Identification of disomic alien chromosome addition lines and a substitution line from monosomic alien addition lines of Chinese kale – flowering Chinese cabbage

Zhang, S. L.; Shen, Er-Qiao; Liu, J. F.; Li, Dong; Zhang, C. H.; Shen, Kuocheng; Ren, Yachao

doi:10.1080/14620316.2013.11512957

Cited by 3 publications

(2 citation statements)

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“…In contrast, disomic addition lines, which have been reported less frequently, display higher stability and fertility (Chen et al 2010a , b ; Chevre et al 1991 ; Quiros et al 1987 ; Wang et al 2006 ; Zhang et al 2013 ). To the best of our knowledge, our report is the first to describe a B. carinata-rapa disomic chromosome addition line.…”

Section: Discussionmentioning

confidence: 99%

“…The transfer rate of the BrFAE gene can be used to directly extrapolate the transmission rate of chromosome A08 as FAE is located on this chromosome (Fourmann et al 1998 ; Delourme et al 2006 ). It is well known that chromosome transmission rates are generally higher through female gametes than via male gametes (Budahn et al 2006 ; Multani et al 1994 ; Singh et al 1998 ; Zhang et al 2013 ); therefore, the focus of this study was on determining the transmission rate of chromosome A08 for male gametes by producing microspore-derived progeny and hybrid offspring by conducting crosses with a CMS B. carinata line. Both experimental approaches yielded rates of transmission of over 98%, suggesting virtually stable addition of A08 to the B. carinata genome.…”

Section: Discussionmentioning

confidence: 99%

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Development of B. carinata with super-high erucic acid content through interspecific hybridization

et al. 2021

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Key message Disomic alien chromosome addition Brassica carinata lines with super-high erucic acid content were developed through interspecific hybridization with B. juncea and characterized using molecular, cytological and biochemical techniques. Abstract Brassica carinata [A.] Braun (BBCC, 2n = 34) is a climate-resilient oilseed. Its seed oil is high in erucic acid (> 40%), rendering it well suited for the production of biofuel and other bio-based applications. To enhance the competitiveness of B. carinata with high erucic B. napus (HEAR), lines with super-high erucic acid content were developed through interspecific hybridization. To this end, a fad2B null allele from Brassica juncea (AABB, 2n = 36) was introgressed into B. carinata, resulting in a B. carinata fad2B mutant with erucic acid levels of over 50%. Subsequently, the FAE allele from B. rapa spp. yellow sarson (AA, 2n = 20) was transferred to the fad2B B. carinata line, yielding lines with erucic acid contents of up to 57.9%. Molecular analysis using the Brassica 90 K Illumina Infinium™ SNP genotyping array identified these lines as disomic alien chromosome addition lines, with two extra A08 chromosomes containing the BrFAE gene. The alien chromosomes from B. rapa were clearly distinguished by molecular cytogenetics in one of the addition lines. Analysis of microspore-derived offspring and hybrids from crosses with a CMS B. carinata line showed that the transfer rate of the A08 chromosome into male gametes was over 98%, resulting in almost completely stable transmission of an A08 chromosome copy into the progeny. The increase in erucic acid levels was accompanied by changes in the proportions of other fatty acids depending on the genetic changes that were introduced in the interspecific hybrids, providing valuable insights into erucic acid metabolism in Brassica.

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