Identification of dysregulated pathways through <i>SLC30A8</i> protein interaction in type 1 diabetes mellitus

Abstract

&lt;abstract&gt;&lt;sec&gt; &lt;title&gt;Objective&lt;/title&gt; &lt;p&gt;The aim of the current study was to explore the gene enrichment and dysregulated pathways on the basis of interaction network analysis of &lt;italic&gt;SLC30A8&lt;/italic&gt; in type 1 diabetes mellitus (T1DM). &lt;italic&gt;SLC30A8&lt;/italic&gt; polymorphism could be characterized as a beneficial tool to identify the interacting gene in developing T1DM.&lt;/p&gt; &lt;/sec&gt;&lt;sec&gt; &lt;title&gt;Materials and methods&lt;/title&gt; &lt;p&gt;&lt;italic&gt;SLC30A8&lt;/italic&gt; interacting protein interaction network was obtained by String Interaction network Version 11.0. Ten proteins were identified interacting with &lt;italic&gt;SLC30A8&lt;/italic&gt; and were analysed by protein-protein interaction and enrichment network analysis along with Functional Enrichment analysis tool (FunRich 3.1.3) to map the gene data sets. In entire analysis, FunRich database was used as background against all annotated gene/protein list. Protein-protein interaction (PPI) and enrichment network analysis of the selected protein: &lt;italic&gt;SLC30A8&lt;/italic&gt; gene along with gene mapping and pathway enrichment were performed using FunRich 3.1.3 and String Interaction network Version 11.0.&lt;/p&gt; &lt;/sec&gt;&lt;sec&gt; &lt;title&gt;Results&lt;/title&gt; &lt;p&gt;Biological pathway grouping displayed enriched proteins in TRAIL signalling pathway (&lt;italic&gt;p&lt;/italic&gt; &amp;lt; 0.001). &lt;italic&gt;PTPRN, GAD2&lt;/italic&gt; and &lt;italic&gt;TCF7L2&lt;/italic&gt; were enriched in TRAIL Signalling pathway when &lt;italic&gt;INS&lt;/italic&gt; was made focused gene and directly interacting with &lt;italic&gt;SLC30A8&lt;/italic&gt;.&lt;/p&gt; &lt;/sec&gt;&lt;sec&gt; &lt;title&gt;Conclusions&lt;/title&gt; &lt;p&gt;TRAIL signalling pathways were enriched in T1DM. Therefore, &lt;italic&gt;SLC30A8&lt;/italic&gt; along with &lt;italic&gt;PTPRN, GAD2&lt;/italic&gt; and &lt;italic&gt;TCF7L2&lt;/italic&gt; involved in TRAIL pathway must be further explored to understand their in vivo role in T1DM.&lt;/p&gt; &lt;/sec&gt;&lt;/abstract&gt;