2001
DOI: 10.1111/j.1751-0813.2001.tb10674.x
|View full text |Cite
|
Sign up to set email alerts
|

Identification of equine herpesvirus 3 (equine coital exanthema virus), equine gammaherpesviruses 2 and 5, equine adenoviruses 1 and 2, equine arteritis virus and equine rhinitis A virus by polymerase chain reaction

Abstract: The development and validation of a comprehensive panel of PCR diagnostic tests, predominantly for viruses causing equine respiratory disease, that can be completed within 8 hours from receipt of clinical samples, provides a major advance in the rapid diagnosis or exclusion diagnosis of these endemic equine virus diseases in Australia.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1

Citation Types

0
64
0
1

Year Published

2006
2006
2022
2022

Publication Types

Select...
9
1

Relationship

0
10

Authors

Journals

citations
Cited by 81 publications
(65 citation statements)
references
References 27 publications
0
64
0
1
Order By: Relevance
“…10,17 No EHV were detected at 3 days after inoculation, whereas EHV-5 nucleic acids were detected at 7 days. There was no cytopathic effect (CPE) evident in the culture that was positive by EHV-5 PCR; thus, the virus was serially passaged repeatedly on RK-13 cells, with frequent monitoring for CPE.…”
mentioning
confidence: 97%
“…10,17 No EHV were detected at 3 days after inoculation, whereas EHV-5 nucleic acids were detected at 7 days. There was no cytopathic effect (CPE) evident in the culture that was positive by EHV-5 PCR; thus, the virus was serially passaged repeatedly on RK-13 cells, with frequent monitoring for CPE.…”
mentioning
confidence: 97%
“…The partial-length glycoprotein G (gG) gene, from nucleotides 802 to 1298, was amplified by PCR (Dynon et al, 2001) from DNA extracted from EHV-3 positive PVS from each mare, in both experiments, and unpurified PCR products were sequenced (Unidad de Genó mica, Instituto de Biotecnología, CICVyA, INTA-Castelar). The obtained nucleotide sequence data were assembled and aligned using the BioEdit Sequence Alignment Editor V7.0.5 program (Hall, 1999).…”
Section: Characterization Of the Excreted Virus By Dna Sequencingmentioning
confidence: 99%
“…A qualitative semi-nested polymerase chain reaction (PCR) analysis for Equid herpesvirus (EHV)-1, -2, -4, and -5 3,8,11,13 was performed on DNA of TTW and lung biopsy, and was positive for EHV-2 and EHV-5 on TTW and only for EHV-5 on lung biopsy. Viral isolation attempts from lung biopsy on rabbit kidney (RK)13 cells were negative after 5 passages.…”
mentioning
confidence: 99%