Identification of Enterobacteriaceae isolates with OXA-48 and coproduction of OXA-181 and NDM-1 in Norway

Samuelsen, Ørjan; Naseer, Umaer; Karah, Nabil; Lindemann, Paul Christoffer; Kanestrøm, Anita; Leegaard, Truls Michael; Sundsfjord, Arnfinn

doi:10.1093/jac/dkt058

Cited by 34 publications

(33 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Those associated resistance determinants are AmpC cephalosporinases, clavulanic acid inhibited expanded-spectrum β -lactamases (ESBLs), other types of carbapenemases (OXA-48-, VIM-, and KPC-types), and resistance to aminoglycosides (16S RNA methylases), to quinolones (Qnr), to macrolides (esterases), to rifampicin (rifampicin-modifying enzymes), to chloramphenicol, and to sulfamethoxazole [4–9]. Consequently, most of the NDM-1 producers remain susceptible only to two bactericidal antibiotics (colistin and fosfomycin) and a single bacteriostatic antibiotic (tigecycline) [10, 11] (Figure 1).…”

Section: Clinical Impact Of the Antibiotic Resistance Patterns Of mentioning

confidence: 99%

Worldwide Dissemination of the NDM-Type Carbapenemases in Gram-Negative Bacteria

Dortet

Poirel

Nordmann

2014

BioMed Research International

420

313

View full text Add to dashboard Cite

The emergence of one of the most recently described carbapenemases, namely, the New Delhi metallo-lactamase (NDM-1), constitutes a critical and growingly important medical issue. This resistance trait compromises the efficacy of almost all lactams (except aztreonam), including the last resort carbapenems. Therapeutical options may remain limited mostly to colistin, tigecycline, and fosfomycin. The main known reservoir of NDM producers is the Indian subcontinent whereas a secondary reservoir seems to have established the Balkans regions and the Middle East. Although the spread of bla NDM-like genes (several variants) is derived mostly by conjugative plasmids in Enterobacteriaceae, this carbapenemase has also been identified in P. aeruginosa and Acinetobacter spp. Acinetobacter sp. may play a pivotal role for spreading bla NDM genes for its natural reservoir to Enterobacteriaceae. Rapid diagnostic techniques (Carba NP test) and screening of carriers are the cornerstone to try to contain this outbreak which threatens the efficacy of the modern medicine.

show abstract

Section: Clinical Impact Of the Antibiotic Resistance Patterns Of mentioning

confidence: 99%

Worldwide Dissemination of the NDM-Type Carbapenemases in Gram-Negative Bacteria

Dortet

Poirel

Nordmann

2014

BioMed Research International

420

313

View full text Add to dashboard Cite

show abstract

“…Since the identification of OXA-181 in India in 2007, OXA-181-producing Enterobacteriaceae has been reported from several other countries in the Indian subcontinent, i.e., Bangladesh (9) and Sri Lanka (10) and potentially Nepal, as a Nepalese patient hospitalized in New Zealand was found to be carrying an OXA-181-producing K. pneumoniae (11). Outside the subcontinent, Enterobacteriaceae isolates producing OXA-181 have been found in Canada (12), France (13), the Netherlands (14), New Zealand (in a patient from Nepal) (11), Norway (in a patient from Romania) (15), Oman (16), Romania (17), Singapore (9), South Africa (18), and United Kingdom (in a patient from India) (19). In most cases (9, 11-14, 19, 20), the patients from whom OXA-181-producing Enterobacteriaceae were recovered had a recent travel history to the Indian subcontinent.…”

mentioning

confidence: 99%

“…The only exception was in a Citrobacter freundii strain; bla NDM-1 was not detected on the plasmid carrying bla OXA-181 , suggesting that bla NDM-1 is likely located on a different plasmid. It would be interesting to explore whether bla NDM-1 was located on an IncX3 plasmid within those strains carrying bla NDM-1 and bla OXA-181 (9,10,12,(15)(16)(17)29). On the other hand, as WCHEC14828 has no bla NDM-1 , it may also be possible that an IncX3 plasmid carrying bla OXA-181 has replaced the IncX3 plasmid carrying bla NDM-1 due to plasmid incompatibility.…”

mentioning

confidence: 99%

First Report of OXA-181-Producing Escherichia coli in China and Characterization of the Isolate Using Whole-Genome Sequencing

