Identification of Fucosylated SERPINA1 as a Novel Plasma Marker for Pancreatic Cancer Using Lectin Affinity Capture Coupled with iTRAQ-Based Quantitative Glycoproteomics

Wu, Chia‐Chun; Lu, Yuting; Yeh, Ta‐Sen; Chan, Yun-Hsin; Dash, Srinivas; Yu, Jau‐Song

doi:10.3390/ijms22116079

Cited by 18 publications

(11 citation statements)

References 56 publications

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“…AAT acts on FN1 through Snail in colon and gastric cancer, resulting in epithelial-mesenchymal transformation, in turn, promoting cancer progression and metastasis [18,20]. In view of the elevated levels of AAT in both chronic pancreatitis and PDAC samples, we speculate that AAT also induces progression of chronic pancreatitis to PDAC through this mechanism [15,21]. The collective findings indicate that AAT not only is an acute phase reactive protein but also plays an important role in the acute pancreatitis-chronic pancreatitis-pancreatic cancer axis.…”

Section: Discussionmentioning

confidence: 95%

“…However, CA19-9 is also increased in benign pancre-atic and biliary diseases, such as obstructive jaundice, pancreatitis, cholangitis, and cancer of the stomach, colon, ovary, uterus, liver, and other organs [45,46]. In addition, 8-10% Caucasians with Lewis A-B genotype do not express CA19-9, indicative of limitations as a biomarker of pancreatic cancer [21].…”

Section: Discussionmentioning

confidence: 99%

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Differential Plasma Proteins Identified via iTRAQ-Based Analysis Serve as Diagnostic Markers of Pancreatic Ductal Adenocarcinoma

et al. 2023

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Objective. We aimed to identify differentially expressed proteins in the plasma of patients with pancreatic cancer and control subjects, which could serve as potential tumor biomarkers. Methods. Differentially expressed proteins were determined via isostatic labeling and absolute quantification (iTRAQ). Potential protein biomarkers were identified via enzyme-linked immunosorbent assay (ELISA) in 40 patients and 40 control subjects, and those eventually selected were further validated in 40 pancreatic cancer and normal pancreatic tissues. Results. In total, 30 proteins displayed significant differences in expression among which 21 were downregulated and 9 were upregulated compared with the control group. ELISA revealed downregulation of peroxiredoxin-2 (PRDX2) and upregulation of alpha-1-antitrypsin (AAT), Ras-related protein Rab-2B (RAB2B), insulin-like growth factor-binding protein 2 (IGFBP2), Rho-related GTP-binding protein RhoC (RHOC), and prelamin-A/C (LMNA) proteins in 40 other samples of pancreatic cancer. Notably, only AAT, RAB2B, and IGFBP2 levels were consistent with expression patterns obtained with iTRAQ. Moreover, all three proteins displayed a marked increase in pancreatic cancer tissues. Data from ROC curve analysis indicated that the diagnostic ability of AAT, RAB2B, and IGFBP2 combined with carbohydrate antigen 19-9 (CA19-9) for pancreatic cancer was significantly greater than that of the single indexes (area under the curve (AUC): 90% vs. 75% (CA19-9), 76% (AAT), 71% (RAB2B), and 71% (IGFBP2), all P < 0.01 ). Conclusion. AAT, RAB2B, and IGFBP2 could serve as effective biomarkers to facilitate the early diagnosis of pancreatic cancer.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Differential Plasma Proteins Identified via iTRAQ-Based Analysis Serve as Diagnostic Markers of Pancreatic Ductal Adenocarcinoma

et al. 2023

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“…Also, it was shown the higher levels of α-1-antitrypsin in nonrelapsing Hodgkin lymphoma (HL) compare to relapsing HL [48]. As far as posttranslational modification (PTMs) and proteoforms, different glycosylated patterns of serpin A1 were observed in lung cancer and PDAC patients [49,50]. Serpin A1 has 3 known splice-isoforms and 8 potential isoforms that are computationally mapped.…”

Section: Alpha-1-antitrypsin (A1at_human)mentioning

confidence: 99%

Construction of 2DE-Patterns of Plasma Proteins: Aspect of Potential Tumor Markers

