Identification of Ferroptosis-Related Hub Genes and Their Association with Immune Infiltration in Chronic Obstructive Pulmonary Disease by Bioinformatics Analysis

Yang, Yi-Can; Zhang, Mengyu; Liu, Jian-Yu; Jiang, Yuanyuan; Ji, Xiu-Li; Qu, Yi-Qing

doi:10.2147/copd.s348569

Cited by 15 publications

(5 citation statements)

References 62 publications

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“…In a recent bioinformatics analysis, the ferroptosis-related gene HIF1A was one of the valid biomarkers for stomach adenocarcinoma [33]. In another study, HIF1A was identified as a ferroptosis-related hub gene that may affect the pathogenesis of chronic obstructive pulmonary disease via regulating ferroptosis [34]. SP1 is involved in most cell processes, such as cell differentiation, cell growth, apoptosis, immune responses, and response to DNA damage [35].…”

Section: Discussionmentioning

confidence: 99%

Investigating the Mechanism of Arsenic-induced Ferroptosis in the Skin

Koushki,

Amiri-Dashatan,

Rezaei

et al. 2024

IJMTFM

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Background: Ferroptosis, an oxidative and iron-dependent cell death, is a new type of regulated cell death. There are few studies on the mechanisms of ferroptosis in the skin and related diseases. Arsenic is shown to induce ferroptosis cell death. This study aimed to decipher the relationship between arsenic exposure and ferroptosis cell death in the skin. Methods: Arsenic-gene interactions were obtained. Then, skin-specific arsenic-gene interactions were screened. Ferroptosis-related genes were identified. Analysis of functional and biological interactions was performed to identify possible mechanisms. Results: The arsenic-gene interactions and the ferroptosis-related genes showed an overlap of 59 genes. Functional enrichment, protein-protein interaction, and transcription factor (TF)/miRNA target gene interaction analyses were used to look into the mechanism of arsenic-induced ferroptosis in the skin. ACTB, CTNNB1, HSPA8, SRC, RACK1, CD44, and SQSTM1 were identified as key proteins. Gene ontology analysis of these proteins indicated the mitochondrial morphology and functionality changes following arsenic-induced ferroptosis in the skin. HIF1A and SP1 TFs regulate a large number of genes compared to other TFs. Ten miRNAs with high interaction with ferroptosis-associated genes were identified. Conclusion: This work investigated the mechanism of arsenic-induced ferroptosis in the skin and identified key genes and regulators, and functional analysis highlighted the role of mitochondria in this skin exposure.

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Section: Discussionmentioning

confidence: 99%

Investigating the Mechanism of Arsenic-induced Ferroptosis in the Skin

Koushki,

Amiri-Dashatan,

Rezaei

et al. 2024

IJMTFM

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“…PTGS2, also known as cyclooxygenase 2, is closely associated with ferroptosis (Yang, Zhang, et al, 2022;Zhu et al, 2021). Therefore, we investigated whether ICAM1 participates in the regulation of PTGS2 expression and influences ferroptosis.…”

Section: Validation Of the Candidate Ppi Of Icam1-ptgs2mentioning

confidence: 99%

Integration of single‐cell RNA sequencing of endothelial cells and proteomics to unravel the role of ICAM1–PTGS2 communication in apical periodontitis: A laboratory investigation

