Identification of duplicated suppressor of cytokine signaling 3 (SOCS3) genes in blunt snout bream (Megalobrama amblycephala)

Zhao, Shanshan; Zhao, Xinyu; Wu, Chen; Zheng, Guo‐Dong; Zou, Shu‐Ming

doi:10.1016/j.cbpb.2019.110348

Cited by 13 publications

(5 citation statements)

References 35 publications

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“…It was then used for q RT-PCR analysis using specific primers (Table 6 ). Instrument, reagents, method as previously described [ 3 ]. The housekeeping gene 18S was used as the control, which had been proved to be stable between the comparison groups.…”

Section: Methodsmentioning

confidence: 99%

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The transcriptomic responses of blunt snout bream (Megalobrama amblycephala) to acute hypoxia stress alone, and in combination with bortezomib

Zhao

Pan

et al. 2022

BMC Genomics

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Background Blunt snout bream (Megalobrama amblycephala) is sensitive to hypoxia. A new blunt snout bream strain, “Pujiang No.2”, was developed to overcome this shortcoming. As a proteasome inhibitor, bortezomib (PS-341) has been shown to affect the adaptation of cells to a hypoxic environment. In the present study, bortezomib was used to explore the hypoxia adaptation mechanism of “Pujiang No.2”. We examined how acute hypoxia alone (hypoxia-treated, HN: 1.0 mg·L− 1), and in combination with bortezomib (hypoxia-bortezomib-treated, HB: Use 1 mg bortezomib for 1 kg fish), impacted the hepatic ultrastructure and transcriptome expression compared to control fish (normoxia-treated, NN). Results Hypoxia tolerance was significantly decreased in the bortezomib-treated group (LOEcrit, loss of equilibrium, 1.11 mg·L− 1 and 1.32 mg·L− 1) compared to the control group (LOEcrit, 0.73 mg·L− 1 and 0.85 mg·L− 1). The HB group had more severe liver injury than the HN group. Specifically, the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the HB group (52.16 U/gprot, 32 U/gprot) were significantly (p < 0.01) higher than those in the HN group (32.85 U/gprot, 21. 68 U/gprot). In addition, more severe liver damage such as vacuoles, nuclear atrophy, and nuclear lysis were observed in the HB group. RNA-seq was performed on livers from the HN, HB and NN groups. KEGG pathway analysis disclosed that many DEGs (differently expressed genes) were enriched in the HIF-1, FOXO, MAPK, PI3K-Akt and AMPK signaling pathway and their downstream. Conclusion We explored the adaptation mechanism of “Pujiang No.2” to hypoxia stress by using bortezomib, and combined with transcriptome analysis, accurately captured the genes related to hypoxia tolerance advantage.

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Section: Methodsmentioning

confidence: 99%

“…Blunt snout bream ( Megalobrama amblycephala ) is native to the affiliated lakes of the Yangtze River [ 1 , 2 ]. It is an herbivorous freshwater fish species with a high economic value and high disease resistance in China [ 3 , 4 ]. Blunt snout bream production output was more than 7.6 × 10 5 tons in 2019 [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

The transcriptomic responses of blunt snout bream (Megalobrama amblycephala) to acute hypoxia stress alone, and in combination with bortezomib

Zhao

Pan

et al. 2022

BMC Genomics

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“…The two sequences of CTNNB1 and CTNNB2 of H. erectus were obtained from Ensembl (http://www.ensembl.org), and SMART (https://pfam.xfam.org/) was used to confirm the conserved domain. The putative sequences of proteins, nucleotides, and alignment of amino acid sequences of these two CTNNB genes were compared and analyzed using NCBI Blast (https://blast.ncbi.nlm.nih.gov/Blast.cgi) (Zhao et al, 2020), Bio Edit 7.0.9 (Jeon et al, 2014), and ClustalX 1.83 program (Thompson et al, 1997), respectively. Phylogenetic tree analyses were constructed with MEGA 7.0.26 (Zhang et al, 2021).…”

Section: Sequence and Bioinformatics Analysesmentioning

confidence: 99%

Identification of CTNNB1 in the lined seahorse, Hippocampus erectus, and its function in innate immunity and cell proliferation

