Identification of extracellular lipases/esterases produced by Thermus thermophilus HB27: Partial purification and preliminary biochemical characterisation

Fuciños, Pablo; Abadín, C.M.; Sanromán, Á.; Longo, María A.; Pastrana, Lorenzo; Rúa, M. Luisa

doi:10.1016/j.jbiotec.2005.01.019

Cited by 63 publications

(50 citation statements)

References 22 publications

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“…Apart from the above-mentioned bacterial genera, Aeromonas caviae (Velu et al 2012), Acinetobacter calcoaceticus (Pratuangdejkul and Dharrnsthiti 2000), Microbacterium luteolum (Joseph et al 2012), Lactobacillus plantarum (Regalla et al 2002), Thermus thermophilus (Fucinos et al 2005), and Serratia marcescens (Abdou 2003) are a few other bacteria with substantial lipolytic capability. Some of these isolates are especially useful, since they exhibit extremophilic properties of either heat (Fucinos et al 2005;Velu et al 2012) or cold tolerance (Pratuangdejkul and Dharrnsthiti 2000).…”

Section: Sources Of Microbial Lipasesmentioning

confidence: 99%

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An insight into microbial lipases and their environmental facet

Kanmani¹,

Aravind²,

Kumaresan³

2014

Int. J. Environ. Sci. Technol.

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Lipases are serine hydrolases that catalyze the hydrolysis and synthesis of esters formed from glycerol and long-chain fatty acids, by acting at the oil-water interface. Lipases from microbial sources have received heightened attention for an array of industrial applications, and these enzymes have been well exploited in the environmental sector as well. In this article, we present an overview of microbial lipase, including the microorganisms from which it could be produced; the application of recombinant DNA technology tools to produce lipase with enhanced properties, the effective use of waste materials as substrates for lipase production; the usage of statistical tools to efficiently optimize the production medium; lipase purification strategies; and the immobilization of the enzyme on a variety of support materials. The next section of the article provides a gist of its application in diversified spheres and focusses exclusively on the environmentally relevant ones. Lipasecatalyzed esterification, transesterification, and interesterification reactions, an emerging area of green chemistry; lipase-mediated in vitro biopolymer synthesis and degradation; and the application of lipase for remediating fat and oil constituents in wastewater are dealt with in-depth. When its full potential is harnessed, the enzyme could play a pivotal role in environmental management.

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Section: Sources Of Microbial Lipasesmentioning

confidence: 99%

“…Some of these isolates are especially useful, since they exhibit extremophilic properties of either heat (Fucinos et al 2005;Velu et al 2012) or cold tolerance (Pratuangdejkul and Dharrnsthiti 2000).…”

Section: Sources Of Microbial Lipasesmentioning

confidence: 99%

An insight into microbial lipases and their environmental facet

Kanmani¹,

Aravind²,

Kumaresan³

2014

Int. J. Environ. Sci. Technol.

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“…Lipases are an important group of biotechnologically relevant enzymes, and properties such as their ability to perform specific biotransformations including esterification and transesterification and their stability have made them increasingly popular in the food, pharmaceutical, detergent, cosmetic and oleochemical industries (Park et al 2005;Grbavcic et al 2007;Gupta et al 2007). For many industrially important biotransformations, biotechnological interest in these enzymes has increased owing to these distinctive features (Chowdary et al 2001;Hamsaveni et al 2001;Krishna et al 2001;Rao and Divakar 2001;Zhang et al 2001;Castro et al 2004;Carvalho et al 2005;Fucinos et al 2005).…”

Section: Introductionmentioning

confidence: 99%

“…Because of their large potential in industrial applications such as in cosmetics and pharmaceuticals, the production of microbial lipases has increased in the past few decades (Elibol and Ozer 2000;Kamini et al 2000;Fucinos et al 2005). Microbial lipases have good substrate specificity, stability and selectivity, owing to which they have gained special attention for industry (Dutra et al 2008;Griebeler et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Thermostable lipase from novelPseudomonassp. VITSDVM1 isolated from bovine milk

Devi

Mohanasrinivasan

Chetna

et al. 2015

Frontiers in Life Science

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The main aim of this study was to identify lipase-producing bacteria from bovine milk samples. The strain possessing the highest lipase activity was identified by physiological and molecular characterization. 16S rRNA phylogenetic analysis showed highest similarity with Pseudomonas sp. and the strain was named Pseudomonas sp. VITSDVM1. The lipase productivity was improved by varying the utilization of substrates, i.e. banana and potato peel extracts, in the production medium. The results of optimization studies showed that lipase activity was maximum with groundnut oil, compared with the other carbon sources used, and with peptone as a nitrogen source. The optimum pH and temperature were 8 and 40°C, respectively. The optimum substrate concentration was 20% for the incubation period of 72 h with a maximum activity of 4.66 U mL −1 . The purified lipase enzyme analyzed by high-performance liquid chromatography revealed a retention time of 3.6 min. Hence, lipase production using agricultural waste such as banana and potato peels could be used in industrial applications.

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“…더불어, 미생물 배양조건은 다량의 isoenzyme을 생산하기 위하여 다양한 방법들을 제시하고 있다 [11]. 미생물을 이용한 효소 생산 과정에서 지질분해효소의 생 산량과 균체량과는 서로 간에 상관관계가 있지만, 효소 생산 이 미생물의 생장률과 반드시 비례하지는 않는다 [6][7][8]. 따라 서, 세포 생장과 효소분비를 촉진하는 배양조건(배지 조성, pH, 온도, 산소 농도)에 대해 연구하는 것은 효율적인 효소생 산을 위해 반드시 필요하다.…”

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Culture Conditions for Improving Extracellular Lipolytic Enzyme Production by a Novel Thermophilic Geobacillus sp. AR1

Park¹,

Jeon²

2013

Journal of Life Science

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A microorganism (strain AR1) producing an extracellular lipolytic enzyme was isolated from hot springs located in Beppu, Japan. Phylogenetic analysis based on the 16S rDNA sequence and biochemical studies indicated that AR1 belongs to the genus Geobacillus. This study focused on novel strategies to increase extracellular lipolytic enzyme production by this novel Geobacillus sp. AR1.Cultures of the AR1 strain grew within a wide temperature range (from 35 to 75°C); the optimum temperature was 65°C. The pH for optimal growth was 6.5, whereas the optimum pH for lipolytic enzyme production was 8.5. The presence of oils in the culture medium led to improvements in lipolytic enzyme activity. Soybean oil was the most efficient inducer, and it yielded better results when added in the exponential phase. On the other hand, the addition of chemical surfactants led to lipolytic enzyme production. Their addition to the culture could affect the location of the enzyme activity. The addition of Tween 20 in the stationary phase significantly increased the proportion of the extracellular enzyme activity. According to the results, following the addition of soybean oil and Tween 20 in the exponential and stationary phases, the extracellular lipolytic activity was increased 2.4-fold compared with that of a control.

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Identification of extracellular lipases/esterases produced by Thermus thermophilus HB27: Partial purification and preliminary biochemical characterisation

Cited by 63 publications

References 22 publications

An insight into microbial lipases and their environmental facet

An insight into microbial lipases and their environmental facet

Thermostable lipase from novelPseudomonassp. VITSDVM1 isolated from bovine milk

Culture Conditions for Improving Extracellular Lipolytic Enzyme Production by a Novel Thermophilic Geobacillus sp. AR1

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