Liu

Feng

et al. 2015

Antimicrob Agents Chemother

View full text Add to dashboard Cite

eWe report the first OXA-181-producing strain in China. bla OXA-181 was found in sequence type 410 (ST410) Escherichia coli strain WCHEC14828 from a Chinese patient without recent travel history. Genome sequencing and conjugation experiments were performed. bla OXA-181 was carried on a 51-kb self-transmissible IncX3 plasmid and was linked with qnrS1, a quinolone resistance gene. bla OXA-181 was introduced onto the IncX3 plasmid from a ColE2-type plasmid, and IncX3 plasmids have the potential to mediate the dissemination of bla OXA-181 .

show abstract

“…On the Indian subcontinent, where NDM-1 is endemic, variants of OXA-48, including OXA-181 and OXA-232, also seem to be spreading among Enterobacteriaceae (6,7). As a consequence of the rapid spread of these two groups of carbapenemases, the coproduction of NDM-1 and OXA-48 group carbapenemases is also increasingly reported in Enterobacteriaceae (8)(9)(10)(11)(12)(13)(14)(15)(16). However, the genetics supporting the coacquisition of multiple carbapenemase genes have not been elucidated.…”

mentioning

confidence: 99%

Whole-Genome Assembly of Klebsiella pneumoniae Coproducing NDM-1 and OXA-232 Carbapenemases Using Single-Molecule, Real-Time Sequencing

Doi

Hazen

Boitano

et al. 2014

Antimicrob Agents Chemother

View full text Add to dashboard Cite

The whole-genome sequence of a carbapenem-resistant Klebsiella pneumoniae strain, PittNDM01, which coproduces NDM-1 and OXA-232 carbapenemases, was determined in this study. The use of single-molecule, real-time (SMRT) sequencing provided a closed genome in a single sequencing run. K. pneumoniae PittNDM01 has a single chromosome of 5,348,284 bp and four plasmids: pPKPN1 (283,371 bp), pPKPN2 (103,694 bp), pPKPN3 (70,814 bp), and pPKPN4 (6,141 bp). The contents of the chromosome were similar to that of the K. pneumoniae reference genome strain MGH 78578, with the exception of a large inversion spanning 23.3% of the chromosome. In contrast, three of the four plasmids are unique. The plasmid pPKPN1, an IncHI1B-like plasmid, carries the bla NDM-1 , armA, and qnrB1 genes, along with tellurium and mercury resistance operons. bla NDM-1 is carried on a unique structure in which Tn125 is further bracketed by IS26 downstream of a class 1 integron. The IncFIA-like plasmid pPKPN3 also carries an array of resistance elements, including bla CTX-M-15 and a mercury resistance operon. The ColE-type plasmid pPKPN4 carrying bla OXA-232 is identical to a plasmid previously reported from France. SMRT sequencing was useful in resolving the complex bacterial genomic structures in the de novo assemblies.

show abstract

Identification of Enterobacteriaceae isolates with OXA-48 and coproduction of OXA-181 and NDM-1 in Norway

Cited by 34 publications

References 14 publications

Worldwide Dissemination of the NDM-Type Carbapenemases in Gram-Negative Bacteria

Worldwide Dissemination of the NDM-Type Carbapenemases in Gram-Negative Bacteria

First Report of OXA-181-Producing Escherichia coli in China and Characterization of the Isolate Using Whole-Genome Sequencing

Whole-Genome Assembly of Klebsiella pneumoniae Coproducing NDM-1 and OXA-232 Carbapenemases Using Single-Molecule, Real-Time Sequencing

Contact Info

Product

Resources

About