Identification of epitopes in Indian human papilloma virus 16 E6: A bioinformatics approach

Identification of human papillomavirus-16 E6 variation in cervical cancer and their impact on T and B cell epitopes

“…Lower the percentile score showed the higher binding affinity of the predicted epitope or vice-versa. On analysis, one new epitope 80-88 CYSLYGTTL for HLA-A*24:02 was identified with good binding affinity (percentile value= 0.8), which was absent in two previous studies from India and China (Singh et al, 2011;Yao et al, 2013). The predicted epitope 26-34 IILECVYCK for the MHC-I allele HLA-A*03:01 and HLA-A*11:01 was found to be most common and this epitope was also reported in another study (Yao et al, 2013) for HLA-A*11:01 but not for HLA-A*03:01.…”

“…The predicted epitope 26-34 IILECVYCK for the MHC-I allele HLA-A*03:01 and HLA-A*11:01 was found to be most common and this epitope was also reported in another study (Yao et al, 2013) for HLA-A*11:01 but not for HLA-A*03:01. The epitope IILECVYCK was also not repoted by the Singh et al (Singh et al, 2011).…”

“…These interactions are influenced by variations in the MHC alleles or in processed epitopes. There are few studies which have been done for the epitope prediction for HPV-16 E6 protein (Mohabatkar, 2007;Singh et al, 2011;Yao et al, 2013). However, these studies predicted the epitope on submitted sequences from the National Center for Biotechnology Information (NCBI) not on the experimentally isolated HPV E6 sequence.…”

In silico Design of Discontinuous Peptides Representative of B and T-cell Epitopes from HER2-ECD as Potential Novel Cancer Peptide Vaccines

“…Based on that study they were able to introduce potent antitumor peptides. Furthermore, Singh et al (2011) and Wiwanitkit et al (2007) performed computational analysis of human papilloma virus and Lig A of Leptospira interrogans sequences to find T-cell epitopes using the bioinformatics tool MHCpred. In addition, Nair et al (2011), reported the identification of epitopes of Alcohol dehydrogenase from Curvularia lunata.…”

“…Therefore, in several studies, it has been shown that synthetic peptide vaccines containing a targeted B-cell epitope and a T-cell epitope generated substantial immune responses (Axelsena et al, 2011). In other words, recognition of antigenic epitopes either small T-cell epitopes or large conformational epitopes by B-cells is the key event of immune responses (Doytchinova and Flower, 2002;Singh et al, 2011). Moreover, up to 90% of B-cell epitopes are constituted by discontinuous epitopes on the surface of proteins.…”