Identification of four novel KIR2DL2 alleles and two novel KIR2DL3 alleles in an East African population

Hardie, Rae-Anne; Czarnecki, Chris; Ball, T. Blake; Plummer, Francis A.; Luo, Ma

doi:10.1016/j.humimm.2010.09.013

Cited by 10 publications

(13 citation statements)

References 16 publications

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“…KIR3DL1/S1 exons 1–5, 7, and 9, KIR2DL2/3 exon 4 [45], and HLA-B exons 2 and 3 [46] were amplified using genomic DNA with gene-specific primers (Table 5). PCR amplifications were confirmed by 1% agarose gel electrophoresis with ethidium bromide.…”

Section: Methodsmentioning

confidence: 99%

Enrichment of Variations in KIR3DL1/S1 and KIR2DL2/L3 among H1N1/09 ICU Patients: An Exploratory Study

Czarnecki

El-Gabalawy

et al. 2011

PLoS ONE

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BackgroundInfection by the pandemic influenza A (H1N1/09) virus resulted in significant pathology among specific ethnic groups worldwide. Natural Killer (NK) cells are important in early innate immune responses to viral infections. Activation of NK cells, in part, depend on killer-cell immunoglobulin-like receptors (KIR) and HLA class I ligand interactions. To study factors involved in NK cell dysfunction in overactive immune responses to H1N1 infection, KIR3DL1/S1 and KIR2DL2/L3 allotypes and cognate HLA ligands of H1N1/09 intensive-care unit (ICU) patients were determined.Methodology and FindingsKIR3DL1/S1, KIR2DL2/L3, and HLA -B and -C of 51 H1N1/09 ICU patients and 105 H1N1-negative subjects (St. Theresa Point, Manitoba) were characterized. We detected an increase of 3DL1 ligand-negative pairs (3DL1/S1+ Bw6+ Bw4−), and a lack of 2DL1 HLA-C2 ligands, among ICU patients. They were also significantly enriched for 2DL2/L3 ligand-positive pairs (P<0.001, Pc<0.001; Odds Ratio:6.3158, CI95%:2.481–16.078). Relative to St. Theresa aboriginals (STh) and Venezuelan Amerindians (VA), allotypes enriched among aboriginal ICU patients (Ab) were: 2DL3 (Ab>VA, P = 0.024, Pc = 0.047; Odds Ratio:2.563, CI95%:1.109–5.923), 3DL1*00101 (Ab>VA, P<0.001, Pc<0.001), 3DL1*01502 (Ab>STh, P = 0.034, Pc = 0.268), and 3DL1*029 (Ab>STh, P = 0.039, Pc = 0.301). Aboriginal patients ligand-positive for 3DL1/S1 and 2DL1 had the lowest probabilities of death (Rd) (Rd = 28%), compared to patients that were 3DL1/S1 ligand-negative (Rd = 52%) or carried 3DL1*029 (Rd = 52%). Relative to Caucasoids (CA), two allotypes were enriched among non-aboriginal ICU patients (NAb): 3DL1*00401 (NAb>CA, P<0.001, Pc<0.001) and 3DL1*01502 (CA

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Section: Methodsmentioning

confidence: 99%

Enrichment of Variations in KIR3DL1/S1 and KIR2DL2/L3 among H1N1/09 ICU Patients: An Exploratory Study

Czarnecki

El-Gabalawy

et al. 2011

PLoS ONE

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“…Many KIR2DL2/2DL3 alleles have been identified in world populations . Among the 18 alleles detected in this study, nine were novel alleles.…”

Section: Discussionmentioning

confidence: 58%

Allelic polymorphism of KIR2DL2/2DL3 in a southern Chinese population

Zhen

et al. 2015

Tissue Antigens

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KIR2DL2 and KIR2DL3 segregate as alleles of the same killer cell immunoglobulin-like receptor (KIR) gene locus. They have been associated with viral infectious diseases and certain cancers and their allelic information may help to better comprehend mechanisms. The allelic polymorphism of KIR2DL2/2DL3 has been shown to influence their binding specificity and affinity to the HLA-C1 ligands. The present study aims to investigate the distribution of the allelic polymorphism of KIR2DL2/2DL3 in a southern Chinese population using sequence-specific primer polymerase chain reaction (PCR-SSP) and PCR-sequence-based typing (SBT) at the entire coding sequence. Of the 306 tested individuals, 1.96% were positive for KIR2DL2 only, 78.10% for KIR2DL3 only, and 19.93% for both KIR2DL2 and 2DL3. KIR2DL3 showed a high degree of diversity in the study population with 15 alleles detected including 8 novel ones. The predominant 2DL3 allele in the study population is 2DL3*00101 (92.81%) followed by 2DL3*00201 (24.18%), 2DL3*023 (4.25%), and 2DL3*00109 (1.31%). The remaining 11 2DL3 alleles all had a frequency below 1%. Three detected 2DL2 alleles were 2DL2*00301 (18.95%), 2DL2*00101 (3.59%), and the novel 2DL2*013 (0.33%). These results provide further insight into the KIR gene diversity in Southern Chinese and may help to better understand the role played by KIR genes in associated diseases.

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“…PCR amplification for the coding region of KIR2DL2 and KIR2DL3. The complete coding sequences of the KIR2DL2 and KIR2DL3 gene including exon 1 to exon 9 were amplified using multiplex sets of primers from Hardie et al [13]. Exon 1 of KIR2DL2/2DL3 was amplified using primers located in the surrounding introns and sequenced using a primer nested within the amplified PCR product as described in Table 1.…”

Section: Methodsmentioning

confidence: 99%

Investigation of Killer Cell Immunoglobulin‐Like Receptors KIR2DL2 and KIR2DL3 Diversity and Identification of Ten Novel KIR2DL3 Alleles in the Chinese Han Population

Tao

Ying

et al. 2015

Scand J Immunol

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KIR2DL2 and KIR2DL3 are important inhibitory receptors that recognize a subset of HLA-C allelic products carrying Ser77 and Asn80. In this study, we have determined KIR2DL2 and KIR2DL3 diversity in the Chinese Han population by a PCR sequence-based typing. Based on sequencing, the coding regions of 166 Chinese Han individuals, seven new polymorphic sites (238G>A, 405G>A, 476A>G, 550G>A, 608G>A, 789T>C, 947T>C) were found.

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Identification of four novel KIR2DL2 alleles and two novel KIR2DL3 alleles in an East African population

Cited by 10 publications

References 16 publications

Enrichment of Variations in KIR3DL1/S1 and KIR2DL2/L3 among H1N1/09 ICU Patients: An Exploratory Study

Enrichment of Variations in KIR3DL1/S1 and KIR2DL2/L3 among H1N1/09 ICU Patients: An Exploratory Study

Allelic polymorphism of KIR2DL2/2DL3 in a southern Chinese population

Investigation of Killer Cell Immunoglobulin‐Like Receptors KIR2DL2 and KIR2DL3 Diversity and Identification of Ten Novel KIR2DL3 Alleles in the Chinese Han Population

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