Identification of four mud crab species (genusScylla) using ITS-1 and 16S rDNA markers

Abstract

The first internal transcribed spacer (ITS-1) of nuclear ribosomal DNA and mitochondrial DNA 16S rRNA were amplified by Polymerase chain reaction (PCR) using genomic DNA extracted from adult tissue of four species of Scylla spp. and the first zoeal stages of S. serrata, S. paramamosain and S. olivacea as template. Using the ITS-1 region, variation in product fragment length was found to be useful for distinguishing S. serrata and S. olivacea from two other species. The other two species (S. paramamosain and S. tranquebarica) could be identified using the restriction endonuclease Hha I. Using 16S rDNA, all four species were identified using PCR-restriction fragment length polymorphism (RFLP) by double digestion with DraI and HindIII. These genetic markers can be used for hybridization breeding studies and in field studies of larval and juvenile mud crabs of the genus Scylla.

Life-history, movement, and habitat use of Scylla serrata (Decapoda, Portunidae): current knowledge and future challenges

“…Keenan et al (1998) revised the genus Scylla, combining genetic and morphometric data. Allozyme electrophoresis and analysis of mitochondrial DNA sequences (COI I and 16 S RNA) revealed four distinct species of Scylla, which are now generally accepted: S. serrata (Forskål, 1775), S. tranquebarica (Fabricius, 1789), S. olivacea (Herbst, 1796), and S. paramamosain (Estampador, 1949) (Fuseya & Watanabe, 1996;Keenan et al, 1998;Klinbunga et al, 2000;Imai et al, 2004;Fuseya et al, 2007;Lin et al, 2007;Ogawa et al, 2011;Mandal et al, 2014).…”

“…It can be found in tropical and subtropical coastal regions of the IWP including the southern coast of South Africa (34°S) (Du Plessis, 1971), the east coast of Africa (Le Reste et al, 1976;Barnes et al, 2002;Davis et al, 2004;Fondo et al, 2010;Macia et al, 2014), the Red Sea (Keenan et al, 1998), Gulf of Aden (Simões et al, 2001), the Arabian/ Persian Gulf (Hogarth & Beech, 2001;RezaieAtagholipour et al, 2013), South-east and East Asia (Bight of Bengal, 19-21°N, Mohanty et al, 2006;Zafar et al, 2006;Indonesia, 5°N-10°S, Sugama and Hutapea, 1999;Nordhaus et al, 2009), the northern Philippine Sea, (34°N) (Imai et al, 2004), and Australia (Keenan et al, 1998). It also occurs around oceanic islands of the Indo-Pacific: Marianas, Fiji, Samoa Islands (Keenan et al, 1998), and Seychelles, Maldives, and Mauritius (Gopurenko et al, 1999;Anand et al, 2006;Fratini et al, 2010).…”

EFFECT OF FOOD TYPES AND HABITATS ON THE VALUE OF FEMALE MATURITY INDEX (FMI) AND GONADO SOMATIC INDEX (GSI) IN THE EGG PRODUCTION OF MANGROVE CRABS, Scylla serrata

“…They are living in the inter and subtidal mangrove habitats and are benthic carnivores (Hill, 1980). The genus Scylla has four species namely serrata, tranquebarica, oceanica and paramamosain (Keenan et al, 1998, Imai et al, 2004. Two groups of Scylla are found in Indonesia, one is reddish or brownish green, the other is greyish green.…”

Genetic diversity of the Asian shore crab,Hemigrapsus sanguineus, in Korea and Japan inferred from mitochondrial cytochromecoxidase subunit I gene

“…Population genetic analyses of several crab species were performed in the 1990s using allozyme data (McMillen-Jackson et al 1994;Creasey et al 1997). The sequence analysis of mitochondrial DNA (mtDNA) is also considered a useful tool for phylogenic and systematic studies of closely related crab species (Schubart et al 1998;Imai et al 2004), and mtDNA has recently been used for intraspecific population genetic analyses (Roman and Palumbi 2004;Pfeiler et al 2005;Cassone and Boulding 2006). Although DNA markers are expected to overcome the deficiencies of allozyme analyses of population structure by increasing the accuracy and resolution of its analysis in sea crab species (McMillenJackson et al 1994;Creasey et al 1997), there have been few reports of the use of mtDNA to measure the genetic variation in shore crabs (Cassone and Boulding 2006).…”