Identification of Eimeria acervulina conoid antigen using chicken monoclonal antibody

Matsubayashi, Makoto; Minoura, Chisa; Kimura, Shige; Tani, Hiroyuki; Furuya, Masaru; Lillehoj, Hyun S.; Matsuda, Haruo; Takenaka, Shigeo; Hatta, Takeshi; Tsuji, Naotoshi; Sasai, Kazumi

doi:10.1007/s00436-016-5185-0

Cited by 4 publications

(2 citation statements)

References 27 publications

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“…Also, antibody may not recognise parasite extracts ab initio [ 72 ] (Fig. 2 ) because of protein self-activation/re-naturation [ 118 , 143 ], isoforms and clusters [ 42 , 134 ]. Various Eimeria stages may show simultaneous or differential expression of some proteins [ 112 , 144 ], which invariably depend on level of expression, importance to parasite stage, host response [ 91 ], limitation (or liberality) of fluorescent antibody [ 66 ] and gene splicing [ 52 ].…”

Section: Hindsightmentioning

confidence: 99%

“…Eimeria sporozoites, merozoites and trophozoites (zoite stages) possess sub-cellular structures [36] such as apicoplasts, rhoptries, micronemes, conoids, dense granules, polar rings and sub-pellicular microtubules [37] as well as Golgi apparatus, cytoskeleton-associated structures [38][39][40], inner membrane complexes and acidocalcisomes [32,41] and, specifically, refractile bodies (RBs) and amylopectin granules [32,42]. Apical, membrane-bound and heat shock proteins and proteases have been well studied [43].…”

Section: Introductionmentioning

confidence: 99%

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Eimeria proteins: order amidst disorder

Olajide

Yang

et al. 2022

Parasites Vectors

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Apicomplexans are important pathogens that cause severe infections in humans and animals. The biology and pathogeneses of these parasites have shown that proteins are intrinsically modulated during developmental transitions, physiological processes and disease progression. Also, proteins are integral components of parasite structural elements and organelles. Among apicomplexan parasites, Eimeria species are an important disease aetiology for economically important animals wherein identification and characterisation of proteins have been long-winded. Nonetheless, this review seeks to give a comprehensive overview of constitutively expressed Eimeria proteins. These molecules are discussed across developmental stages, organelles and sub-cellular components vis-à-vis their biological functions. In addition, hindsight and suggestions are offered with intention to summarise the existing trend of eimerian protein characterisation and to provide a baseline for future studies. Graphical Abstract

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Section: Hindsightmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Eimeria proteins: order amidst disorder

Olajide

Yang

et al. 2022

Parasites Vectors

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Vaccination with Eimeria tenella elongation factor-1α recombinant protein induces protective immunity against E. tenella and E. maxima infections

Lin

Lillehoj

Lee

et al. 2017

Veterinary Parasitology

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Protection against Eimeria intestinalis infection in rabbits immunized with the recombinant elongation factors EF1α and EFG

He,

Hao,

Xiong

et al. 2023

Infect Immun

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Eimeria intestinalis is the most pathogenic species of rabbit coccidiosis, causing weight loss, diarrhea, and even acute death. The currently used anticoccidial drugs against E. intestinalis in rabbits are associated with drug resistance and residues. Immunological control might be a potential alternative. We cloned and expressed the E. intestinalis recombinant EF1α and EFG (r Ei -EF1α and r Ei -EFG, respectively). Rabbits were immunized subcutaneously every 14 days with 100 µg of r Ei -EF1α and r Ei -EFG and followed by 5 × 10 4 E. intestinalis sporulated oocysts orally challenge. Serum samples were collected every 7 days to measure the levels of specific antibodies and cytokines. On post-challenge day 14, rabbits were sacrificed and the anticoccidial index was evaluated. The rabbits of PBS challenged groups exhibited anorexia, diarrhea, marked intestinal wall thickening, and white nodules that formed patches, while rabbits from the r Ei -EF1α or r Ei -EFG challenged group exhibited milder symptoms. The r Ei -EF1α group showed a 75.18% oocyst reduction and 89.01%wt gain; the r Ei -EFG group had a 60.58% oocyst reduction and 56.04%wt gain. After vaccination, specific IgG levels increased and stayed high ( P < 0.05). The IL-4 and IL-2 levels of r Ei -EF1α immunized groups showed a significant increase after immunization ( P < 0.05). Both r Ei -EF1α and r Ei -EFG could induce humoral and cellular immune responses. In contrast, rabbits immunized with r Ei -EF1α were better protected from challenge by E. intestinalis than r Ei -EFG.

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Identification of Eimeria acervulina conoid antigen using chicken monoclonal antibody

Cited by 4 publications

References 27 publications

Eimeria proteins: order amidst disorder

Eimeria proteins: order amidst disorder

Vaccination with Eimeria tenella elongation factor-1α recombinant protein induces protective immunity against E. tenella and E. maxima infections

Protection against Eimeria intestinalis infection in rabbits immunized with the recombinant elongation factors EF1α and EFG

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