2002
DOI: 10.1128/jcm.40.2.556-574.2002
|View full text |Cite
|
Sign up to set email alerts
|

Identification of Four Distinct Genotypes of Candida dubliniensis and Detection of Microevolution In Vitro and In Vivo

Abstract: The present study investigates further the population structure of Candida dubliniensis and its ability to exhibit microevolution. Using 98 isolates (including 80 oral isolates) from 94 patients in 15 countries, we confirmed the existence of two distinct populations within the species C. dubliniensis, designated Cd25 group I and Cd25 group II, respectively, on the basis of DNA fingerprints generated with the C. dubliniensis-specific probe Cd25. The majority of Cd25 group I isolates (48 of 71, 67.6%) were from … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1

Citation Types

11
134
3
1

Year Published

2002
2002
2019
2019

Publication Types

Select...
5
2

Relationship

1
6

Authors

Journals

citations
Cited by 79 publications
(149 citation statements)
references
References 58 publications
11
134
3
1
Order By: Relevance
“…We have chosen to determine the molecular details of the karyotype of CD36, the type strain and the source of the genome sequence. In contrast to the evidence for variation in vivo (and rarely in vitro) among C. dubliniensis strains (Gee, Joly et al 2002), the karyotype of CD36 has remained constant as determined by pulse-field electrophoresis for more than 10 years in our laboratory. During this time, three separate isolates have been maintained, including freezing, defrosting, growing, and refreezing, without any sign of change.…”
Section: Discussionmentioning
confidence: 62%
See 2 more Smart Citations
“…We have chosen to determine the molecular details of the karyotype of CD36, the type strain and the source of the genome sequence. In contrast to the evidence for variation in vivo (and rarely in vitro) among C. dubliniensis strains (Gee, Joly et al 2002), the karyotype of CD36 has remained constant as determined by pulse-field electrophoresis for more than 10 years in our laboratory. During this time, three separate isolates have been maintained, including freezing, defrosting, growing, and refreezing, without any sign of change.…”
Section: Discussionmentioning
confidence: 62%
“…2, lane 3). Strain 514 belongs to genotype group 3 of C. dubliniensis, while CD36 (lane 6) has a group 1 genotype (Gee, Joly et al 2002).…”
Section: Electrophoretic Karyotypes Of Other Strainsmentioning
confidence: 99%
See 1 more Smart Citation
“…However, identification of C. dubliniensis is hampered by its close relationship with Candida albicans, a situation that has sometimes led to the misidentification of isolates of C. dubliniensis as C. albicans (19). At present, the most accurate differentiation between isolates of the two species is performed in reference laboratories with the use of molecule-based techniques such as PCR or DNA fingerprinting with repetitive sequence-containing DNA probes (5,17,19). However, these sophisticated techniques are not suitable and often not readily applicable for use in small clinical mycology laboratories, where simple and rapid methods are needed.…”
mentioning
confidence: 99%
“…Conventional morphological and physiologic methods, as well as molecular techniques, were employed to confirm the identity of all isolates (1,2,4,5,11,19). All yeasts studied were initially grown for 48 h at 30°C on Sabouraud glucose agar (Difco, Detroit, Mich.).…”
mentioning
confidence: 99%