Identification of Five Outer Membrane-Associated Proteins among Cross-Protective Factor Proteins of<i>Pasteurella multocida</i>

Tabatabai, Louisa B.; Zehr, Emilie S.

doi:10.1128/iai.72.2.1195-1198.2004

Cited by 27 publications

(23 citation statements)

References 13 publications

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“…However, the protective antigen was later identifi ed erroneously as the lipoprotein PlpB (Tabatabai and Zehr 2004 ), which shows similarity to methionine -binding proteins from many other bacterial species. In fact, the cross -protective antigen is PlpE, a lipoprotein with homologs in Mannheimia haemolytica and Actinobacillus pleuropneumoniae that stimulate protective immunity in cattle and pigs (Wu et al 2007 ).…”

Section: Individual Antigensmentioning

confidence: 99%

Pasteurella

Boyce

Harper

and

et al. 2010

Pathogenesis of Bacterial Infections in Animals

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Section: Individual Antigensmentioning

confidence: 99%

Pasteurella

Boyce

Harper

and

et al. 2010

Pathogenesis of Bacterial Infections in Animals

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“…Recently, Wu and colleagues reported that mice immunized with recombinant PlpB protein were not protected against challenge infection with 30 or 60 CFU of P. multocida X-73 serotype A:1 [14]. However, it was unclear whether or not the derived recombinant PlpB was the same protein as the 39 kDa protein that was cross-protective as described earlier by Rimler and Tobatabai [10,12]. Tobatabai and Zehr reported that the PlpB protein was a 30-kDa protein that appeared on the SDS gel at 39 kDa [12].…”

Section: Discussionmentioning

confidence: 93%

“…However, it was unclear whether or not the derived recombinant PlpB was the same protein as the 39 kDa protein that was cross-protective as described earlier by Rimler and Tobatabai [10,12]. Tobatabai and Zehr reported that the PlpB protein was a 30-kDa protein that appeared on the SDS gel at 39 kDa [12]. Nevertheless, Wu et al reported the PlpB protein with molecular mass of only 28 kDa and finally concluded that this PlpB was not the same protein as the reported 39-kDa cross protective protein [14].…”

Section: Discussionmentioning

confidence: 95%

“…Later, Tobatabai and Zehr identified this protein as the Pasteurella lipoprotein B, PlpB [12]. Recently, Wu and colleagues reported that mice immunized with recombinant PlpB protein were not protected against challenge infection with 30 or 60 CFU of P. multocida X-73 serotype A:1 [14].…”

Section: Discussionmentioning

confidence: 99%

“…The purified antigens of various chromatographic fractions obtained from P. multocida can induce active immune cross protection in turkeys, and sera from these turkeys passively cross protected naive poultry [10]. The identity of the 39-kDa protein was determined from the molecular masses of tryptic peptides of Pasteurella PlpB, as listed in the National Center for Biotechnology Information database for P. multocida [12].…”

mentioning

confidence: 99%

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Expression and Immunoprotective Property of a 39-kDa PlpB Protein of Pasteurella multocida

Chomnawang

Nabnuengsap

Kittiworakarn

et al. 2009

The Journal of Veterinary Medical Science

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ABSTRACT. Pasteurella multocida is a Gram-negative bacterium known to infect a wide range of domestic and wild animals. The increasing incidence of P. multocida isolated from cases of fowl cholera and hemorrhagic septicemia has led to a renewed interest in this pathogen as well as in the development of vaccines. In this study, PCR primers were designed to amplify the fragment of plpB gene from P. multocida FC-Pakchong (A:1). The purified PCR product of plpB gene consisting of 831 base pairs was inserted into the pGEX-5X-1 plasmid, which expressed the GST protein, and then transformed into E. coli. The purified fusion GST-PlpB protein showed a major band of about 63 kDa on SDS-PAGE gel. After enzyme cleavage, the recombinant PlpB protein appeared at the estimated size of 36 kDa. The recombinant GST-PlpB protein was tested for the toxicity in vivo. The results showed no toxicity in mice at the highest tested concentration of the protein. Moreover, the immunoprotective property of the recombinant GST-PlpB protein was determined in mice after subcutaneous immunization and challenge with an approximate dose of 50-100 LD 50 of P. multocida serotype A:1 and A:3,4. It was demonstrated that this subunit vaccine provided 20-30% survival rate after subcutaneous immunization and challenge with an approximate dose of 50-100 LD 50 of P. multocida serotype A:1 and A:3,4 whereas all of the non-immunized mice died from P. multocida infection. In conclusion, our data indicated that the PlpB protein may not be an appropriate target as a candidate subunit vaccine for P. multocida infection.KEY WORDS: 39-kDa outer membrane associated protein, fowl cholera, Pasteurella multocida, PlpB, subunit vaccine.

