Identification of dragon fruit (Selenicereus) species in Mekong Delta based on DNA barcode sequences

Huy, Tran Gia; Men, Tran Thanh; Thi, Nguyen; Khang, Đỗ Tấn

doi:10.13057/biodiv/d221012

Cited by 4 publications

(2 citation statements)

References 25 publications

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“…The sign "." exhibits nucleotide similar to that of the first sample; the sign "_" exhibits the missing nucleotide position; HC: Ha Chau cultivar, DD: Red cultivar, DX: Xiem cultivar, DXA: Green cultivar In comparison with previous studies in other land plants, rpoC1 gene showed highly conservative among closely related species in the genus Selenicereus (Huy et al 2021). Although rpoC1 gene exhibited 100% for amplification success, this plastid gene was not able to distinguish Dendrobium (Orchidaceae), indicated by the species resolution was only 38.89%, which was the lowest percentage among five tested markers including ITS, matK, rbcL, rpoB, and rpoC1 (Singh et al 2012).…”

Section: Rpoc1 Genementioning

confidence: 76%

Genetic diversity of Burmese grape (Baccaurea ramiflora Lour.) cultivars and Ha Chau cultivar identification based on DNA barcodes

KHANG

HUY²,

THI³

et al. 2022

Biodiversitas

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Abstract. Khang DT, Huy TG, Thi NPA, Quyen DTH, Nhu NTB, Binh BN, Men TT, Dung TN. 2022. Genetic diversity of Burmese grape (Baccaurea ramifloraLour.) cultivars and Ha Chau cultivar identification based on DNA barcodes. Biodiversitas 23: 3513-3520. Burmese grape (Baccaurea ramiflora Lour.) is an underutilized fruit tree in the Mekong Delta and has the potential for food sources as well as landscape plants,especially Ha Chau cultivar. It has been considered an indigenous plant in Can Tho city, Vietnam with specific taste. However, their genetic diversity, utilization,and identification have not been dealt with in-depth. This study aimed to characterize genetic diversity among the five cultivars of Burmese grape in the Mekong Delta and to distinguish Ha Chau from other cultivars based on DNA barcodes, namely matK, rbcL, ycf1b, rpoC1, psbK-I, atpF-H,and ITS. DNA of twelve individuals belong to five Burmese grape cultivars was extracted prior to further amplification and sequencing. Sequences were analyzed to detect variable sites and the phylogenetic tree was constructed by the Maximum Likelihood method. Based on substitution sites and indel mutations, the plastid intergenic spacer atpF-H and ycf1b gene reflected the genetic diversity in five Burmese grape cultivars. Moreover, these sequences were valuable DNA barcodes for discrimination of Ha Chau cultivar. In combination of four markers (rbcL, rpoC1, ycf1b, and psbK-I) for phylogenetic construction, our finding revealed that Ha Chau cultivar is closely related to Red cultivar with highly supported bootstrap value of 89%. Such data could be applied for reliable identification of Ha Chau cultivar from other B. ramiflora cultivars in plant authentication.

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Section: Rpoc1 Genementioning

confidence: 76%

Genetic diversity of Burmese grape (Baccaurea ramiflora Lour.) cultivars and Ha Chau cultivar identification based on DNA barcodes

KHANG

HUY²,

THI³

et al. 2022

Biodiversitas

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“…The cultivation and consumption of pitaya has increased dramatically in recent years due to its unique appearance and rich nutritional content [14]. Pitaya is reportedly grown mainly in the Americas, southern Mexico, Costa Rica, El Salvador, Venezuela, Colombia, Ecuador, Panama, Brazil, and Uruguay [15][16][17][18]. Pitaya grown in each region has different characteristics.…”

Section: About Pitayamentioning

confidence: 99%

Advances in the Understanding of Postharvest Physiological Changes and the Storage and Preservation of Pitaya

Wang,

Chen,

Luo

et al. 2024

Foods

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Highly prized for its unique taste and appearance, pitaya is a tasty, low-calorie fruit. It has a high-water content, a high metabolism, and a high susceptibility to pathogens, resulting in an irreversible process of tissue degeneration or quality degradation and eventual loss of commercial value, leading to economic loss. High quality fruits are a key guarantee for the healthy development of economic advantages. However, the understanding of postharvest conservation technology and the regulation of maturation, and senescence of pitaya are lacking. To better understand the means of postharvest storage of pitaya, extend the shelf life of pitaya fruit and prospect the postharvest storage technology, this paper analyzes and compares the postharvest quality changes of pitaya fruit, preservation technology, and senescence regulation mechanisms. This study provides research directions for the development of postharvest storage and preservation technology.

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Biodiversity of Fruit Crops and Utilization in Food and Nutritional Security

Mansyah,

Budiyanti,

Hadiati

et al. 2024

Sustainable Development and Biodiversity

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Identification of dragon fruit (Selenicereus) species in Mekong Delta based on DNA barcode sequences

Cited by 4 publications

References 25 publications

Genetic diversity of Burmese grape (Baccaurea ramiflora Lour.) cultivars and Ha Chau cultivar identification based on DNA barcodes

Genetic diversity of Burmese grape (Baccaurea ramiflora Lour.) cultivars and Ha Chau cultivar identification based on DNA barcodes

Advances in the Understanding of Postharvest Physiological Changes and the Storage and Preservation of Pitaya

Biodiversity of Fruit Crops and Utilization in Food and Nutritional Security

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