Identification of drought-induced microRNAs in rice

Zhao, Bingjie; Liang, Ruqiang; Ge, Liangfa; Li, Wei; Xiao, Huasheng; Lin, Hong-Xuan; Ruan, Kangcheng; Jin, Youxin

doi:10.1016/j.bbrc.2007.01.022

Cited by 483 publications

(361 citation statements)

References 26 publications

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“…Approximately two-dozen miRNA families are highly conserved in diverse plant species indicating their essential roles in plant growth and development as well as other processes (Axtell and Bartel 2005;Sunkar and Jagadeeswaran 2008). Several conserved and species-specific miRNAs responsive to drought have been identified in Arabidopsis thaliana , Oryza sativa , Triticum dicoccoide, Medicago truncatula, Phaseolous vulagris and Populus trichocarpa (Sunkar and Zhu 2004;Liu et al 2008;Zhao et al 2007;Zhou et al 2010;Kantar et al 2011;Trindade et al 2010;Arenas-Huertero et al 2009;Lu et al 2008).…”

Section: Introductionmentioning

confidence: 99%

“…Upregulation of miRNAs in response to water deficit has been reported in several plant species (Kantar et al 2011;Trindade et al 2010;Arenas-Huertero et al 2009). miR169 was observed to respond to only Polyethanol Glycol (PEG)-mediated water deficit in rice while several miRNAs were observed to respond (both up and downregulated) to field like water deficit (Zhao et al 2007;Zhou et al 2010). miRNA expression also varies between closely related species differing in their ability to withstand stress (Kulcheski et al 2011).…”

Section: Introductionmentioning

confidence: 99%

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Analysis of biochemical variations and microRNA expression in wild (Ipomoea campanulata) and cultivated (Jacquemontia pentantha) species exposed to in vivo water stress

Ghorecha

Patel

Ingle

et al. 2013

Physiol Mol Biol Plants

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The current study analyses few important biochemical parameters and microRNA expression in two closely related species (wild but tolerant Ipomoea campanulata L. and cultivated but sensitive Jacquemontia pentantha Jacq.G.Don) exposed to water deficit conditions naturally occurring in the field. Under soil water deficit, both the species showed reduction in their leaf area and SLA as compared to well-watered condition. A greater decrease in chlorophyll was noticed in J. pentantha (~50 %) as compared to I. campanulata (20 %) under stress. By contrast, anthocyanin and MDA accumulation was greater in J. pentantha as compared to I. campanulata . Multiple isoforms of superoxide dismutases (SODs) with differing activities were observed under stress in these two plant species. CuZnSOD isoforms showed comparatively higher induction (~10-40 %) in I. campanulata than J. pentantha . MicroRNAs, miR398, miR319, miR395 miR172, and miR408 showed opposing expression under water deficit in these two plant species. Expression of miR156, miR168, miR171, miR172, miR393, miR319, miR396, miR397 and miR408 from either I. campanulata or J. pentantha or both demonstrated opposite pattern of expression to that of drought stressed Arabidopsis. The better tolerance of the wild species (I. campanulata) to water deficit could be attributed to lesser variations in chlorophyll and anthocyanin levels; and relatively higher levels of SODs than J. pentantha. miRNA expression was different in I. campanulata than J. pentantha.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analysis of biochemical variations and microRNA expression in wild (Ipomoea campanulata) and cultivated (Jacquemontia pentantha) species exposed to in vivo water stress

Ghorecha

Patel

Ingle

et al. 2013

Physiol Mol Biol Plants

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“…Changes in the expression levels of miRNAs in plant tissues subjected to biotic stresses such as viral, fungal, and bacterial infection are reported (Bazzini et al, 2007;Lu et al, 2007;Navarro et al, 2006). Recently, changes in the expression of miRNAs due to abiotic stresses such as cold, drought, salinity, oxidative stress, mechanical strain, and UV-B radiation have also been identified in plants (Lu et al, 2005;Sunkar and Zhu, 2004;Sunkar et al, 2006;Zhao et al, 2007;Zhou et al, 2007) apart from nutrient stresses, such as phosphate or sulfate starvation (Chiou et al, 2006;Fujii et al, 2005; Jones- Rhoades and Bartel, 2004), and other nutrient responses (Sunkar et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Identification of Potential microRNAs and Their Targets in Brassica rapa L.

Dhandapani

Ramchiary

Pavlidis

et al. 2011

Molecules and Cells

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MicroRNAs (miRNAs) are recently discovered, noncoding, small regulatory RNA molecules that negatively regulate gene expression. Although many miRNAs are identified and validated in many plant species, they remain largely unknown in Brassica rapa (AA 2n =, 20). B. rapa is an important Brassica crop with wide genetic and morphological diversity resulting in several subspecies that are largely grown for vegetables, oilseeds, and fodder crop production. In this study, we identified 186 miRNAs belonging to 55 families in B. rapa by using comparative genomics. The lengths of identified mature and pre-miRNAs ranged from 18 to 22 and 66 to 305 nucleotides, respectively. Comparison of 4 nucleotides revealed that uracil is the predominant base in the first position of B. rapa miRNA, suggesting that it plays an important role in miRNA-mediated gene regulation. Overall, adenine and guanine were predominant in mature miRNAs, while adenine and uracil were predominant in pre-miRNA sequences. One DNA sequence producing both sense and antisense mature miRNAs belonging to the BrMiR 399 family, which differs by 1 nucleotide at the, 20 th position, was identified. In silico analyses, using previously established methods, predicted 66 miRNA target mRNAs for 33 miRNA families. The majority of the target genes were transcription factors that regulate plant growth and development, followed by a few target genes that are involved in fatty acid metabolism, glycolysis, biotic and abiotic stresses, and other cellular processes. Northern blot and qRT-PCR analyses of RNA samples prepared from different B. rapa tissues for 17 miRNA families revealed that miRNAs are differentially expressed both quantitatively and qualitatively in different tissues of B. rapa.

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“…By now a number of stress responsive small RNAs have been identified. [9][10][11] Few of these small RNAs however have been conclusively linked to further signaling components or effector functions.…”

Section: Rna Regulation May Results In Drought Tolerance At Either Lowmentioning

confidence: 99%

Low and high ψ ways from post-transcriptional RNA regulation to drought tolerance

Deák

Jäger

Fábián

et al. 2010

Plant Signaling & Behavior

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Identification of drought-induced microRNAs in rice

Cited by 483 publications

References 26 publications

Analysis of biochemical variations and microRNA expression in wild (Ipomoea campanulata) and cultivated (Jacquemontia pentantha) species exposed to in vivo water stress

Analysis of biochemical variations and microRNA expression in wild (Ipomoea campanulata) and cultivated (Jacquemontia pentantha) species exposed to in vivo water stress

Identification of Potential microRNAs and Their Targets in Brassica rapa L.

Low and high ψ ways from post-transcriptional RNA regulation to drought tolerance

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