Identification of enzymes from genus Trichoderma that can accelerate formation of ferulic acid and ethyl ferulate in collaboration with rice koji enzyme in sake mash

Iwamoto, Toshihiko; Sato, Anna; Takahashi, Itsuki; Ito, Takahito; Takano, Kouto; Noge, Koji; Okuda, Masaki; Hashizume, Katsumi

doi:10.1016/j.jbiosc.2019.01.014

Cited by 9 publications

(4 citation statements)

References 39 publications

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“…Evidence indicates that the regulatory effect of several substances on body functions depends on the treatment period [ 53 , 54 ]. For example, a longer period of treatment with Pimpinella anisum L. [ 53 ] and with ferulic acid [ 54 ], one of the ingredients contained in Rice- koji [ 55 ], was required to decrease systolic blood pressure compared to a high dose of them. The precise basis for the preventive roles of Rice- koji on enhanced pain-like behaviors in FST mice is unclear; however, improvements in neural functions in the brain might be due to the actions of EGT contained in Rice- koji , in part [ 56 ], because Rice- koji and EGT prevented enhanced formalin-evoked pain-like behaviors in the late, but not the early, phase in FST mice.…”

Section: Discussionmentioning

confidence: 99%

Preventive Roles of Rice-koji Extracts and Ergothioneine on Anxiety- and Pain-like Responses under Psychophysical Stress Conditions in Male Mice

Piriyaprasath,

Kakihara,

Kurahashi

et al. 2023

Nutrients

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This study determined the effect of daily administration of Rice-koji on anxiety and nociception in mice subjected to repeated forced swim stress (FST). In a parallel experiment, it was determined whether ergothioneine (EGT) contained in Rice-koji displayed similar effects. Anxiety and nociception were assessed behaviorally using multiple procedures. c-Fos and FosB immunoreactivities were quantified to assess the effect of both treatments on neural responses in the paraventricular nucleus of the hypothalamus (PVN), nucleus raphe magnus (NRM), and lumbar spinal dorsal horn (DH). FST increased anxiety- and pain-like behaviors in the hindpaw. Rice-koji or EGT significantly prevented these behaviors after FST. In the absence of formalin, both treatments prevented decreased FosB expressions in the PVN after FST, while no effect was seen in the NRM and DH. In the presence of formalin, both treatments prevented changes in c-Fos and FosB expressions in all areas in FST mice. Further, in vitro experiments using SH-SY5Y cells were conducted. Rice-koji and EGT did not affect cell viability but changed the level of brain-derived neurotrophic factor. In conclusion, Rice-koji could reduce anxiety and pain associated with psychophysical stress, possibly mediated by the modulatory effects of EGT on neural functions in the brain.

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Section: Discussionmentioning

confidence: 99%

Preventive Roles of Rice-koji Extracts and Ergothioneine on Anxiety- and Pain-like Responses under Psychophysical Stress Conditions in Male Mice

Piriyaprasath,

Kakihara,

Kurahashi

et al. 2023

Nutrients

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“…e glycoside hydrolase 10 (GH10) and glycoside hydrolase 11 (GH11) families are the two largest xylanase families [11]. Recent studies have pointed out that GH10 family enzymes can be applied in many fields, such as food, herb extraction, and animal feed [12][13][14]. Bacillus sp.…”

Section: Introductionmentioning

confidence: 99%

Improving the Extraction of Active Ingredients from Medicinal Plants by XynA Modification

Zhang

Xie

Teng

et al. 2022

Journal of Chemistry

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Active ingredients of medicinal plants have unique pharmacological and clinical effects. However, conventional extraction technology has many disadvantages, such as long-time and low-efficiency. XynA-assisted extraction may overcome such problems, since the plant cell wall is mainly composed of cellulose. Based on the three-dimensional protein structure, we found the C-terminal domain and N-terminal domain twisted together and resulted in more flexibility. We carried out a series of truncations, with XynA_ΔN36 getting more yields of active ingredients. As shown by HPLC analysis, the efficiencies for extraction of salvianic acid A and berberine from Salvia miltiorrhiza and Phellodendron chinense were increased by approximately 38.14% and 35.20%, respectively, compared with the conventional extraction protocol. The yields of the two compounds reached 2.84 ± 0.05 mg g−1 and 3.52 ± 0.14 mg g−1, respectively. Above all, XynA_ΔN36 can be applied to the extraction of salvianic acid A and berberine, and this study provides a novel enzyme for the extraction technology, which aids rational utilization and quality control of medicinal plants.

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“…During production, the plant cell walls must be depolymerized, which requires synergistic action of cellulase and xylanase to degrade the ingredients of plant cell walls including cellulose, hemicellulose, pectin, and lignin (4)(5)(6)(7). In addition to biofuel production, xylanase is also applied in various industries, such as the food industry, feed industry, paper industry, and brewing industry (8)(9)(10)(11).…”

Section: Introductionmentioning

confidence: 99%

Structural and biochemical characterization of bifunctional XynA

Xie

Liu

et al. 2020

Preprint

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Recently, a novel GH10 family enzyme XynA identified from Bacillus sp. is found to degrade both cellulose and xylan. To understand its molecular catalytic mechanism, here we first solve the crystal structure of XynA at 2.3 Å. XynA is characterized with a classic (α/β)8 TIM-barrel fold (GH10 domain) flanked by the flexible N-terminal domain and C-terminal domain. Circular dichroism, protein thermal shift and enzyme activity assays reveal that conserved residues Glu182 and Glu280 are both important for catalytic activities of XynA, which is verified by the crystal structure of XynA with E182A/E280A double mutant. Molecular docking studies of XynA with xylohexaose and cellohexaose as well as site-directed mutagenesis and enzyme activity assay demonstrat that Gln250 and His252 are indispensible to cellulase and bifunctional activity, separately. These results elucidate the structural and biochemical features of XynA, providing clues for further modification of XynA for industrial application.

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Identification of enzymes from genus Trichoderma that can accelerate formation of ferulic acid and ethyl ferulate in collaboration with rice koji enzyme in sake mash

Cited by 9 publications

References 39 publications

Preventive Roles of Rice-koji Extracts and Ergothioneine on Anxiety- and Pain-like Responses under Psychophysical Stress Conditions in Male Mice

Preventive Roles of Rice-koji Extracts and Ergothioneine on Anxiety- and Pain-like Responses under Psychophysical Stress Conditions in Male Mice

Improving the Extraction of Active Ingredients from Medicinal Plants by XynA Modification

Structural and biochemical characterization of bifunctional XynA

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