Identification of fast and slow growing rhizobia nodulating soybean (<i>Glycine max</i>[L.] Merr) by a multiplex PCR reaction

Pastorino, Graciela N.; Alcántara, Virginia Martínez; Balatti, Pedro Alberto

doi:10.1016/s0378-1097(03)00796-1

Cited by 6 publications

(13 citation statements)

References 26 publications

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“…Thus, presence of the RSα sequence is not correlated with growth rate of peanut-nodulating strains. In contrast, in a study of soybean-nodulating rhizobia, the slow-growing strain B. japonicum was found to contain RSα sequence, while fast-growing strains such as Sinorhizobium fredii and S. xingiangensis did not [7].…”

Section: Resultsmentioning

confidence: 74%

“…Useful markers for competition assays must be simple, accurate, and economical. The RSα sequence can be used to discriminate soybean-nodulating strains [7]. One purpose of this study was to determine if RSα fragment can be used as a tool to evaluate nodule occupancy of peanut in competition assays.…”

Section: Resultsmentioning

confidence: 99%

“…One purpose of this study was to determine if RSα fragment can be used as a tool to evaluate nodule occupancy of peanut in competition assays. Amplification of RSα fragment was conducted for peanut-nodulating strains using the primers described by Pastorino et al [7]. When the RSα fragment exists, the expected size of the amplification product was ~900 bp.…”

Section: Resultsmentioning

confidence: 99%

“…Amplification of RSα was performed with primers forward SAL1 (5′-AGCGGGCGCGGATAGTTCTGTTG-3′) and reverse SAR2 (5′-GGCTCGGCTCTGTCGTTGTATGC-3′). Polymerase chain reaction (PCR) was performed as described previously [7]. Products obtained were subjected to horizontal electrophoresis on 2% agarose gel stained with ethidium bromide.…”

Section: Amplification Of Rsα Fragmentmentioning

confidence: 99%

“…The RSα sequence can be used to discriminate soybean-nodulating strains, through amplification reaction of the segment with specific primers. For example, B. japonicum was found to contain the RSα sequence, whereas fast-growing strains such as Sinorhizobium fredii and S. xingiangensis did not [7]. Taxonomic characterization of indigenous peanut-nodulating rhizobial strains, by methods based on analysis of genes coding for 16S rRNA, has been proposed [8].…”

Section: Introduction *mentioning

confidence: 99%

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Molecular diversity of peanut‐nodulating rhizobia in soils of Argentina

Bogino

Banchio

Giordano

2010

J. Basic Microbiol.

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RSalpha sequencing is a valuable tool for identification of bacterial strains, and for evaluating the genetic structure of indigenous rhizobial populations. The purpose of this study was to evaluate, qualitatively, the presence or absence of RSalpha fragment in peanut-nodulating strains isolated from plants grown at four sites in central Argentina. RSalpha fragment was found in only three of 26 indigenous strains, and in one of three inoculant strains analyzed. In contrast to results from studies of other symbiotic nitrogen-fixing bacteria, such as soybean-nodulating strains, no correlation was found between generation time and presence of RSalpha sequence. Phylogenetic analysis of the 16S rRNA gene sequence grouped peanut-nodulating strains into two clusters, Bradyrhizobium japonicum vs. B. elkanii, and showed divergence among strains positive for RSalpha sequence. Our results confirm the genetic diversity previously reported for various peanut-nodulating rhizobial strains, and indicate that the RSalpha fragment is not applicable as a marker or tool for competition assays at the field or ecological level.

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Section: Resultsmentioning

confidence: 74%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Amplification Of Rsα Fragmentmentioning

confidence: 99%

Section: Introduction *mentioning

confidence: 99%

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Molecular diversity of peanut‐nodulating rhizobia in soils of Argentina

Bogino

Banchio

Giordano

2010

J. Basic Microbiol.

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Detection of Rhizobium leguminosarum bv. trifolii and Rhizobium leguminosarum bv. phaseoli isolated from root nodules of leguminous plants by multiplex-PCR

Nawaf Al-zaidy,

Mohammed Hamed,

Nawaf Ahmed

2023

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The genetic diversity of 4 R. leguminosarum bv. trifolii and R. leguminosarum bv. Phaseoli that was extracted from root nodes of Trifolium spp and Phaseolus vulgaris L cultivars circulated in Mosul soils were investigated. The bacteria strains under study show similar tolerance levels against some external factors. In the current study, using classical diagnosis methods, similarity and distance indices and DNA polymorphism were examined with the randomly amplified polymorphic DNA. The isolates were distributed through genetic diversification into two main classes, including two main branches, Rl2 and the other sub-group was divided into Rl3, and the neighboring group involved Rl1 and Rl4. It is noted that individual plants and abiotic aspects were less affected than the Genetic factor on bacterial diversity. On the other hand, in multiplex-PCR reaction, Our outcomes revealed specific amplified for R. leguminosarum bv. trifolii with amplification products 419 bp and R. leguminosarum bv. Phaseoli with amplification products 362 bp. Keywords: R. leguminosarum bv. phaseoli, R. leguminosarum bv. trifolii , nodules, multiplex-PCR, RAPD-PCR .

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Replicate Once Per Cell Cycle: Replication Control of Secondary Chromosomes

et al. 2018

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Faithful vertical transmission of genetic information, especially of essential core genes, is a prerequisite for bacterial survival. Hence, replication of all the replicons is tightly controlled to ensure that all daughter cells get the same genome copy as their mother cell. Essential core genes are very often carried by the main chromosome. However they can occasionally be found on secondary chromosomes, recently renamed chromids. Chromids have evolved from non-essential megaplasmids, and further acquired essential core genes and a genomic signature closed to that of the main chromosome. All chromids carry a plasmidic replication origin, belonging so far to either the iterons or repABC type. Based on these differences, two categories of chromids have been distinguished. In this review, we focus on the replication initiation controls of these two types of chromids. We show that the sophisticated mechanisms controlling their replication evolved from their plasmid counterparts to allow a timely controlled replication, occurring once per cell cycle.

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Identification of fast and slow growing rhizobia nodulating soybean (Glycine max[L.] Merr) by a multiplex PCR reaction

Cited by 6 publications

References 26 publications

Molecular diversity of peanut‐nodulating rhizobia in soils of Argentina

Molecular diversity of peanut‐nodulating rhizobia in soils of Argentina

Detection of Rhizobium leguminosarum bv. trifolii and Rhizobium leguminosarum bv. phaseoli isolated from root nodules of leguminous plants by multiplex-PCR

Replicate Once Per Cell Cycle: Replication Control of Secondary Chromosomes

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