Naryzhny¹,

Ронжина²,

Zorina³

et al. 2022

Preprint

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Cancer is a complex systemic disease that changes the entire proteome. The analysis of this transformation makes it possible to determine tumor markers, that is, the most characteristic biomacromolecules produced by tumor cells. Here, the question of finding ideal tumor markers, which should be sensitive, specific, and reliable, is an acute issue. Unfortunately, none of the tumor markers, even those used in the clinic, has all these characteristics. Despite this, many tumor markers have demonstrated excellent clinical relevance for monitoring the effectiveness of different treatments for cancer patients. The use of markers also aids in the early detection of cancer recurrence and prognosis. Therefore, the situation in this area can be improved in two ways – by attempting to find an ideal single tumor marker or generating panels of different markers. In both cases, proteomics certainly plays a major role. Human plasma is one of the most popular samples as it is commonly collected in the clinic and provides noninvasive, rapid analysis for any type of disease including cancer. Many efforts have been applied in searching for “ideal” tumor markers digging very deep plasma proteome. There is a line of evidence that the most abundant, so-called “classical plasma proteins”, may be used to generate a tumor biomarker profile. To be comprehensive these profiles should have information not only about protein levels but proteoform distribution for each protein. Initially, the profile of these proteins in norm should be generated. Here, we present data about these profiles generated by two-dimensional electrophoresis with the following mass-spectrometry and immunodetection.

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“…SERPINA1 (Serpin family A member 1) encodes a well-known elastase inhibitor, alpha-1 antitrypsin (AAT). Previously, Wiseman and his colleagues reported that SERPINA1 can be diagnostic biomarker of thyroid cancer [ 112 ], and SERPINA1 has been recently reported to be involved in several other cancers including gastric, liver, pancreas, breast, lung, and ovarian cancers [ 113 , 114 , 115 , 116 , 117 , 118 ]. Moreover, Gao and his colleagues suggested that SERPINA1 is one of key genes in brain metastasis from lung adenocarcinoma [ 119 ].…”

Section: Hsf2 Affects Cancer Development and Progressionmentioning

confidence: 99%

Heat Shock Transcription Factor 2 Is Significantly Involved in Neurodegenerative Diseases, Inflammatory Bowel Disease, Cancer, Male Infertility, and Fetal Alcohol Spectrum Disorder: The Novel Mechanisms of Several Severe Diseases

Tokunaga

Otsuyama

Kakuta

et al. 2022

IJMS

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HSF (heat shock transcription factor or heat shock factor) was discovered as a transcription factor indispensable for heat shock response. Although four classical HSFs were discovered in mammals and two major HSFs, HSF1 and HSF2, were cloned in the same year of 1991, only HSF1 was intensively studied because HSF1 can give rise to heat shock response through the induction of various HSPs’ expression. On the other hand, HSF2 was not well studied for some time, which was probably due to an underestimate of HSF2 itself. Since the beginning of the 21st century, HSF2 research has progressed and many biologically significant functions of HSF2 have been revealed. For example, the roles of HSF2 in nervous system protection, inflammation, maintenance of mitosis and meiosis, and cancer cell survival and death have been gradually unveiled. However, we feel that the fact HSF2 has a relationship with various factors is not yet widely recognized; therefore, the biological significance of HSF2 has been underestimated. We strongly hope to widely communicate the significance of HSF2 to researchers and readers in broad research fields through this review. In addition, we also hope that many readers will have great interest in the molecular mechanism in which HSF2 acts as an active transcription factor and gene bookmarking mechanism of HSF2 during cell cycle progression, as is summarized in this review.

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Identification of Fucosylated SERPINA1 as a Novel Plasma Marker for Pancreatic Cancer Using Lectin Affinity Capture Coupled with iTRAQ-Based Quantitative Glycoproteomics

Cited by 18 publications

References 56 publications

Differential Plasma Proteins Identified via iTRAQ-Based Analysis Serve as Diagnostic Markers of Pancreatic Ductal Adenocarcinoma

Differential Plasma Proteins Identified via iTRAQ-Based Analysis Serve as Diagnostic Markers of Pancreatic Ductal Adenocarcinoma

Construction of 2DE-Patterns of Plasma Proteins: Aspect of Potential Tumor Markers

Heat Shock Transcription Factor 2 Is Significantly Involved in Neurodegenerative Diseases, Inflammatory Bowel Disease, Cancer, Male Infertility, and Fetal Alcohol Spectrum Disorder: The Novel Mechanisms of Several Severe Diseases

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