Identification of DysI, the Immunity Factor of the Streptococcal Bacteriocin Dysgalacticin

Swe, Pearl M.; Heng, Nicholas C. K.; Cook, Gregory M.; Tagg, John; Jack, Ralph W.

doi:10.1128/aem.01707-10

Cited by 11 publications

(8 citation statements)

References 18 publications

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“…We believe that this is the first described case of co-transcription of genes involved in plasmid replication and a gene encoding a protein involved in the synthesis of EPS. However, this is not the first case in the literature of co-transcription of replication genes together with other genes, since the plasmidic dysI gene (encoding the immunity factor of the streptococcal bacteriocin dysgalacticin) is transcribed as part of the copG - repB - dysI replication-associated operon (Swe et al, 2010 ).…”

Section: Discussionmentioning

confidence: 99%

Dextransucrase Expression Is Concomitant with that of Replication and Maintenance Functions of the pMN1 Plasmid in Lactobacillus sakei MN1

et al. 2017

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The exopolysaccharide synthesized by Lactobacillus sakei MN1 is a dextran with antiviral and immunomodulatory properties of potential utility in aquaculture. In this work we have investigated the genetic basis of dextran production by this bacterium. Southern blot hybridization experiments demonstrated the plasmidic location of the dsrLS gene, which encodes the dextransucrase involved in dextran synthesis. DNA sequencing of the 11,126 kbp plasmid (pMN1) revealed that it belongs to a family which replicates by the theta mechanism, whose prototype is pUCL287. The plasmid comprises the origin of replication, repA, repB, and dsrLS genes, as well as seven open reading frames of uncharacterized function. Lb. sakei MN1 produces dextran when sucrose, but not glucose, is present in the growth medium. Therefore, plasmid copy number and stability, as well as dsrLS expression, were investigated in cultures grown in the presence of either sucrose or glucose. The results revealed that pMN1 is a stable low-copy-number plasmid in both conditions. Gene expression studies showed that dsrLS is constitutively expressed, irrespective of the carbon source present in the medium. Moreover, dsrLS is expressed from a monocistronic transcript as well as from a polycistronic repA-repB-orf1-dsrLS mRNA. To our knowledge, this is the first report of a plasmid-borne dextransucrase-encoding gene, as well as the first time that co-transcription of genes involved in plasmid maintenance and replication with a gene encoding an enzyme has been established.

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Section: Discussionmentioning

confidence: 99%

Dextransucrase Expression Is Concomitant with that of Replication and Maintenance Functions of the pMN1 Plasmid in Lactobacillus sakei MN1

et al. 2017

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“…equisimilis strain W2580, binds to membrane-bound glucose and/or the mannose phosphotransferase system (man-PTS). Once dysgalacticin has docked to the man-PTS it disrupts the cytoplasmic membrane by causing an efflux of potassium ions (K + ), which dissipates the membrane potential as seen in Streptococcus pyogenes [65]. Lactococcin A and B and some Listeria-active pediocin-like bacteriocins similarly bind man-PTS [66].…”

Section: Page 12 Of 40mentioning

confidence: 98%

Bacteriocins and their position in the next wave of conventional antibiotics

Cavera

Arthur

Kashtanov

et al. 2015

International Journal of Antimicrobial Agents

161

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“…Regarding bacteriocin produced by SDSE, Heng et al reported that dysgalacticin (DysA, UniProtKB - Q14DY1), a plasmid-encoded streptococcal bacteriocin, inhibits glucose transport [28]. DysI, the immunity factor of the streptococcal bacteriocin, suppresses the inhibitory effects of DysA [29]. However, neither dysA nor dysI were found in the three GA-SDSE strains or GG-SDSE strain ATCC 12394.…”

Section: Discussionmentioning

confidence: 99%

Comparative genome analysis of three Group A Streptococcus dysgalactiae subsp. equisimilis strains isolated in Japan

Ishihara

Ogura

Nguyen

et al. 2021

Journal of Medical Microbiology

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Introduction . Streptococcus dysgalactiae subsp. equisimilis (SDSE) is a β-hemolytic streptococcus that causes severe invasive streptococcal infections, especially in the elderly and people with underlying diseases. SDSE strains are primarily characterized by Lancefield group G or C antigens. Hypothesis/Gap Statement. We have previously reported the prevalence of Lancefield group A SDSE (GA-SDSE) strains in Japan and have analysed the draft genome sequences of these strains. As GA-SDSE is a rare type of SDSE, only one complete genome has been sequenced to date. Aim. The present study is focused on genetic characteristics of GA-SDSE strains. In order to examine molecular characteristics, we also tested growth inhibition of other streptococci by GA-SDSE. Methodology. We determined the complete genome sequences of three GA-SDSE strains by two new generation sequencing systems (short-read and long-read sequencing data). Using the sequences, we also conducted a comparative analysis of GA-SDSE and group C/G SDSE strains. In addition, we tested multiplex and quantitative PCRs targeting the GA-SDSE, group G SDSE, and S. pyogenes . Results. We found a group-specific conserved region in GA-SDSE strains that is composed of genes encoding predicted anti-bacteriocin and streptococcal lantibiotic (Sal) proteins. Multiplex and quantitative PCRs targeting the GA-SDSE-specific region were able to distinguish between GA-SDSE, other SDSE, and S. pyogenes strains. The growth of GA-SDSE was suppressed in the presence of group G SDSE, indicating a possible explanation for the low frequency of isolation of GA-SDSE. Conclusion. The comparative genome analysis shows that the genome of GA-SDSE has a distinct arrangement, enabling the differentiation between S. pyogenes , GA-SDSE, and other SDSE strains using our PCR methods.

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Identification of DysI, the Immunity Factor of the Streptococcal Bacteriocin Dysgalacticin

Cited by 11 publications

References 18 publications

Dextransucrase Expression Is Concomitant with that of Replication and Maintenance Functions of the pMN1 Plasmid in Lactobacillus sakei MN1

Dextransucrase Expression Is Concomitant with that of Replication and Maintenance Functions of the pMN1 Plasmid in Lactobacillus sakei MN1

Bacteriocins and their position in the next wave of conventional antibiotics

Comparative genome analysis of three Group A Streptococcus dysgalactiae subsp. equisimilis strains isolated in Japan

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