Identification of FadAB Complexes Involved in Fatty Acid β-Oxidation in Streptomyces coelicolor and Construction of a Triacylglycerol Overproducing strain

Abstract

Oleaginous microorganisms represent possible platforms for the sustainable production of oleochemicals and biofuels due to their metabolic robustness and the possibility to be engineered. Streptomyces coelicolor is among the narrow group of prokaryotes capable of accumulating triacylglycerol (TAG) as carbon and energy reserve. Although the pathways for TAG biosynthesis in this organism have been widely addressed, the set of genes required for their breakdown have remained elusive so far. Here, we identified and characterized three gene clusters involved in the β-oxidation of fatty acids (FA). The role of each of the three different S. coelicolor FadAB proteins in FA catabolism was confirmed by complementation of an Escherichia coliΔfadBA mutant strain deficient in β-oxidation. In S. coelicolor, the expression profile of the three gene clusters showed variation related with the stage of growth and the presence of FA in media. Flux balance analyses using a corrected version of the current S. coelicolor metabolic model containing detailed TAG biosynthesis reactions suggested the relevance of the identified fadAB genes in the accumulation of TAG. Thus, through the construction and analysis of fadAB knockout mutant strains, we obtained an S. coelicolor mutant that showed a 4.3-fold increase in the TAG content compared to the wild type strain grown under the same culture conditions.

Differential protein expression during growth on linear versus branched alkanes in the obligate marine hydrocarbon‐degrading bacterium Alcanivorax borkumensis SK2T

“…In the case of Streptomyces coelicolor FadAB complexes, low efficiency or narrow substrate specificity range of the complexes accounted for lower growth rates exhibited by two of the three complexes during growth on oleic acid as the sole carbon source. (Menendez‐Bravo et al, ).…”

Antibiotic Production and Antibiotic Resistance: The Two Sides of AbrB1/B2, a Two-Component System of Streptomyces coelicolor

“…The fadA1/B1 ( SCO6026/27 ) system was up-regulated at 36 h in the mutant strain. This system is involved in the final steps of fatty acid β-oxidation ( Menéndez-Bravo et al, 2017 ).…”

The Plant Pathogenic Bacterium Streptomyces scabies Degrades the Aromatic Components of Potato Periderm via the β-Ketoadipate Pathway

“…Therefore, ferulic acid might act as a signal to produce enzymes such as those of the fatty acids oxidation complex. This protein complex which breaks down CoA activated derivatives of fatty acids into acetyl-CoA by the β-oxidation pathway (Menendez-Bravo et al, 2017) might be important for the degradation of the aliphatic constituents of suberin. Interestingly, the second most abundant protein induced by the addition of ferulic acid, SCAB_3954, is encoded by a gene of unknown function that is located in a gene cluster dedicated to Coenzyme A biosynthesis.…”