Identification of Endosymbionts in Ticks by Broad-Range Polymerase Chain Reaction and Electrospray Ionization Mass Spectrometry

Rounds, Megan A.; Crowder, Christopher D; Matthews, Heather; Philipson, Curtis A.; Scoles, Glen A.; Ecker, David J.; Schutzer, Steven E.; Eshoo, Mark W.

doi:10.1603/me12038

Cited by 37 publications

(35 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several Rickettsia spp. are known to exist as endosymbionts in ixodid ticks (Rounds et al 2012). Endosymbionts were shown not to express certain proteins associated with pathogenicity, like the ompA gene.…”

Section: Discussionmentioning

confidence: 99%

Molecular Detection ofRickettsia africae, Rickettsia aeschlimannii,andRickettsia sibirica mongolitimonaein Camels andHyalommaspp. Ticks from Israel

Kleinerman

Baneth

Mumcuoglu³

et al. 2013

Vector-Borne and Zoonotic Diseases

View full text Add to dashboard Cite

In this study, we aimed to identify and genetically characterize spotted fever group (SFG) rickettsiae in ticks, domestic one-humped camels, and horses from farms and Bedouin communities in southern Israel. A total of 618 ixodid ticks (Hyalomma dromedarii, Hyalomma turanicum, Hyalomma excavatum, and Hyalomma impeltatum) collected from camels and horses, as well as 152 blood samples from 148 camels and four horses were included in the study. Initial screening for rickettsiae was carried out by targeting the gltA gene. Positive samples were further analyzed for rickettsial ompA, 17kDa, ompB, and 16S rRNA genes. Rickettsia aeschlimannii DNA was detected in the blood of three camels and 14 ticks (H. dromedarii, H. turanicum, and H. excavatum). Rickettsia africae was found in six ticks (H. turanicum, H. impeltatum, H. dromedarii, and H. excavatum). In addition, Rickettsia sibirica mongolitimonae was detected in one H. turanicum tick. These findings represent the first autochthonous detection of R. africae in Israel. Previous detections of R. africae in Asia were reported from the Sinai Peninsula (Egypt) and Istanbul, only. Furthermore, we report for the first time the finding of R. aeschlimannii in H. turanicum and H. excavatum ticks, as well as the first identification of R. sibirica mongolitimonae in H. turanicum ticks. The tick species identified to harbor R. africae and other SFG rickettsiae have been reported to occasionally feed on people, and, therefore, physicians should be aware of the possible exposure of local communities and travelers, especially those in contact with camels, to these tick-borne rickettsial pathogens.

show abstract

Section: Discussionmentioning

confidence: 99%

Molecular Detection ofRickettsia africae, Rickettsia aeschlimannii,andRickettsia sibirica mongolitimonaein Camels andHyalommaspp. Ticks from Israel

Kleinerman

Baneth

Mumcuoglu³

et al. 2013

Vector-Borne and Zoonotic Diseases

View full text Add to dashboard Cite

show abstract

“…Many species of ticks are associated with distinct endosymbiontic bacteria (Rounds et al, 2012). In this study, we detected multiple symbiontic SFG Rickettsia.…”

Section: Discussionmentioning

confidence: 99%

Detection of human bacterial pathogens in ticks collected from Louisiana black bears (Ursus americanus luteolus)

Leydet

Liang

2013

Ticks and Tick-borne Diseases

View full text Add to dashboard Cite

There are 4 major human-biting tick species in the northeastern United States, which include: Amblyomma americanum, Amblyomma maculatum, Dermacentor variabilis, and Ixodes scapularis. The black bear is a large mammal that has been shown to be parasitized by all the aforementioned ticks. We investigated the bacterial infections in ticks collected from Louisiana black bears (Ursus americanus subspecies luteolus). Eighty-six ticks were collected from 17 black bears in Louisiana from June 2010 to March 2011. All 4 common human-biting tick species were represented. Each tick was subjected to polymerase chain reaction (PCR) targeting select bacterial pathogens and symbionts. Bacterial DNA was detected in 62% of ticks (n=53). Rickettsia parkeri, the causative agent of an emerging spotted fever group rickettsiosis, was identified in 66% of A. maculatum, 28% of D. variabilis, and 11% of I. scapularis. The Lyme disease bacterium, Borrelia burgdorferi, was detected in 2 I. scapularis, while one Am. americanum was positive for Borrelia bissettii, a putative human pathogen. The rickettsial endosymbionts Candidatus Rickettsia andeanae, rickettsial endosymbiont of I. scapularis, and Rickettsia amblyommii were detected in their common tick hosts at 21%, 39%, and 60%, respectively. All ticks were PCR-negative for Anaplasma phagocytophilum, Ehrlichia spp., and Babesia microti. This is the first reported detection of R. parkeri in vector ticks in Louisiana; we also report the novel association of R. parkeri with I. scapularis. Detection of both R. parkeri and Bo. burgdorferi in their respective vectors in Louisiana demands further investigation to determine potential for human exposure to these pathogens.

show abstract

“…In some areas, it is detected in 90% to 100% of I. scapularis ticks (104,145,146), suggesting that this species is an endosymbiont. Other incompletely described SFG rickettsiae that have been detected in I. scapularis include Rickettsia sp.…”

Section: North and Central Americamentioning

confidence: 99%

Update on Tick-Borne Rickettsioses around the World: a Geographic Approach

et al. 2013

View full text Add to dashboard Cite

SUMMARY Tick-borne rickettsioses are caused by obligate intracellular bacteria belonging to the spotted fever group of the genus Rickettsia . These zoonoses are among the oldest known vector-borne diseases. However, in the past 25 years, the scope and importance of the recognized tick-associated rickettsial pathogens have increased dramatically, making this complex of diseases an ideal paradigm for the understanding of emerging and reemerging infections. Several species of tick-borne rickettsiae that were considered nonpathogenic for decades are now associated with human infections, and novel Rickettsia species of undetermined pathogenicity continue to be detected in or isolated from ticks around the world. This remarkable expansion of information has been driven largely by the use of molecular techniques that have facilitated the identification of novel and previously recognized rickettsiae in ticks. New approaches, such as swabbing of eschars to obtain material to be tested by PCR, have emerged in recent years and have played a role in describing emerging tick-borne rickettsioses. Here, we present the current knowledge on tick-borne rickettsiae and rickettsioses using a geographic approach toward the epidemiology of these diseases.

show abstract

Identification of Endosymbionts in Ticks by Broad-Range Polymerase Chain Reaction and Electrospray Ionization Mass Spectrometry

Cited by 37 publications

References 41 publications

Molecular Detection ofRickettsia africae, Rickettsia aeschlimannii,andRickettsia sibirica mongolitimonaein Camels andHyalommaspp. Ticks from Israel

Molecular Detection ofRickettsia africae, Rickettsia aeschlimannii,andRickettsia sibirica mongolitimonaein Camels andHyalommaspp. Ticks from Israel

Detection of human bacterial pathogens in ticks collected from Louisiana black bears (Ursus americanus luteolus)

Update on Tick-Borne Rickettsioses around the World: a Geographic Approach

Contact Info

Product

Resources

About