Identification of free radicals in oxidized and glycoxidized phosphatidylethanolamines by spin trapping combined with tandem mass spectrometry

Simões, Cláudia; Domingues, Pedro; Domingues, M. Rosário M.

doi:10.1002/rcm.6186

Cited by 7 publications

(3 citation statements)

References 33 publications

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“…Some studies provide evidence that glycated PE promote lipid peroxidation, partly through the generation of ROS that can be related with inflammation and other diabetes complications . GlucPE are more prone to undergo oxidation when compared with non‐GlucPE, and a recent study from our group showed that, besides the oxidation in the fatty acyl chain, GlucPE also undergo oxidative modification in the glucose moiety, rendering GlucPE more susceptible to oxidation . This synergy on the deleterious effects of oxidation and glycation may be related with atherosclerosis and other complications of diabetic patients with associated hyperglycemia .…”

Section: Introductionmentioning

confidence: 95%

“…[29,30] GlucPE are more prone to undergo oxidation when compared with non-GlucPE, and a recent study from our group showed that, besides the oxidation in the fatty acyl chain, GlucPE also undergo oxidative modification in the glucose moiety, rendering GlucPE more susceptible to oxidation. [31] This synergy on the deleterious effects of oxidation and glycation may be related with atherosclerosis and other complications of diabetic patients with associated hyperglycemia. [29,32] Only two studies reported the oxidation of glycated PE induced by hydroxyl radicals, [31,33] suggesting an important role of oxidative cleavage of the glucose moiety.…”

Section: Introductionmentioning

confidence: 99%

“…This synergy on the deleterious effects of oxidation and glycation may be related with atherosclerosis and other complications of diabetic patients with associated hyperglycemia . Only two studies reported the oxidation of glycated PE induced by hydroxyl radicals, suggesting an important role of oxidative cleavage of the glucose moiety. However, investigation on photooxidation of glycated PE is inexistent.…”

Section: Introductionmentioning

confidence: 99%

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Photooxidation of glycated and non‐glycated phosphatidylethanolamines monitored by mass spectrometry

et al. 2013

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Phosphatidylethanolamines (PE) are one of the major components of cells membranes, namely in skin and in retina, that are continuously exposed to solar UV radiation being major targets of photooxidation damage. In addition, due to the presence of the free amine group, PE can also undergo glycation, in hyperglycemic conditions which may increase the susceptibility to oxidation. The aim of this study is to develop a model, based on mass spectrometry (MS) analysis, to identify photooxidative degradation of selected PE (POPE: PE 16:0/18:1, PLPE: PE 16:0/18:2, PAPE: PE 16:0/20:4) and glycated PEs due to UV irradiation. Photooxidation products were analysed by electrospray ionization MS (ESI-MS) and tandem MS (ESI-MS/MS) in positive and negative mode. Emphasis is placed in the influence of glycation in the generation of distinct photooxidation products. ESI-MS spectra of PE after UV photo-irradiation showed mainly hydroperoxy derivatives, due to oxidation of unsaturated fatty acyl chains. Glycated PE gave rise to several new photooxidation products formed due to oxidative cleavages of the glucose moiety, namely between C1 and C2, C2 and C3, and C5 and C6 of this sugar unit. These new products were identified by ESI-MS/MS in positive mode showing distinct neutral loss depending on the different structure of the polar head group. These new identified advanced glycated photooxidation products may have a deleterious role in the etiology of diabetic retinopathy and in diabetic retinal microvascular complications.

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Section: Introductionmentioning

confidence: 95%