IMF accretion in pigs; (2) highlight the potentials of these concepts in pig nutritional programming and research.

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“…The over-expression of miR-143 could accumulate more triglycerides in the adipocytes and therefore promote adipogenesis [ 28 ]. Three miRNA, miR-137, miR-144, and miR-122-5p, were defined as the candidate key regulators of fat deposition [ 31 ].…”

Section: The Mirnas Functions In Feed Efficiencymentioning

confidence: 99%

Emerging Roles of Non-Coding RNAs in the Feed Efficiency of Livestock Species

Ngoc

Davoudi

et al. 2022

Genes

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A global population of already more than seven billion people has led to an increased demand for food and water, and especially the demand for meat. Moreover, the cost of feed used in animal production has also increased dramatically, which requires animal breeders to find alternatives to reduce feed consumption. Understanding the biology underlying feed efficiency (FE) allows for a better selection of feed-efficient animals. Non-coding RNAs (ncRNAs), especially micro RNAs (miRNAs) and long non-coding RNAs (lncRNAs), play important roles in the regulation of bio-logical processes and disease development. The functions of ncRNAs in the biology of FE have emerged as they participate in the regulation of many genes and pathways related to the major FE indicators, such as residual feed intake and feed conversion ratio. This review provides the state of the art studies related to the ncRNAs associated with FE in livestock species. The contribution of ncRNAs to FE in the liver, muscle, and adipose tissues were summarized. The research gap of the function of ncRNAs in key processes for improved FE, such as the nutrition, heat stress, and gut–brain axis, was examined. Finally, the potential uses of ncRNAs for the improvement of FE were discussed.

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Identification of Differentially Expressed MicroRNAs and Their Potential Target Genes in Adipose Tissue from Pigs with Highly Divergent Backfat Thickness

Cited by 12 publications

References 46 publications

miR-370-3p Regulates Adipogenesis through Targeting Mknk1

miR-370-3p Regulates Adipogenesis through Targeting Mknk1

Genes Related to Fat Metabolism in Pigs and Intramuscular Fat Content of Pork: A Focus on Nutrigenetics and Nutrigenomics

Emerging Roles of Non-Coding RNAs in the Feed Efficiency of Livestock Species

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