Identification of early and late flowering time candidate genes in endodormant and ecodormant almond flower buds

Prudencio, Ángela S.; Hoeberichts, Frank A.; Dicenta, F.; Martínez‐Gómez, Pedro; Sánchez‐Pérez, Raquel

doi:10.1093/treephys/tpaa151

Cited by 38 publications

(22 citation statements)

References 94 publications

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“…In contrast, other metabolites like p-coumaric acid and ferulic acid increased before, during, and 2 weeks after endodormancy release, indicating a potential role as biomarkers for these specialized metabolites in the transition from endodormancy to ecodormancy. In fact, the 4-COUMARATE:CoA LIGASE-LIKE 1 (4-CLL1) gene has recently been described in almond endodormancy release (Prudencio et al 2020 32 ). This tendency indicates that a large concentration of this metabolite is still necessary after endodormancy release, suggesting its metabolization into other products such as its CoA derivatives.…”

Section: Amino-acid-based Pathwaysmentioning

confidence: 99%

Ascorbic acid and prunasin, two candidate biomarkers for endodormancy release in almond flower buds identified by a nontargeted metabolomic study

et al. 2020

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Temperate fruit trees belonging to Prunus species have the ability to suspend (induce dormancy) and resume growth periodically in response to environmental and seasonal conditions. Endodormancy release requires the long-term accumulation of chill. Upon accumulation of cultivar-specific chill requirements, plants enter the state of ecodormancy, which means the ability to grow has been restored, depending on the fulfilment of heat requirements. As many different metabolic pathways are implicated in endodormancy release, we have performed a metabolomic analysis, using the ultra-high-performance liquid chromatography–quadrupole time-of-flying (UPLC–QToF) technique. We assayed flower buds in different stages of endodormancy in four almond cultivars with different flowering times: the extra-early Desmayo Largueta, the late Antoñeta, the extra-late Penta, and the ultra-late Tardona. An orthogonal projection to latent-structure discriminant-analysis model was created to observe differences between endodormant and ecodormant flower buds. The metabolites showing the most significant variation were searched against the Metlin, HMDB, and KEGG libraries, which allowed us to identify 87 metabolites. These metabolites were subsequently assigned to specific pathways, such as abscisic acid biosynthesis, phenylpropanoid biosynthesis, and D-sorbitol metabolism, among others. The two metabolites that exhibited the most significant variations in all the cultivars studied with fold changes of up to 6.49 were ascorbic acid and prunasin. For the first time, these two metabolites have been proposed as potential biomarkers for endodormancy release in almond. Given the high synteny present between the Rosaceae species, these results could be extrapolated to other important crops like peach, plum, cherry, or apricot, among others.

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Section: Amino-acid-based Pathwaysmentioning

confidence: 99%

Ascorbic acid and prunasin, two candidate biomarkers for endodormancy release in almond flower buds identified by a nontargeted metabolomic study

et al. 2020

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“…Similar transcriptome profiles were observed in other Prunus species ( Habu and Tao, 2014 ; Gómez et al, 2019 ; Iqbal et al, 2021 ). Compared with the molecular mechanism on flower opening process in this study ( Figure 3 ), similar processes have been activated and modulated even in dormancy period, with genes for sugar synthesis and mobilization, lipid peroxidation, coumarate metabolism, transmembrane transport, cell wall remodeling, and ABA synthesis and signaling ( Prudencio et al, 2021 ) as well as gametophyte development in Prunus species ( Julian et al, 2011 ; Rios et al, 2013 ). One exceptional difference was that genes for “cell wall organization or biogenesis” (GO:0071554) were activated in the flower opening process, which would be required for the rapid cell expansion in the exponential enlargement of floral organs and petal emergence movement like fruit enlargement ( Zanchin et al, 1994 ).…”

