Identification of Fast-Growing Rhizobia Nodulating Tropical Legumes from Puerto Rico as Rhizobium gallicum and Rhizobium tropici

Zurdo-Piñeiro, José Luis; Velázquez, Encarna; Lorite, María J.; Brelles-Mariño, Graciela; Schröder, Eduardo C.; Bedmar, Eulogio J.; Mateos, Pedro F.; Martínez‐Molina, Eustoquio

doi:10.1078/0723202041438437

Cited by 46 publications

(32 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[6,[16][17][18] and archaea [6]. Moreover, the LMW RNA profiles are suitable for the analysis of large microbial populations [8,[12][13][14], and allow the detection of new species and genera [9,10,15]. These results are in agreement with those obtained by ribosomal DNA sequences and DNA-DNA hybridization, and a wide study of prokaryotic and fungal species showed that LMW RNA are molecular signatures of microbial species [6].…”

supporting

confidence: 87%

A new approach for separating low‐molecular‐weight RNA molecules by staircase electrophoresis in non‐sequencing gels

et al. 2006

Self Cite

View full text Add to dashboard Cite

Low-molecular-weight (LMW) RNA profiles, which include ribosomal and transfer RNA molecules with similar small sizes, are molecular signatures of microorganisms with a great potential in microbial identification. The greatest resolution of these profiles was achieved by staircase electrophoresis in sequencing gels. Nevertheless, this technique is difficult to use because it takes 7 h, the gels have large sizes and it is necessary to heat the system and to recycle the buffer to maintain the denaturing conditions and avoid smile effects. Most available sequencing slabs have no internal temperature control or homogenizing devices, which by contrast are present in some newly designed non-sequencing slabs. Nevertheless, these slabs present two important problems for separating LMW RNA molecules, the size of gels is only 20 cm (instead of 40 cm) and the maximum voltage that can be reached is only 840 V (instead 2400 V). Staircase electrophoresis follows a model in which the external polarization is incrementally modified with a constant time step value. In the present work, we experimentally confirmed that by reducing the time step and increasing the total number of steps a suitable resolution is achieved. Under these conditions, despite the smaller size of the gels and the lower values of the electric field, the intensity reaches higher values than in sequencing gels and the LMW RNA profiles are correctly separated in 5 h. The resolution of these profiles obtained in non-sequencing gels is similar to that obtained in sequencing ones facilitating the analysis of large populations of microorganisms in any laboratory.

show abstract

supporting

confidence: 87%

A new approach for separating low‐molecular‐weight RNA molecules by staircase electrophoresis in non‐sequencing gels

et al. 2006

Self Cite

View full text Add to dashboard Cite

show abstract

“…In some species, the nodulation genes have been detected as occurred in the case D. neptuniae that carry nodD and nifH genes closely related to those of R. tropici CIAT899 T (Rivas et al, 2002). The high identity of these genes suggested that they were transferred to D. neptuniae from R. tropici, an American species nodulating Leucaena (Martínez-Romero et al, 1991) and Neptunia in America (Zurdo-Piñeiro et al, 2004). Also the nifH and nodD genes were detected in plasmids using nifH and nodD probes in Ochrobactrum lupini (Trujillo et al, 2005) and P. trifolii (Valverde et al 2005).…”

Section: The New Rhizobial Speciesmentioning

confidence: 94%

Bacterial Associations with Legumes

Peix

Ramírez-Bahena

Velázquez

et al. 2014

Critical Reviews in Plant Sciences

Self Cite

318

212

View full text Add to dashboard Cite

“…Although a few species are native to Asia (e.g., Mimosa himalayana) and Africa (particularly Madagascar), most of the 480 or so species of Mimosa are native to Central and South America (2), with the Cerrado region of Central Brazil being the major center of diversification for the genus (2, 32). It has long been known that Mimosa plants may be nodulated by a variety of rhizobia (1, 37), but few have been typified, and prior to 2000, all had been ascribed to known ␣-rhizobial genera (3,25,27,42,47). Since the initial report of Moulin et al (26) on Burkholderia strains STM815 and STM678, many other strains of "␤-rhizobia" have been isolated, but the majority of them have come from Mimosa spp.…”

