Identification of Four Genes from the Granaticin Biosynthetic Gene Cluster of Streptomyces violaceoruber Tue22 Involved in the Biosynthesis of L-Rhodinose.

Tornus, Diethild; Floss, Heinz G.

doi:10.7164/antibiotics.54.91

Cited by 17 publications

(11 citation statements)

References 44 publications

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“…The genes responsible were found downstream of med-ORF16 as a translationally coupled pair: med-ORF15 and med-ORF14. 49-Keto reduction is required for granaticin B (L-rhodinose) biosynthesis: the assigned gene, gra-ORF22 (Tornus et al, 2001), encodes a protein 45 % similar to the med-ORF15 product. Other homologues include urdZ3 for urdamycin A (47 % similarity, Hoffmeister et al, 2000), lanZ3 for landomycin (50 % similarity, Westrich et al, 1999) and snoG for nogalamycin biosynthesis in Streptomyces nogalater (45 % similarity, GenBank accession no.…”

Section: Post-pks Tailoring Steps In Aglycone Formationmentioning

confidence: 99%

Cloning, sequencing and heterologous expression of the medermycin biosynthetic gene cluster of Streptomyces sp. AM-7161: towards comparative analysis of the benzoisochromanequinone gene clusters

et al. 2003

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Medermycin is a Streptomyces aromatic C-glycoside antibiotic classified in the benzoisochromanequinones (BIQs), which presents several interesting biosynthetic problems concerning polyketide synthase (PKS), post-PKS tailoring and deoxysugar pathways. The biosynthetic gene cluster for medermycin (the med cluster) was cloned from Streptomyces sp. AM-7161. Completeness of the clone was proved by the heterologous expression of a cosmid carrying the entire med cluster in Streptomyces coelicolor CH999 to produce medermycin. The DNA sequence of the cosmid (36 202 bp) revealed 34 complete ORFs, with an incomplete ORF at either end. Functional assignment of the deduced products was made for PKS and biosynthetically related enzymes, tailoring steps including strereochemical control, oxidation, angolosamine pathway, C-glycosylation, and regulation. The med cluster was estimated to be about 30 kb long, covering 29 ORFs. An unusual characteristic of the cluster is the disconnected organization of the minimal PKS genes: med-ORF23 encoding the acyl carrier protein is 20 kb apart from med-ORF1 and med-ORF2 for the two ketosynthase components. Secondly, the six genes (med-ORF14, 15, 16, 17, 18 and 20) for the biosynthesis of the deoxysugar, angolosamine, are all contiguous. Finally, the finding of a glycosyltransferase gene, med-ORF8, suggests a possible involvement of conventional C-glycosylation in medermycin biosynthesis. Comparison among the three complete BIQ gene clusters – med and those for actinorhodin (act) and granaticin (gra) – revealed some common genes whose deduced functions are unavailable from database searches (the ‘unknowns’). An example is med-ORF5, a homologue of actVI-ORF3 and gra-ORF18, which was highlighted by a recent proteomic analysis of S. coelicolor A3(2).

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Section: Post-pks Tailoring Steps In Aglycone Formationmentioning

confidence: 99%

Cloning, sequencing and heterologous expression of the medermycin biosynthetic gene cluster of Streptomyces sp. AM-7161: towards comparative analysis of the benzoisochromanequinone gene clusters

et al. 2003

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“…After that, only a few functional studies have been carried out on this cluster. [2][3][4][5] The functions of several gra genes remain totally unknown. Knowledge about how the production is regulated is still limited.…”

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confidence: 99%

Streptomyces vietnamensis GIMV4.0001: a granaticin-producing strain that can be readily genetically manipulated

2011

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Granaticin belongs to a class of aromatic polyketides, benzoisochromanequinone antibiotics, among which actinorhodin is the most well-known member. The biosynthetic gene cluster of actinorhodin have been intensively studied and serves as a model for studying the type II polyketide synthatase (PKS) pathways. Granaticin has a similar basic skeleton but unique fine structure compared to actinorhodin (Figure 1). The opposite stereochemistry of the pyran ring and the unusual sugar attachment presented in granaticin structure has drawn much attention among chemical and biochemical researchers. The granaticin biosynthetic gene cluster (gra) was identified originally from Streptomyces violaceoruber Tü22, 1 and the functions of most biosynthetic genes were assigned based on sequence homology. After that, only a few functional studies have been carried out on this cluster. [2][3][4][5] The functions of several gra genes remain totally unknown. Knowledge about how the production is regulated is still limited. The fact that S. violaceoruber Tü22 is recalcitrant to plasmid transformation 1 has imposed a significant barrier to its study.S. vietnamensis GIMV4.0001 (CCTCC M 205143, hereafter referred as GIMV4.0001), the type strain of a newly designated streptomycete species by our laboratory, 6 was found to be a novel granaticin producer, and the gra cluster was sequenced by a sequential cloning strategy (unpublished result, GenBank accession number: GU233672). In this study, we report the establishment of an efficient and stable conjugation system between Escherichia coli and GIMV4.0001 and applying a modified PCR-targeted disruption method to GIMV4.0001.All strains and plasmids used in this study are listed in Supplementary Table S1. To screen putative exconjugants derived from GIMV4.0001, we first carried out an antibiotic sensitivity test using an agar diffusion method (Supplementary Information, Materials and methods). All the plates containing GIMV4.0001 showed clear zones of inhibition around the holes with apramycin, kanamycin, thiostrepton, streptomycin or spectinomycin at 10 mg ml À1 , suggesting that there were adequate choices of selectable marker for genetic manipulation of GIMV4.0001.Two kinds of plasmids, auto-replicating pHZ1358 and integrative pSET152, were used to test the feasibility to introduce foreign DNA into GIMV4.0001. The basic procedures of conjugation between E. coli and GIMV4.0001 were as described by Kieser et al., 7 but some modifications were made (Supplementary Information, Materials and methods). The optimal medium for conjugation was screened. Exconjugants were confirmed by PCR amplification with an aac3(IV)-specific primer pair (for pSET152; Supplementary Information, Table S2) or by plasmid isolation and restriction mapping (for pHZ1358). For pSET152, putative exconjugants were obtained from YD, SFM, YMS and Gauze's synthetic agar, but the efficiency differed. The conjugation frequency for YD, SFM, YMS and Gauze's synthetic agar were 8Â10 À4 , 3Â10 À4 , 5Â10 À6 and 2Â10 À5 , respectively. F...