Lv,

Luo,

et al. 2024

Int Endodontic J

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AimEndothelial cells (EDs) play a key role in angiogenesis and are associated with granulomatous lesions in patients with chronic apical periodontitis (CAP). This study aimed to investigate the diversity of EDs using single‐cell ribonucleic acid sequencing (scRNA‐seq) and to evaluate the regulation of intercellular adhesion molecule 1 (ICAM1) on the ferroptosis‐related protein, prostaglandin‐endoperoxide synthase 2 (PTGS2), in CAP.MethodologyEDs from the uploaded scRNA‐seq data of five CAP samples (GSE181688 and GSE197680) were categorized using distinct marker genes. The interactions between vein EDs (veinEndo) and other cell types were analysed using CellPhoneDB. Differentially expressed proteins in the proteomics of human umbilical vein EDs (HUVECs) and THP‐1‐derived macrophages infected with Porphyromonas gingivalis were compared with the differentially expressed genes (DEGs) of VeinEndo in scRNA‐seq of CAP versus healthy control periodontal tissues. The protein–protein interaction of ICAM1–PTGS2 in macrophages and HUVECs was validated by adding recombinant ICAM1, ICAM1 inhibitor and PTGS2 inhibitor using real‐time polymerase chain reaction (PCR), western blotting, and immunofluorescence staining.ResultsEDs in patients with CAP were divided into eight subclusters: five vein ED, capillaries, arterials and EC (PLA). There were 29 mutually upregulated DEGs and two mutually downregulated DEGs in vein cells in the scRNA‐seq data, as well as differentially expressed proteins in the proteomics of HUVECs. Real‐time PCR and immunofluorescence staining showed that ICAM1 and PTGS2 were highly expressed in CAP, infected HUVECs, and macrophages. Recombinant protein ICAM1 may improve PTGS2 expression, reactive oxygen species (ROS), and Fe2+ levels and decrease glutathione peroxidase 4 (GPX4) and SLC7A11 protein levels. ICAM1 inhibitor may inverse the above changes.ConclusionsscRNA‐seq revealed the diversity of EDs in CAP and identified the possible regulation of ICAM1 by the ferroptosis‐related protein, PTGS2, in infected HUVECs and macrophages, thus providing a basis for therapeutic approaches that target the inflammatory microenvironment of CAP.

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“…Inhibition of myostatin binding to the hypoxia-inducible factor 2alpha (HIF2α) receptor resulted in partial restoration of glutathione peroxidase 4 and glutathione expression as well as simultaneous inhibition of ferroptosis. HIF2α, a key gene associated with ferroptosis in COPD, has been implicated in the pathogenesis of COPD by regulating immune cell infiltration and promoting inflammatory responses [74]. However, the precise mechanisms through which myostatin mediates ferroptosis by the HIF2α pathway in skeletal muscles in COPD remain unclear.…”

Section: Myokines Regulate the Balance Between Skeletal Muscle Protei...mentioning

confidence: 99%

Revisiting Skeletal Muscle Dysfunction and Exercise in Chronic Obstructive Pulmonary Disease: Emerging Significance of Myokines

Han,

Li,

et al. 2023

Aging and disease

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Skeletal muscle dysfunction (SMD) is the most significant extrapulmonary complication and an independent prognostic indicator in patients with chronic obstructive pulmonary disease (COPD). Myokines, such as interleukin (IL)-6, IL-15, myostatin, irisin, and insulin-like growth factor (IGF)-1, play important roles in skeletal muscle mitochondrial function, protein synthesis and breakdown balance, and regeneration of skeletal muscles in COPD. As the main component of pulmonary rehabilitation, exercise can improve muscle strength, muscle endurance, and exercise capacity in patients with COPD, as well as improve the prognosis of SMD and COPD by regulating the expression levels of myokines. The mechanisms by which exercise regulates myokine levels are related to microRNAs. IGF-1 expression is upregulated by decreasing the expression of miR-1 or miR-29b. Myostatin downregulation and irisin upregulation are associated with increased miR-27a expression and decreased miR-696 expression, respectively. These findings suggest that myokines are potential targets for the prevention and treatment of SMD in COPD. A comprehensive analysis of the role and regulatory mechanisms of myokines can facilitate the development of new exercise-based therapeutic approaches for patients with COPD.

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Identification of Ferroptosis-Related Hub Genes and Their Association with Immune Infiltration in Chronic Obstructive Pulmonary Disease by Bioinformatics Analysis

Cited by 15 publications

References 62 publications

Investigating the Mechanism of Arsenic-induced Ferroptosis in the Skin

Investigating the Mechanism of Arsenic-induced Ferroptosis in the Skin

Integration of single‐cell RNA sequencing of endothelial cells and proteomics to unravel the role of ICAM1–PTGS2 communication in apical periodontitis: A laboratory investigation

Revisiting Skeletal Muscle Dysfunction and Exercise in Chronic Obstructive Pulmonary Disease: Emerging Significance of Myokines

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