Identification of epidermal growth factor-secreting cells in the anterior pituitary of lactating female rats

Mouihate, A; Verrier, Danièle; Lestage, Jacques

doi:10.1677/joe.0.1480319

Cited by 17 publications

(12 citation statements)

References 5 publications

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“…In a study by Ren et al [29], relatively few secretory cells were shown to exhibit EGF immunoreactivity in human anterior pituitaries, but their cell type was not determined in that study. The discrepancy in results between the present and previous studies is probably due to differences in animal species used, or physiological states examined, since EGF mRNA synthesis in rat pituitary glands is known to be altered under certain conditions, such as lactation or cold stress [27, 28], or upon treatment with estrogen [16, 27, 30]. These results indicate that somatotropes usually express EGF mRNA in rat and mouse pituitaries, and that EGF may play roles in regulating pituitary functions through the pituitary EGF-EGF receptor system.…”

Section: Discussionmentioning

confidence: 66%

“…Using in situ hybridization and immunocytochemistry, Fan et al [27]revealed that somatotropes and gonadotropes express EGF mRNA in rat pituitaries, and that cold stress induces EGF mRNA expression in corticotropes and thyrotropes. Moreover, a reverse hemolytic plaque assay subsequently demonstrated that EGF is secreted from all types of pituitary secretory cells in lactating rats [28]. In a study by Ren et al [29], relatively few secretory cells were shown to exhibit EGF immunoreactivity in human anterior pituitaries, but their cell type was not determined in that study.…”

Section: Discussionmentioning

confidence: 99%

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Identification of Epidermal Growth Factor mRNA-Expressing Cells in the Mouse Anterior Pituitary

et al. 2000

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Epidermal growth factor (EGF) produced within the pituitary gland is associated with the growth of pituitary cells in rats. The aim of the present study was to localize EGF- and EGF receptor-expressing cells, and to clarify the involvement of EGF in DNA replication in 2-month-old male mouse pituitary cells. In situ hybridization of the pituitaries of these mice demonstrated that EGF mRNA was expressed in the anterior and intermediate lobes. Within the anterior pituitary, EGF mRNA-expressing cells were medium-sized and round, and made up 40% of the total number of secretory cells. EGF receptor mRNA was only detected in anterior pituitary cells. Forty-seven percent anterior pituitary cells expressed EGF receptor mRNA. An immunocytochemical study showed that most somatotropes and some mammotropes expressed EGF mRNA. When anterior pituitary cells were enzymatically dissociated and cultured in serum-free medium, RT-PCR demonstrated both EGF mRNA and EGF receptor mRNA expression. Treatment with EGF (1 and 10 ng/ml) for 5 days stimulated DNA replication in mammotropes and corticotropes. These results indicate that the DNA replication in mammotropes and corticotropes is regulated by the paracrine and/or autocrine activity of EGF produced at least in part by these cell types themselves.

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Section: Discussionmentioning

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Identification of Epidermal Growth Factor mRNA-Expressing Cells in the Mouse Anterior Pituitary

et al. 2000

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“…2000). These cells functionally secrete EGF, confirmed on reverse hemolytic plaque assays and IHC of rat anterior pituitary plurihormonal cells (Mouihate & Lestage 1995 a , b , Mouihate et al . 1996 b ).…”

Section: Anterior Pituitary Tissuementioning

confidence: 64%

Expression and function of ErbB receptors and ligands in the pituitary

Cooper¹,

Vlotides²,

Fukuoka³

et al. 2011

Endocrine-Related Cancer

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The role of ErbB family in discreet pituitary functions is reviewed. Several ErbB receptor ligands, EGF, TGFα, and heregulin are differentially expressed in normal gonadotroph and lactosomatotroph lineages, and other elements of the anterior pituitary. ErbB receptors, i.e. EGFR and ErbB2, are also localized to the anterior pituitary with preferential EGFR lactosomatotroph expression. EGF regulates CRH and ACTH secretion and corticotroph proliferation as well as exhibiting autocrine and paracrine effects on gonadotrophs and on lactosomatotroph proliferation, gene and protein expression, and hormonal secretion. EGF and EGFR are expressed in both functioning and non-functioning pituitary adenomas, with higher expression in more aggressive tumor subtypes. ErbB2 receptor is detected in all tumor subtypes, particularly in invasive tumors. ErbB tyrosine kinase inhibitors regulate hormonal secretion, cell morphology, and proliferation in lactosomatotroph tumors, reflecting the emerging application of targeted pituitary therapeutics.