Section: Vol 71 2005mentioning

confidence: 99%

Proof that Burkholderia Strains Form Effective Symbioses with Legumes: a Study of Novel Mimosa -Nodulating Strains from South America

Chen

Faria

Straliotto

et al. 2005

Appl Environ Microbiol

165

175

View full text Add to dashboard Cite

Twenty Mimosa-nodulating bacterial strains from Brazil and Venezuela, together with eight reference Mimosa-nodulating rhizobial strains and two other ␤-rhizobial strains, were examined by amplified rRNA gene restriction analysis. They fell into 16 patterns and formed a single cluster together with the known ␤-rhizobia, Burkholderia caribensis, Burkholderia phymatum, and Burkholderia tuberum. The 16S rRNA gene sequences of 15 of the 20 strains were determined, and all were shown to belong to the genus Burkholderia; four distinct clusters could be discerned, with strains isolated from the same host species usually clustering very closely. Five of the strains (MAP3-5, Br3407, Br3454, Br3461, and Br3469) were selected for further studies of the symbiosisrelated genes nodA, the NodD-dependent regulatory consensus sequences (nod box), and nifH. The nodA and nifH sequences were very close to each other and to those of B. phymatum STM815, B. caribensis TJ182, and Cupriavidus taiwanensis LMG19424 but were relatively distant from those of B. tuberum STM678. In addition to nodulating their original hosts, all five strains could also nodulate other Mimosa spp., and all produced nodules on Mimosa pudica that had nitrogenase (acetylene reduction) activities and structures typical of effective N 2 -fixing symbioses. Finally, both wild-type and green fluorescent protein-expressing transconjugant strains of Br3461 and MAP3-5 produced N 2 -fixing nodules on their original hosts, Mimosa bimucronata (Br3461) and Mimosa pigra (MAP3-5), and hence this confirms strongly that Burkholderia strains can form effective symbioses with legumes.Although it was generally accepted for many years that legumes (and the nonleguminous plant Parasponia) were nodulated exclusively by members of the Rhizobiaceae in the ␣-Proteobacteria (including the genera Allorhizobium, Azorhizobium, Bradyrhizobium, Mesorhizobium, Rhizobium, and Sinorhizobium) (30, 34), recently there has been an increasing number of reports of members of the ␤-Proteobacteria being isolated from nodules. So far, these include Burkholderia tuberum strain STM678 and Burkholderia phymatum strain STM815 (originally isolated from Aspalathus carnosa in South Africa and Machaerium lunatum in French Guiana, respectively [26,39]), Ralstonia taiwanensis strains (isolated from Mimosa pudica in Taiwan and India and Mimosa diplotricha in Taiwan [8,41] and now renamed Cupriavidus taiwanensis [38]), and several Burkholderia strains isolated from Mimosa casta, Mimosa pigra (synonym, Mimosa pellita), M. pudica, and another mimosoid legume, Abarema macradenia, in Panama (3). Although symbiotic genes (nifH and nodA) have been identified in the Burkholderia strains STM678 and STM815, so far there is very little physiological and structural evidence of their symbiotic nature, and they have been shown to form only ineffective nodules on the promiscuous legume Macroptilium atropurpureum (26). More convincingly, not only have some of the Panamanian Burkholderia strains been shown to possess symbiosis-relat...

show abstract

Identification of Fast-Growing Rhizobia Nodulating Tropical Legumes from Puerto Rico as Rhizobium gallicum and Rhizobium tropici

Cited by 46 publications

References 0 publications

A new approach for separating low‐molecular‐weight RNA molecules by staircase electrophoresis in non‐sequencing gels

A new approach for separating low‐molecular‐weight RNA molecules by staircase electrophoresis in non‐sequencing gels

Bacterial Associations with Legumes

Proof that Burkholderia Strains Form Effective Symbioses with Legumes: a Study of Novel Mimosa -Nodulating Strains from South America

Contact Info

Product

Resources

About