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“…granaticin A, C bioactive polyketide [293,294] Streptomyces violaceoruber granatomycin E bioactive polyketide [293] kendomycin bioactive polyketide [295] metenaticin A, B and C bioactive polyketide [293] Streptoverticillium cinnamoneum antibiotic HA 94 antimicrobial substance [296] Streptoverticillium mobaraense…”

Section: Streptomyces Violaceorubermentioning

confidence: 99%

Database on the taxonomical characterisation and potential toxigenic capacities of microorganisms used for the industrial production of food enzymes and feed additives, which do not have a recommendation for Qualified Presumption of Safety

Benito

Ibáñez

Moncho

et al. 2017

EFSA Supporting Publications

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The present work constitutes the external scientific report of the EFSA open call OC/EFSA/FEED/2015/01. The aim of the call was to provide EFSA with a database from a review on the taxonomical description and potential toxigenic capacities of microorganisms used for the industrial production of feed additives and food enzymes. The review includes microorganisms used as source of feed additives and food enzymes for which EFSA has received or can potentially receive applications for safety assessment, and which have not been recommended for Qualified Presumption of Safety status. The database also comprises the molecular taxonomical identifiers and biosynthetic pathways involved in the production of toxic compounds and the responsible genes. The main result of the project is shown as a database developed according to the EFSA data structure. The methodological aspects and the queries used in the systematic search, as well as the procedure applied for the screening of scientific documents retrieved are described in this report. Details are available in supplementary appendices.In total, 22970 scientific documents were screened in the literature search, from which 411 were initially selected for providing pertinent data for the scope of the project. From the review of the selected articles, 474 bioactive secondary metabolites were recorded and 59 compounds were further studied in order to obtain data on their toxicology and the conditions in which they are produced by the microorganisms used in industrial fermentations. The database generated in this project comprises details that characterise, when available, the production conditions, genes involved and toxicity of these 59 compounds. This provides information that can be used to establish safety measures when using potentially toxigenic microorganisms in industrial fermentations Disclaimer: The present document has been produced and adopted by the bodies identified above as author(s). This task has been carried out exclusively by the author(s) in the context of a contract between the European Food Safety Authority and the author(s), awarded following a tender procedure. The present document is published complying with the transparency principle to which the Authority is subject. It may not be considered as an output adopted by the Authority. The European Food Safety Authority reserves its rights, view and position as regards the issues addressed and the conclusions reached in the present document, without prejudice to the rights of the authors. Acknowledgements:We thank the following EFSA satff: Jaime Aguilera, Margarita Aguilera-Gómez, Jane Richardson, Mario Monguidi and Davide Gibin for their valuable help and collaboration throughout the project. We also would like to thank the members of AINIA: Sonia Porta, Lidia Tomás, Joaquin Espí and Juan Antonio Nieto for providing expertise in biotechnology and molecular biology and contributing with their efforts to achieve the goals of this project, and Violeta Gómez from the University of Navarra for her colla...

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Identification of Four Genes from the Granaticin Biosynthetic Gene Cluster of Streptomyces violaceoruber Tue22 Involved in the Biosynthesis of L-Rhodinose.

Cited by 17 publications

References 44 publications

Cloning, sequencing and heterologous expression of the medermycin biosynthetic gene cluster of Streptomyces sp. AM-7161: towards comparative analysis of the benzoisochromanequinone gene clusters

Cloning, sequencing and heterologous expression of the medermycin biosynthetic gene cluster of Streptomyces sp. AM-7161: towards comparative analysis of the benzoisochromanequinone gene clusters

Streptomyces vietnamensis GIMV4.0001: a granaticin-producing strain that can be readily genetically manipulated

Database on the taxonomical characterisation and potential toxigenic capacities of microorganisms used for the industrial production of food enzymes and feed additives, which do not have a recommendation for Qualified Presumption of Safety

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