Identification of fly eggs using scanning electron microscopy for forensic investigations

Mendonça, Paloma Martins; Santos-Mallet, Jacenir Reis dos; Mello, Rubens Pinto de; Gomes, Leonardo; Queiroz, Margareth Maria de Carvalho

doi:10.1016/j.micron.2008.01.014

Cited by 29 publications

(26 citation statements)

References 17 publications

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“…However, the immature stages pose a great identification challenge for forensic entomologists due to their lack of species-specific anatomical characters (McDonagh et al 2009). Identification of these stages using traditional methods (Mendonca et al 2008) and/ or advanced techniques (McDonagh et al 2009) is therefore the focus of many forensic studies. The most popular method of identification in recent years includes molecular taxonomy (Harvey et al 2003; Smith and Baker 2008).…”

Section: Introductionmentioning

confidence: 99%

Utility of Multi-Gene Loci for Forensic Species Diagnosis of Blowflies

Zaidi

Wei

Shi

2011

Journal of Insect Science

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Contemporary studies in forensic entomology exhaustively evaluate gene sequences because these constitute the fastest and most accurate method of species identification. For this purpose single gene segments, cytochrome oxidase subunit I (COI) in particular, are commonly used. However, the limitation of such sequences in identification, especially of closely related species and populations, demand a multi-gene approach. But this raises the question of which group of genes can best fulfill the identification task? In this context the utility of five gene segments was explored among blowfly species from two distinct geographic regions, China and Pakistan. COI, cytochrome b (CYTB), NADH dehydrogenase 5 (ND5), nuclear internal transcribed spacers (ITS1 and ITS2), were sequenced for eight blowfly species including Chrysomya megacephala F. (Diptera: Calliphoidae), Ch. pinguis Walker, Lucilia sericata Meigen L. porphyrina Walker, L. illustris Meigen Hemipyrellia ligurriens Wiedemann, Aldrichina grahami Aldrich, and the housefly, Musca domestica L. (Muscidae), from Hangzhou, China; while COI, CYTB, and ITS2 were sequenced for four species, i.e. Ch. megacephala, Ch. rufifacies, L. cuprina, and the flesh fly, Sarcophaga albiceps Meigen (Sarcophagidae), from Dera Ismail Khan Pakistan. The results demonstrate a universal utility of these gene segments in the molecular identification of flies of forensic importance.

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Section: Introductionmentioning

confidence: 99%

Utility of Multi-Gene Loci for Forensic Species Diagnosis of Blowflies

Zaidi

Wei

Shi

2011

Journal of Insect Science

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“…The chorion of the egg shell in many insects bears more or less hexagonal honey-comb impressions of the follicular cells (epithelium) of the female ovaries (Mendonca et al 2008). Similar observations on chorion sculpture as reflection of the follicular cell morphology were also made by Ubero-Pascal and Puig (2007).…”

Section: Discussionmentioning

confidence: 99%

Egg Morphology of Twelve Species of Melolonthinae and Rutelinae (Coleoptera: Scarabaeidae)

Sreedevi

Tyagi

Ramamurthy

2015

The Coleopterists Bulletin

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“…Also, some of the published descriptions should be used with caution, as divergent information has been provided by independent authors. Such discrepancies can be found in descriptions of the egg morphology of Lucilia cuprina (Wiedemann) provided by Sukontason et al (2007) and Mendonça et al (2008). According to Sukontason et al (2007) in L. cuprina the plastron covers almost the entire egg length, whereas Mendonça et al (2008) for the same species described the plastron only in the anterior third of the dorsal surface.…”

Section: Discussionmentioning

confidence: 99%

“…Such discrepancies can be found in descriptions of the egg morphology of Lucilia cuprina (Wiedemann) provided by Sukontason et al (2007) and Mendonça et al (2008). According to Sukontason et al (2007) in L. cuprina the plastron covers almost the entire egg length, whereas Mendonça et al (2008) for the same species described the plastron only in the anterior third of the dorsal surface. Greenberg and Singh (1995) stated that egg morphology of L. sericata (Meigen), L. illustris (Meigen), L. cuprina, and L. coeruleiviridis Macquart (all as Phaenicia Robineau‐Desvoidy) is very similar and can cause difficulties in species identification.…”

Section: Discussionmentioning

confidence: 99%

“…Because of considerable medical, veterinary, and forensic importance, the morphology of immature stages of some of the more anthropophilic species of Calliphoridae has been relatively well studied to date (Bonatto and Carvalho, 1996; Erzinçlioğlu, 1989; Greenberg and Singh, 1995; Kitching, 1976; Mendonça et al, 2008, 2010; Peterson and Newman, 1991; Richards and Morrison, 1972b; Sukontason et al, 2004a, 2004b; Sukontason et al, 2007, 2008; Szpila et al, 2008; Tawfik and El‐Husseini, 1971; Thyssen and Linhares, 2007). On the other hand, egg morphology of many blowfly genera still has not been studied, preventing comprehensive comparisons of egg morphology at the genus level.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Egg morphology of nine species of pollenia robineau‐desvoidy, 1830 (Diptera: Calliphoridae)

Grzywacz

Szpila

Pape

2012

Microscopy Res & Technique

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Egg morphology of nine species of the cluster fly genus Pollenia Robineau-Desvoidy was studied with scanning electron microscopy. Funnel-shaped micropylar area, chorion covered with centrally depressed hexagons, well developed hatching pleats on the dorsal surface encompassing the median area were found in eggs of all species: P. amentaria (Scopoli), P. angustigena Wainwright, P. atramentaria (Meigen), P. labialis Robineau-Desvoidy, P. mayeri Jacentkovsky, P. pediculata Macquart, P. rudis (Fabricius), P. similis (Jacentkovsky), and P. vagabunda (Meigen). Untypical for Calliphoridae, a dual morphological structure of plastron-bearing area is described here for the first time. On the basis of our observations, all species studied share the presence of a plastron respiratory function on the entire surface between the hatching lines. Differences between species were found in the shape of the longitudinal hatching pleats and the proportion between 'island pattern' and 'hexagonal pattern' of their chorionic surface, and in the intensity of perforation of hexagons of the median area. Comparisons of Pollenia egg morphology with that of other representatives of Calliphoridae revealed its unique structure, allowing easy differentiation from other representatives of the family.

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Identification of fly eggs using scanning electron microscopy for forensic investigations

Cited by 29 publications

References 17 publications

Utility of Multi-Gene Loci for Forensic Species Diagnosis of Blowflies

Utility of Multi-Gene Loci for Forensic Species Diagnosis of Blowflies

Egg Morphology of Twelve Species of Melolonthinae and Rutelinae (Coleoptera: Scarabaeidae)

Egg morphology of nine species of pollenia robineau‐desvoidy, 1830 (Diptera: Calliphoridae)

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