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“…The epidermal growth factor (EGF) family of growth factors is able to modulate the proliferation, secretion and differentiation of most endocrine glands (for review see Fisher & Lakshman 1990). EGF is normally present in the pituitary, where it appears to be produced by subpopulations of all hormone-producing cell types, and especially by gonadotropes and thyrotropes (Kasselberg et al 1985, Mouihate et al 1996. The epidermal growth factorreceptor (EGF-R) is also normally expressed by anterior pituitary cells (Fan & Childs 1995), with EGF binding sites being localized mainly to lactotrope and somatotrope cells (Chabot et al 1986).…”

Section: Introductionmentioning

confidence: 99%

Epidermal growth factor binding sites in human pituitary macroadenomas

Jaffrain-Rea

Petrangeli²,

Lubrano

et al. 1998

Journal of Endocrinology

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The number of epidermal growth factor (EGF) binding sites was determined by competitive binding assays in a series of 46 pituitary macroadenomas. A single concentration of 125 I-EGF (1 nM) was used for all experiments. In four cases, a displacement curve was obtained by adding increasing concentrations of cold EGF, and Scatchard analysis showed the presence of two classes of EGF binding sites, with K d1 =0·62 0·23 nM and K d2 =53·8 8·2 nM for the high-and low-affinity binding sites respectively. The distribution of EGF binding sites was studied in 42 cases by a single-point assay, in the presence and in the absence of a 100-fold cold EGF excess. A non-parametric distribution of EGF binding sites was observed (median 10·2 fmol/mg membrane protein, range 0·0-332·0). EGF-receptor positivity, defined as EGF binding d10·0 fmol/mg protein, was observed in 23 samples (54·8%), especially in prolactinomas (76·5%, P<0·05 vs other tumors taken together) and in gonadotrope adenomas (62·5%). EGF binding was higher in invasive than in non-invasive adenomas (median: 12·8 vs 0·0 fmol/mg membrane protein, P=0·047), and especially in adenomas invading the sphenoid sinus (median 26·7 fmol/mg membrane protein, P=0·008 vs other adenomas). EGF binding also tended to increase with the grade of supra/extrasellar extension according to Wilson (P=0·15). Sex steroid receptors (SSRs) were simultaneously determined in both cytosolic and nuclear fractions of 31 pituitary adenomas. Estrogen and progesterone receptors were determined by an enzyme-linked immunoassay and androgen receptors by a competitive binding assay with [3 H]methyltrienolone. No correlation could be found between EGF binding and either the gender and gonadal status of the patients, or the expression of SSRs by the adenomas. We conclude that the EGF family of growth factors may play a role in the evolution of a significant subset of human pituitary adenomas, especially in their invasiveness, and that a high EGF binding capacity may represent an additional marker of aggressiveness for these tumors. Sex steroids do not appear to have a significant role in the regulation of EGF binding in vivo in these tumors.

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Identification of epidermal growth factor-secreting cells in the anterior pituitary of lactating female rats

Cited by 17 publications

References 5 publications

Identification of Epidermal Growth Factor mRNA-Expressing Cells in the Mouse Anterior Pituitary

Identification of Epidermal Growth Factor mRNA-Expressing Cells in the Mouse Anterior Pituitary

Expression and function of ErbB receptors and ligands in the pituitary

Epidermal growth factor binding sites in human pituitary macroadenomas

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