Identification of four VP4 serological types (P serotypes) of bovine rotavirus using viral reassortants

Abstract

A series of five reassortant viruses each containing the VP4 gene of a distinct bovine rotavirus and the VP7 gene of human rotavirus strain ST3 was prepared, and antisera to these were produced in rabbits. In neutralization tests, these antisera allowed the differentiation of the five original strains (from three different VP7 or G serotypes) into three or possibly four VP4 or P serotypes. All of a further seven bovine rotavirus strains adapted to cell culture were successfully typed by these antisera. There was a degree of cross-reaction between antiserum to the fourth bovine rotavirus P serotype and the predominant human rotavirus serotype. However, antisera raised in guinea-pigs to recombinant VP4 from this serotype showed the bovine serotype to be distinct. There was no significant serological relationship between these four bovine rotavirus P serotypes and previously described P serotypes from rotaviruses isolated from man and non-bovine animals. The predominant bovine rotavirus VP7 serotypes G6 and G10 tended to have distinct P serotypes also.

A new serotype of the outer capsid protein VP4 shared by an unusual human rotavirus strain Ro1845 and canine rotaviruses

“…The prediction that the VP4 proteins of strains Ro1845, K9 and CU-1 represent a new P serotype was substantiated by neutralization assays with antisera to reassortant viruses containing the VP4 gene from each of Ro1845, CU-1 and RRV. Reassortants and antisera to them have been used successfully to determine the role played by each of VP7 and VP4 proteins in complex antigenic relationships among rotavirus strains (Hoshino et al, 1985;Gorziglia et al, 1990b;Matsuda et al, 1990;Snodgrass et al, 1992).…”

Rotavirus Diarrhea Outbreaks in Arabian Thoroughbred Foals in a Stud Farm, Turkey

“…At this date, at least six different P types (P [12], P[3], P [11], P [1], P [18] and P [7]) of equine rotaviruses has been reported 4, 13,17,18,29 . The known binary combinations in group A equine rotaviruses are especially G14P [12] 14 and G3P [12] 14 , followed P [1] with combination G10 and G8, P [7] with combination G13 17,20 .…”

Independent segregation of the VP4 and the VP7 genes in bovine rotaviruses as confirmed by VP4 sequence analysis of G8 and G10 bovine rotavirus strains

“…The presence of at least five P types in human rotaviruses has been noted using serological analysis with antisera against baculovirus-expressed VP4 proteins or VP4 sequence analysis (Gorziglia et al, , 1990aQian & Green, 1991;Taniguchi et al, 1989). Among bovine rotaviruses, three or four distinct P types were detected by neutralization tests with viral reassortants (Matsuda et al, 1991 ;Snodgrass et al, 1992). In both simian and porcine rotaviruses, the presence of two P types has been suggested, based on VP4 sequence analysis (Gorziglia et al, 1990b;Mackow et al, 1988;Nishikawa et al, 1988).…”

“…Matsuda et aI. (1991) and Snodgrass et al (1992) identified the VP4 of strains B223 and KK3 as the third P type of bovine rotavirus by serological analyses using viral reassortants. This sequence analysis confirmed their findings; the VP4 amino acid sequences of strains A44, B223 and KK3 demonstrated only 50 to 60 % similarity to those of any other rotaviruses reported so far.…”

“…In bovine rotaviruses serological and genetic analyses, in this and previous studies (Hardy et al, 1991 ;Huang et at., 1992;Snodgrass et al, 1990Snodgrass et al, , 1992Taniguchi et al, 1991), demonstrated that there also exist various combinations of G serotypes and P types, showing the independent segregation of VP4 and VP7 genes as follows: (i) many strains, represented by reference strain NCDV (G6 and NCDV-like P), (ii) Japanese strain 0510, U.S.A. strain B641 and reference strain UK (G6 and UK-like P), (iii) Thai strain A5 and Scottish strain J2538 (G8 and NCDV-like P), (iv) Scottish strain CP1 and Thai strain 61A (G10 and UKlike P) and (v) Japanese strain KK-3, Scottish strains E4046 and K2399, Australian strain B-11, U.S.A. strain B223 and Thai strain A44 (G10 and B223-1ike P). In addition, U.S.A. strain WC3 (G6) and Scottish strains 678 (G8) and V1005 (G10) appear to have the fourth bovine P type .…”