Section: Discussionmentioning

confidence: 57%

Cherry Blossom Forecast Based on Transcriptome of Floral Organs Approaching Blooming in the Flowering Cherry (Cerasus × yedoensis) Cultivar ‘Somei-Yoshino’

et al. 2022

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To gain insights into the genetic mechanisms underlying blooming and petal movement in flowering cherry (Cerasus × yedoensis), we performed time-course RNA-seq analysis of the floral buds and open-flowers of the most popular flowering cherry cultivar, ‘Somei-Yoshino.’ Independent biological duplicate samples of floral buds and open-flowers were collected from ‘Somei-Yoshino’ trees grown at three different locations in Japan. RNA-seq reads obtained from floral bud and open-flower samples collected in the current study (in 2019) and in a previous study (in 2017) were aligned against the genome sequence of ‘Somei-Yoshino’ to quantify gene transcript levels. Clustering analysis of RNA-seq reads revealed dynamic changes in the transcriptome, with genes in seven modules predominantly expressed at specific time points, ranging from 5 weeks before flowering to 2 weeks after flowering. Based on the identified gene modules and Gene Ontology (GO) terms enriched at different floral stages, we speculate that the genetic mechanisms underlying petal movement and flower opening in cherry involve the processes of development, cell wall organization, reproduction, and metabolism, which are executed by genes encoding transcription factors, phytohormones, transporters, and polysaccharide metabolic enzymes. Furthermore, we established a statistical model for cherry bloom forecasting, based on gene expression levels as RNA markers at different time points before flowering.

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“…However, complete frost damage (without signs of regeneration) was not recorded, and the rose plants pruned to the ground grew intensively until autumn (Figure 5). Woody plants of the temperate zone can survive the winter thanks to bud dormancy and cold hardiness [20,58], a process which could be disrupted by climate changes, as in the case of Vitis [20,59]. Winter hardiness can be connected with the prolongation of the growing season [50] as well as hardening and dehardening [20], because the decrease of low minimum temperature was only correlated with higher frost damage in December and March (Table 3).…”

Section: Discussionmentioning

confidence: 99%

“…Webb]. The informations of this research can be used by the breeding programs of new cultivars by a climate change in temperate regions [59].…”

Section: Discussionmentioning

confidence: 99%

Trends in the Phenology of Climber Roses under Changing Climate Conditions in Mazovia Lowland in Central Europe

Monder¹

2021

Preprint

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The genetic pool of valuable old ornamental cultivars and their in situ maintenance may be threated by climate change. Meanwhile, the ornamental plants like roses make up an important share of both gardens and urban green spaces, where they are particularly vulnerable to multistress growth conditions. The aim of this research was to evaluate the effect of changing climatic conditions on growth and flowering of 11 historic climber roses through long-term studies (2000-2017) conducted in Central Europe. The evaluation of plants consisted of assessment of frost damage and the timing of early phenological stages (starting of bud break, leaf unfolding) as well as gathering data on beginning, fullness and end of flowering and its abundance. Frost damage was not recorded in any year only in ‘Mme Plantier’, and did not occur for any cultivar after the winter in the years 2007, 2008, and 2014. Only a little damage to one-year shoots was recorded after the winter in the years 2015-2017. Frost damage to ‘Alberic Barbier’, ‘Albertine’, ‘Chaplin's Pink Climber’, ‘Orange Triumph clg’ and ‘Venusta Pendula’ led to pruning to ground level in every year excluding those listed above. Frost damage of once blooming roses limited their flowering; however, the many-year data-sets showed a trend for decreased frost damage and improved abundance of flowering, and these results can be interpreted as a response to the increase of average air temperature. The timing of bud breaking and leaf development in all climber roses was strictly correlated with average air temperature in the dormancy period. The reactions of climber roses to weather conditions confirmed the influence of climatic changes on ornamental crop plants in Central Europe, introducing the potential possibility for the wider application of climber roses, but without certainty of flowering every year.

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Identification of early and late flowering time candidate genes in endodormant and ecodormant almond flower buds

Cited by 38 publications

References 94 publications

Ascorbic acid and prunasin, two candidate biomarkers for endodormancy release in almond flower buds identified by a nontargeted metabolomic study

Ascorbic acid and prunasin, two candidate biomarkers for endodormancy release in almond flower buds identified by a nontargeted metabolomic study

Cherry Blossom Forecast Based on Transcriptome of Floral Organs Approaching Blooming in the Flowering Cherry (Cerasus × yedoensis) Cultivar ‘Somei-Yoshino’

Trends in the Phenology of Climber Roses under Changing Climate Conditions in Mazovia Lowland in Central Europe

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