Identification of extracellular vesicles and characterization of miRNA expression profiles in human blastocoel fluid

Abstract

In this study, for the first time, we demonstrated the presence of microRNAs and extracellular vesicles in human blastocoel fluid. The bioinformatic and comparative analyses identified the biological function of blastocoel fluid microRNAs and suggested a potential role inside the human blastocyst. We found 89 microRNAs, expressed at different levels, able to regulate critical signaling pathways controlling embryo development, such as pluripotency, cell reprogramming, epigenetic modifications, intercellular communication, cell adhesion and cell fate. Blastocoel fluid microRNAs reflect the miRNome of embryonic cells and their presence, associated with the discovery of extracellular vesicles, inside blastocoel fluid, strongly suggests their important role in mediating cell communication among blastocyst cells. Their characterization is important to better understand the earliest stages of embryogenesis and the complex circuits regulating pluripotency. Moreover, blastocoel fluid microRNA profiles could be influenced by blastocyst quality, therefore, microRNAs might be used to assess embryo potential in IVF cycles.

Histone Post-Translational Modifications and CircRNAs in Mouse and Human Spermatozoa: Potential Epigenetic Marks to Assess Human Sperm Quality

“…In line with results demonstrating the sponge activity of circRNAs towards miRNAs [240], circNAPEPLDiso1 has been suggested to physically interact with five miRNAs whose targets are potentially involved in several molecular mechanisms regulating the first stages of embryo development, most of them are known to be expressed in human oocytes, blastocysts and blastocoel fluid [284][285][286].…”

MicroRNA-96 Promotes Vascular Repair in Oxygen-Induced Retinopathy—A Novel Uncovered Vasoprotective Function

“…Conversely, overexpression of the miR-183/96/182 cluster resulted in a decreased production of nitrite and ROS by macrophage (Muraleedharan et al, 2019) and a neuroprotective role in the brain, by positively regulating glutathione (GSH) levels, a major tissue antioxidant (Kinoshita et al, 2014). Collectively, these studies support the possibility that miR-96 can be decreased in hyperoxic/ oxidative stress conditions; this does not exclude other mechanisms such as extracellular vesicles (Liu and Lu, 2015;Battaglia et al, 2019).…”

Association of oral dysbiosis with oral cancer development (Review)

“…In particular, the two latter epigenetic events are now been recognized as key mechanisms of neoplastic transformation and a plethora of diseases (12,87,150). In this context, recent studies have identified different miRNAs, a class of non-coding RNA of 20-22 nucleotides, associated with the development and progression of different tumors (151)(152)(153)(154)(155)(156)(157).…”