Identification of Epigallocatechin-3-Gallate (EGCG) from Green Tea Using Mass Spectrometry

Abstract

In an era where humanity is reinstating its lost hope and expectation on natural products, green tea occupies quite a position for what it has proven to be, in its endeavors for human welfare and health. Epigallocatechin-3-gallate (EGCG) is the key to the vast biological activities of green tea. Green tea is no longer in the backdrop; it has emerged as the most viral, trending bioactive molecule when it comes to health benefits for human beings. This review focuses on the use of various analytical techniques for the analysis of EGCG. That which has been achieved so far, in terms of in vitro, pure component analysis, as well as those spikes in biological fluids and those in vivo in animal and human samples, was surveyed and presented. The use of MS-based techniques for the analysis of EGCG is elaborately reviewed and the need for improvising the applications is explained. The review emphasizes that there is plenty of room to explore matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) applications in this subject area.

Efficacy of anti‐sebum moisturizing cream containing 2% l‐carnitine and 5% epigallocatechin gallate in seborrhea: A randomized clinical trial

“…Comparing to the results of the previous studies, EGCG cream and combine cream in our study had greater effect on sebum reduction. It may be caused by the effects of the following factors: (1) The green tea compound contains several polyphenol compounds with approximately 40%–60% EGCG 21 . In fact, EGCG is the most potent polyphenol derivative along with the anti‐androgen activity which cannot be found in some derivatives 7,21 .…”

“…It may be caused by the effects of the following factors: (1) The green tea compound contains several polyphenol compounds with approximately 40%–60% EGCG 21 . In fact, EGCG is the most potent polyphenol derivative along with the anti‐androgen activity which cannot be found in some derivatives 7,21 . From previous both in vitro and in vivo studies, it was found that the concentration of green tea or EGCG potentially tended to affect its anti‐sebum efficacy 6,20 .…”

“…21 In fact, EGCG is the most potent polyphenol derivative along with the anti-androgen activity which cannot be found in some derivatives. 7,21 From previous both in vitro and in vivo studies, it was found that the concentration of green tea or EGCG potentially tended to affect its anti-sebum efficacy. 6,20 (2) The formulation of the topical drug as a moisturizing cream which nourishes the skin hydration can promote better absorption of the anti-sebum agent, resulting in a better therapeutic effect.…”

Plasma Gel Matrix as a Promising Carrier of Epigallocatechin Gallate for Regenerative Medicine

“…In general, conventional methods for the quantitative determination of EGCG use high-(or ultra-high-) performance liquid chromatography alone or in combination with mass spectrometry [20][21][22][23]. However, although sample types may be limited, a simple and convenient method using a spectrophotometer is available without expensive analytical devices [17].…”

Determination of (+)-Catechin and Antioxidant Activity in Faloak (Sterculia quadrifida R. Br) Stem Bark Infusion

“…Catechins are the most abundant polyphenols in tea which account for 70-80% of the total polyphenols. There are four types of catechins that are most dominant in tea, namely (-)-epicatechin (EC), (-)-epigallocatechin (EGC), (-)-epicatechin-3-O-gallate (ECG), and (-)-epigallocatechin -3-O-gallate (EGCG), where of the four types of catechins, the concentration of EGCG dominates as much as 50-80% of the total catechins (Koch et al, 2018;Sivanesan et al, 2022). Therefore, further studies are needed to identify the total catechins in the stem bark of S. quadri da so that further comparisons can be made.…”