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Construction of genetically modified tobacco (Nicotiana tabacum cv. Petit Havana) harboring ompH(A:3) from Pasteurella multocida (A:3)

Jeong

Maeng

Song

et al. 2008

Biotechnol Bioproc E

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G Construction of Genetically Modified Tobacco (Nicotiana tabacum cv. Petit Havana) Harboring ompH(A:3) from Pasteurella multocida (A:3) = háóçåÖ=gÉçåÖI=hóìåÖJ~Ü=j~ÉåÖI=gáÜóìå=pçåÖI=pìåÖóçìä=eçåÖI=q~ÉóçìåÖ=`ÜìåÖI= = åÇ=jççëáâ=hïçåG= &GRCTVOGPV QH )GPGVKE 'PIKPGGTKPI 5WPIM[WPMYCP 7PKXGTUKV[ 5WYQP -QTGCĜ Äëíê~Åí= Fowl cholera, caused by m~ëíÉìêÉää~=ãìäíçÅáÇ~=(A:3), is a fearsome disease leading to a nonproductive influence upon poultry industry. It has been known that outer membrane protein H (OmpH) in the bacterium is a strong candidate to bring on the notorious ailment. Genetically modified (GM) tobacco (káÅçíá~å~=í~Ä~Åìã cv. Petit Havana) harboring çãéeE^WPF was constructed to develop a plant expression system for the protein, OmpH(A:3). Some 987 bp-long (ORF with the stop codon, TAA) of the çãéeE^WPF=excluding the nucleotide for signal peptide, was amplified by RT-PCR with the gene specific primers= and pGEM-T-çãéeE^WPF as template DNA. The PCR-amplified DNA was ligated into _~ãHI/p~ÅI-cut pBI121 to obtain a recombinant plasmid, pBI121-çãéeE^WPF. It was then transformed into ^ÖêçÄ~ÅíÉêáìã=íìãÉÑ~ÅáÉåë=(LBA 4404) by liquid nitrogen method to generate a recombinant clone of ^ÖêçÄ~ÅíÉêáìã=LBA4404/pBI121-çãéeE^WPF. The ^ÖêçÄ~ÅíÉêáìã=LBA4404/ pBI121-çãéeE^WPF was inoculated into leaf discs of tobacco (2 day old). The gene-transfected leaves were cultured on Murashige-Skoog basal medium containing kanamycin (50 mg/mL) to generate numerous calli, from which some GM tobacco plants were obtained. Transgenicity of the tobacco plant was confirmed by PCR screening along with the DNA sequencing. Also, its expression in the GM-tobacco was examined qualitatively as well as quantitatively by ELISA/Western blot. These results suggest that the genetically modified tobacco plant can be potentially used as a model system to develop plantbased vaccine against the fowl cholera. © KSBB

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Identification of Five Outer Membrane-Associated Proteins among Cross-Protective Factor Proteins ofPasteurella multocida

Cited by 27 publications

References 13 publications

Pasteurella

Pasteurella

Expression and Immunoprotective Property of a 39-kDa PlpB Protein of Pasteurella multocida

Construction of genetically modified tobacco (Nicotiana tabacum cv. Petit Havana) harboring ompH(A:3) from Pasteurella multocida (A:3)

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