Identification of Ehrlichia Species in Blood Smear

Tami, Irene; Martínez, J.I. Hermoso; Tami, Manuel; Redondo, María Cristina; Finol, Héctor J.; Simonovis, N.

doi:10.1097/00019048-199612000-00011

Cited by 6 publications

(11 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Thus, in Lara, Venezuela, these infections may not be prevalent. Despite the reports of rickettsiae-like organisms in human platelets in Venezuela (1,25), there has been no evidence for human infection with A. platys in the present study. Further studies are needed to clarify potential human infection with A. platys in Venezuela.…”

Section: Discussioncontrasting

confidence: 85%

“…In Venezuela, while there was a report on microscopic observations of blood smear (2), prevalence of A. platys infection of dogs has not been determined. Furthermore, microscopic observations of A. platys-like organisms in human platelets were reported in Venezuela (1,25), and A. platys infection has been confirmed by PCR in one single dog only (24). The present study, therefore, examined the prevalence of A. platys infection in populations at high risk of infection: military dogs, ticks removed from these dogs, and trainers of these dogs in Venezuela, and determined the molecular characteristics of the isolated strains.…”

Section: Introductionmentioning

confidence: 92%

See 1 more Smart Citation

Prevalence and molecular analysis of Anaplasma platys in dogs in Lara, Venezuela

Huang

Ünver

Perez

et al. 2005

Braz. J. Microbiol.

View full text Add to dashboard Cite

Blood specimens from clinically normal military dogs and their trainers, in Lara, Venezuela were screened for Anaplasma platys, A. phagocytophilum, or Ehrlichia ewingii using 16S rRNA PCR tests. Sixteen percent (7/ 43) of dog specimens were positive by A. platys PCR test followed by sequencing of the PCR products, and all human blood specimens [25] were negative. All specimens from these dogs and humans were PCR negative for E. ewingii or A. phagocytophilum. Twelve Rhipicephalus sanguineus ticks removed from these dogs were negative for A. platys by reverse transcription PCR test. Almost the entire 16S rRNA gene (1,364 bp) and groESL operon (1,646 bp) sequences of A. platys isolated from a dog were determined, revealing that both sequences were closely related to the sequences of an A. platys strain detected in R. sanguineus ticks from the Democratic Republic of Congo.

show abstract

Section: Discussioncontrasting

confidence: 85%

Section: Introductionmentioning

confidence: 92%

Prevalence and molecular analysis of Anaplasma platys in dogs in Lara, Venezuela

Huang

Ünver

Perez

et al. 2005

Braz. J. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Integrated metric of evidence values could not be calculated for the remaining 16 studies. 3,21,61,74,95,97,[139][140][141][142][143][144][145][146][147][148] Of the 11 studies with an IME value, 10 specifically studied E. canis. 70,85,93,100,103,104,114,116,117,123 One study did not indicate the Ehrlichia species involved, 107 but E. canis was presumed based on geographic locale.…”

Section: Evidence Summarymentioning

confidence: 99%

“…90 Seven additional studies for which IME values could not be calculated documented thrombocytopenia in dogs with anaplasmosis. 21,46,117,144,148,156,157 In a study of 18 dogs PCR-positive for A. phagocytophilum, 16 were thrombocytopenic. 156 Six of the 9 dogs tested were positive for PSAIG, suggesting an immune-mediated pathogenesis.…”

Section: Evidence Summarymentioning

confidence: 99%

ACVIM consensus statement on the diagnosis of immune thrombocytopenia in dogs and cats

LeVine,

Kidd,

Garden

et al. 2024

Veterinary Internal Medicne

View full text Add to dashboard Cite

Immune thrombocytopenia (ITP) is the most common acquired primary hemostatic disorder in dogs. Immune thrombocytopenia less commonly affects cats but is an important cause of mortality and treatment‐associated morbidity in both species. Immune thrombocytopenia remains a diagnosis of exclusion for which diagnostic guidelines are lacking. Primary, or non‐associative, ITP refers to autoimmune platelet destruction. Secondary, or associative, ITP arises in response to an underlying disease trigger. However, evidence for which comorbidities serve as ITP triggers has not been systematically evaluated. To identify key diagnostic steps for ITP and important comorbidities associated with secondary ITP, we developed 12 Population Evaluation/Exposure Comparison Outcome (PECO) format questions. These questions were addressed by evidence evaluators utilizing a literature pool of 287 articles identified by the panelists using a structured search strategy. Evidence evaluators, using panel‐designed templates and data extraction tools, summarized evidence and created guideline recommendations that then were integrated by diagnosis and comorbidity domain chairs. The revised PECO responses underwent a Delphi survey process to reach consensus on final guidelines. A combination of panel expertise and PECO responses were employed to develop algorithms for diagnosis of ITP in dogs and cats, which also underwent 4 iterations of Delphi review. Comorbidity evidence evaluators employed an integrated measure of evidence (IME) tool to determine evidence quality for each comorbidity; IME values combined with evidence summaries for each comorbidity were integrated to develop ITP screening recommendations, which also were subjected to Delphi review. Commentary was solicited from multiple relevant professional organizations before finalizing the consensus. The final consensus statement provides clinical guidelines for the diagnosis of, and underlying disease screening for, ITP in dogs and cats. The systematic consensus process identified numerous knowledge gaps that should guide future studies. This statement is a companion manuscript to the ACVIM Consensus Statement on the Treatment of Immune Thrombocytopenia.

show abstract

“…(12). Más tarde, se publicaron los resultados de otra investigación en la que se estudió una población más amplia de animales y seres humanos y se realizó microscopia electrónica de la sangre de un canino con ehrliquiosis en fase aguda de la enfermedad(13). En una tercera publicación se informó de la detección de mórulas en plaquetas mediante el examen de frotis sanguíneos de dos pacientes con un cuadro clínico compatible con la enfermedad.…”

unclassified

Identificación morfológica de Ehrlichia sp. en las plaquetas de pacientes con infección por virus de la inmunodeficiencia humana, en Venezuela

Tami¹,

Tamí-Maury²

2004

Rev Panam Salud Publica

View full text Add to dashboard Cite

show abstract

Identification of Ehrlichia Species in Blood Smear

Cited by 6 publications

References 0 publications

Prevalence and molecular analysis of Anaplasma platys in dogs in Lara, Venezuela

Prevalence and molecular analysis of Anaplasma platys in dogs in Lara, Venezuela

ACVIM consensus statement on the diagnosis of immune thrombocytopenia in dogs and cats

Identificación morfológica de Ehrlichia sp. en las plaquetas de pacientes con infección por virus de la inmunodeficiencia humana, en Venezuela

Contact Info

Product

Resources

About