Identification of drought-induced genes from the leaves of Rangpur lime (<i>Citrus limon</i> (L) Osbeck)

Şahin‐Çevik, Mehtap; Çevik, Bayram; Topkaya-Kütük, Bengi; Yazıcı, Keziban

doi:10.1080/14620316.2017.1343101

Cited by 8 publications

(5 citation statements)

References 36 publications

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“…Strategies for drought tolerance are highly relevant in the case of rootstock selection and multiplication for ensuring continuous productivity. Rootstocks with a higher root growth ratio, better water use efficiency, higher root hydraulic conductivity, and lower osmatic conductance can withstand drought conditions while maintaining higher growth levels and mass accumulation [47,48]. After a severe drought, the recovery of tolerant rootstocks is much better than other rootstocks.…”

Section: Introductionmentioning

confidence: 99%

Effect of Three Water Regimes on the Physiological and Anatomical Structure of Stem and Leaves of Different Citrus Rootstocks with Distinct Degrees of Tolerance to Drought Stress

et al. 2021

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Citrus is grown globally throughout the subtropics and semi-arid to humid tropics. Abiotic stresses such as soil water deficit negatively affect plant growth, physiology, biochemistry, and anatomy. Herein, we investigated the effect(s) of three water regimes (control, moderate drought, and severe drought) on the physiological and anatomical structure of 10 different citrus rootstocks with different degrees of tolerance to drought stress. Brazilian sour orange and Gadha dahi performed well by avoiding desiccation and maintaining plant growth, plant water status, and biochemical characters, while Rangpur Poona nucellar (C. limonia) and Sunki × bentake were the most sensitive rootstocks at all stress conditions. At severe water stress, the highest root length (24.33 ± 0.58), shoot length (17.00 ± 1.00), root moisture content (57.67 ± 1.53), shoot moisture content (64.59 ± 1.71), and plant water potential (−1.57 ± 0.03) was observed in tolerant genotype, Brazilian sour orange. Likewise, chlorophyll a (2.70 ± 0.06), chlorophyll b (0.87 ± 0.06) and carotenoids (0.69 ± 0.08) were higher in the same genotype. The lowest H2O2 content (77.00 ± 1.00) and highest proline content (0.51 ± 0.06) were also recorded by Brazilian sour orange. The tolerance mechanism of tolerant genotypes was elucidated by modification in anatomical structures. Stem anatomy at severe drought, 27.5% increase in epidermal cell thickness, 25.4% in vascular bundle length, 30.5% in xylem thickness, 27.7% in the phloem cell area, 8% in the pith cell area, and 43.4% in cortical thickness were also observed in tolerant genotypes. Likewise, leaf anatomy showed an increase of 27.9% in epidermal cell thickness, 11.4% in vascular bundle length, 21% in xylem thickness, and 15% in phloem cell area in tolerant genotypes compared with sensitive ones. These modifications in tolerant genotypes enabled them to maintain steady nutrient transport while reducing the risk of embolisms, increasing water-flow resistance, and constant transport of nutrients across.

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Section: Introductionmentioning

confidence: 99%

Effect of Three Water Regimes on the Physiological and Anatomical Structure of Stem and Leaves of Different Citrus Rootstocks with Distinct Degrees of Tolerance to Drought Stress

et al. 2021

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“…A SSH was performed by PCR-Select cDNA Subtraction Kit (Ta-KaRa, Kusatsu, Japan) using Poly (A + ) RNA from ToCV or mock-inoculated control plants as previously outlined ( Şahin-Çevik, 2013 ; Şahin-Çevik and Moore, 2006 ; Şahin-Çevik et al, 2017 ). For construction of the subtractive cDNA library containing genes induced by ToCV, a forward subtraction was applied using the tester cDNA from ToCV-inoculated leaf samples and the driver cDNA from the leaf samples of mock-inoculated control.…”

Section: Methodsmentioning

confidence: 99%

“…Selection, sequencing and sequence analysis of clones from the subtractive library were conducted as previously described ( Şahin-Çevik, 2013 ; Şahin-Çevik and Moore, 2006 ; Şahin-Çevik et al, 2017 ) and briefly outlined here. Bacterial colonies confirmed to contain pGEM-T easy plasmids with a tomato cDNA inserts were randomly selected from the subtracted cDNA library.…”

Section: Methodsmentioning

confidence: 99%

“…To prepare nylon macroarrays, PCR amplified cDNAs of selected from the library and control genes were first denatured with 0.4 N NaOH. Then, equal amount of denatured PCR products of cDNAs, control genes and water sample were spotted in duplicates on Hybond N+ nylon membranes (Roche, Basel, Switzerland) using a Bio-Dot Microfiltration Apparatus (Bio-Rad, Hercules, CA, U.S.A.) by a vacuum application ( Şahin-Çevik, 2013 ; Şahin-Çevik et al, 2017 ). Nylon macroarrays were prepared in duplicates for hybridization with probes from ToCV-inoculated and mock-inoculated samples.…”

Section: Methodsmentioning

confidence: 99%

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Identification and Expression Analysis of Genes Induced in Response to Tomato chlorosis virus Infection in Tomato

2019

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“…It is reported that MLPs are involved in the defense against salinity in different Citrus species (Podda et al., 2014). Six MLPs were identified in Rangpur lime response to drought stress (Şahin‐Çevik et al., 2017). Previous research has demonstrated that MLPs have developed into a distinct category within the KTIs family (Selvakumar et al., 2011).…”

Section: Introductionmentioning

confidence: 99%

StMLP1, as a Kunitz trypsin inhibitor, enhances potato resistance and specifically expresses in vascular bundles during Ralstonia solanacearum infection

Wang,

et al. 2023

The Plant Journal

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SUMMARYMiraculin‐like proteins (MLPs), members of the Kunitz trypsin inhibitor (KTI) family that are present in various plants, have been discovered to have a role in defending plants against pathogens. In this study, we identified a gene StMLP1 in potato that belongs to the KTI family. We found that the expression of StMLP1 gradually increases during Ralstonia solanacearum (R. solanacearum) infection. We characterized the promoter of StMLP1 as an inducible promoter that can be triggered by R. solanacearum and as a tissue‐specific promoter with specificity for vascular bundle expression. Our findings demonstrate that StMLP1 exhibits trypsin inhibitor activity, and that its signal peptide is essential for proper localization and function. Overexpression of StMLP1 in potato can enhance the resistance to R. solanacearum. Inhibiting the expression of StMLP1 during infection accelerated the infection by R. solanacearum to a certain extent. In addition, the RNA‐seq results of the overexpression‐StMLP1 lines indicated that StMLP1 was involved in potato immunity. All these findings in our study reveal that StMLP1 functions as a positive regulator that is induced and specifically expressed in vascular bundles in response to R. solanacearum infection.

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Identification of drought-induced genes from the leaves of Rangpur lime (Citrus limon (L) Osbeck)

Cited by 8 publications

References 36 publications

Effect of Three Water Regimes on the Physiological and Anatomical Structure of Stem and Leaves of Different Citrus Rootstocks with Distinct Degrees of Tolerance to Drought Stress

Effect of Three Water Regimes on the Physiological and Anatomical Structure of Stem and Leaves of Different Citrus Rootstocks with Distinct Degrees of Tolerance to Drought Stress

Identification and Expression Analysis of Genes Induced in Response to Tomato chlorosis virus Infection in Tomato

StMLP1, as a Kunitz trypsin inhibitor, enhances potato resistance and specifically expresses in vascular bundles during Ralstonia solanacearum infection

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