Zhang,

Wang,

et al. 2024

J World Aquaculture Soc

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Abstractβ‐Catenin (CTNNB) is a core downstream mediator in the Wnt/β‐catenin signaling pathway, which plays a role in immune regulation and cell differentiation. In the present study, two CTNNB genes were identified by genomic analysis of Hippocampus erectus and named He‐CTNNB1 and He‐CTNNB2. He‐CTNNB1 was more conserved than He‐CTNNB2. The full length of He‐CTNNB1 was 2369 bp, including an open reading frame of 2275 bp that encoded 737 amino acids containing 12 armadillo repeats. Phylogenetic analysis revealed that He‐CTNNB1 clusters had homologs in other bony fishes. Tissue distribution analysis showed the highest mRNA expression of He‐CTNNB1 in the ovary, liver, and kidney of the lined seahorse under normal physiological conditions. The transcription level of He‐CTNNB1 was significantly increased in the liver and kidney in response to LPS, poly I:C, and Edwardsiella tarda challenge (p < 0.05). In vitro overexpression of He‐CTNNB1 significantly upregulated the expression of key genes of the canonical Wnt/β‐catenin signaling pathway, such as axin2, cMyc, and cyclinD (p < 0.05), which increased cell proliferation during the wound‐healing process. Taken together, our results suggest that He‐CTNNB1 is essential for cell proliferation and the innate immune response in H. erectus.

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“…Blunt snout bream (Megalobrama amblycephala) is native species in the a liated lakes of the Yangtze River (Ke, 1965;Li, 1993). It is an herbivorous freshwater sh species with a high economic value and high disease resistance in China (Wu et al, 2020;Zhao et al, 2020). The production of blunt snout bream was more than 7.6 × 10 5 tons per year in 2019 (FBMA, 2020).…”

Section: Introductionmentioning

confidence: 99%

Functional exploration of SNP mutations in HIF2αb gene correlated with hypoxia tolerance in blunt snout bream (Megalobrama amblycephala)

Zhao

Yang

et al. 2023

Fish Physiol Biochem

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Blunt snout bream (Megalobrama amblycephala) is sensitive to hypoxia environment. Hypoxia-inducible factor (HIF) is the most critical factor in HIF pathway, which strictly regulates the hypoxia stress process of sh. In this study, we found six hifα genes in blunt snout bream that demonstrated different expression under hypoxia conditions. In HEK293T cells, all six hifαs were detected to activate HRE region by luciferase reporter assay. More importantly, we identi ed two linkage-disequilibrium SNP sites at exon 203 and 752 of hif2αb gene in blunt snout bream. Haplotype II (A 203 A 752 ) and its homozygous diplotype II (A 203 A 203 A 752 A 752 ) appeared frequently in a selected strain of blunt snout bream with hypoxia-tolerance.Diplotype II has a lower oxygen tension threshold for loss of equilibrium (LOE crit ) over a similar range of temperatures. Moreover, its erythrocyte number increased signi cantly (p < 0.05) than those in diplotype I and diplotype III strains at 48 h of hypoxia. The enzymes related with hypoxia tolerant traits, i.e. reduced glutathione, superoxide dismutase and catalase, were also signi cantly (p < 0.05) induced in diplotype II than those in diplotype I or III. In addition, the expression of epo in the liver of diplotype II was signi cantly (p < 0.01) higher than those in the diplotype I or III strains at 48 h of hypoxia. Taken together, our results found that the hypoxia-tolerant related diplotype II of hif2αb has the potential used as a molecular marker in future genetic breeding of hypoxia-tolerant strain.

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Identification of duplicated suppressor of cytokine signaling 3 (SOCS3) genes in blunt snout bream (Megalobrama amblycephala)

Cited by 13 publications

References 35 publications

The transcriptomic responses of blunt snout bream (Megalobrama amblycephala) to acute hypoxia stress alone, and in combination with bortezomib

The transcriptomic responses of blunt snout bream (Megalobrama amblycephala) to acute hypoxia stress alone, and in combination with bortezomib

Identification of CTNNB1 in the lined seahorse, Hippocampus erectus, and its function in innate immunity and cell proliferation

Functional exploration of SNP mutations in HIF2αb gene correlated with hypoxia tolerance in blunt snout bream (Megalobrama amblycephala)

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